Fluorescence microscopy - PowerPoint PPT Presentation


Advanced Techniques in Materials Science: Transmission Electron Microscopy

Explore the advanced techniques used in materials science, focusing on Transmission Electron Microscopy (TEM). Learn about the challenges with optical microscopes, the principles of TEM imaging, and the application of scattering theory in electron microscopy. Discover how TEM offers higher resolutio

3 views • 19 slides


Advanced Microscopy Techniques and Applications in Cell Biology

Explore the diverse world of advanced microscopy techniques in cell biology, from confocal and super-resolution imaging to Fluorescence Resonance Energy Transfer (FRET) microscopy and Fluorescence Recovery After Photobleaching. Discover the latest methods, considerations, and applications for studyi

3 views • 21 slides



Meticulous Research Unveils Comprehensive Insights into the Microscopy Market

Microscopy Market by Product (Microscopes, Software), Type [Optical (Compound, Digital), Electron (SEM, TEM), Scanning Probe (AFM, STM)], Application (Research, Material), and End User (Electronics, Healthcare, F&B, Academic) - Forecast to 2028

7 views • 2 slides


Meticulous Research Releases Comprehensive Analysis of the Microscopy Market

Microscopy Market Size, Share, Forecast, & Trends Analysis by Product (Microscopes (Optical [Compound, Stereo, Digital] Electron [SEM, TEM] Scanning Probe [AFM, STM]), Software) Application (R&D, Material Analysis) End User

0 views • 2 slides


Understanding Fluorimetry: Principles and Applications

Fluorimetry is the measurement of fluorescence intensity at a specific wavelength using instruments like filter fluorimeters. It involves the excitation of molecules by radiation, causing electron promotion and emission of radiation. This process includes states like singlet and triplet, with relaxa

3 views • 45 slides


Understanding Fluorimetry: Principles, Applications, and Instrumentation

Fluorimetry is a technique that measures fluorescence intensity of molecules when excited by radiation. It involves the promotion of electrons from ground to excited states, leading to emission of radiation. This process includes singlet and triplet states, as well as relaxation mechanisms like Coll

0 views • 45 slides


Understanding Fluorescence Analysis in Pharmaceutical Sciences

Fluorescence analysis is a crucial technique in pharmaceutical analysis, involving the emission of radiation by molecules when excited at specific wavelengths. Factors influencing fluorescence, such as concentration, light intensity, adsorption, oxygen presence, pH, temperature, viscosity, and photo

2 views • 26 slides


Basic Concepts of Microscopy for Materials Study

Microscopy plays a crucial role in examining the structural elements and defects influencing material properties. This involves studying microscopic features like grain size and shape using optical, electron, and scanning probe microscopes. Microstructural examinations help understand property-struc

1 views • 12 slides


Understanding Fluorescence Microscopy: Principles and Applications

Fluorescence microscopy, pioneered by British scientist Sir George G. Stokes, reveals hidden details in specimens using fluorescent dyes that emit light of longer wavelengths. This innovative technique allows for visualization of cellular components that are otherwise colorless under conventional mi

0 views • 19 slides


Understanding Microscopy and Micrometry in Veterinary Microbiology

Microscopy and micrometry play a crucial role in observing small organisms like microorganisms, making them visible for study. This article delves into the history, definition, types of microscopes, variables used in microscopy, and the significance of these tools in veterinary microbiology.

1 views • 15 slides


Principles and Applications of Centrifugation in Microtomy and Microscopy

Centrifugation is a process that separates substances based on density using high-speed rotation. This article explores the basic principles of centrifugation, instrumentation, and applications in the fields of microtomy and microscopy. It covers the different types of centrifuges, such as low-speed

0 views • 28 slides


Exploring the Fascinating World of Photophysics and Luminescence

Dive into the intriguing realm of photophysics, where various emission and loss processes lead to luminescence phenomena such as fluorescence, phosphorescence, and chemiluminescence. Discover the beauty of bioluminescence in nature, from fireflies to bioluminescent red tide events. Explore the mecha

0 views • 16 slides


NC22Plex STR Detection Kit: Advanced 5-Color Fluorescence Detection System

Explore the cutting-edge NC22Plex STR Detection Kit from Jiangsu Superbio Biomedical, offering a 5-color fluorescence detection system suitable for multiple applications. Enhance your research capabilities with this innovative product designed for precision and efficiency.

0 views • 20 slides


Understanding Phase Contrast Microscopy: A Revolutionary Tool in Cell Observation

Phase contrast microscopy, invented by Frits Zernike, revolutionized cell observation by utilizing tiny phase changes in light rays to create contrast in living cells. This technique exploits differences in refractive indices to enhance visibility without staining cells, offering a new perspective i

0 views • 27 slides


Analysis and Applications of Electron Microscopy in Science Education

Explore the in-depth analysis of quantitative and qualitative techniques in electron microscopy as it relates to interactions with matter, instrumentation, specimen preparation, elemental analysis, and more. Delve into the electromagnetic spectrum, radiation effects on the human body, and spectrosco

0 views • 13 slides


Bacteria Identification Techniques: Staining and Microscopy Overview

Staining techniques in microbiology play a crucial role in visualizing bacteria under microscopes. This content covers the importance of staining, types of dyes, wet and dry mounts, hanging drop technique, and different types of staining methods employed in identifying bacteria. Explore the basics o

0 views • 39 slides


Exploring Fluorescence Microscopy and High-Speed Cameras in Modern Biology

Delve into the fascinating world of fluorescence microscopy and high-speed cameras in biology through topics such as detectors for microscopy, Nyquist criterion, visualizing hearing in vivo, and temporal resolution insights. Learn about techniques, equipment, and practical considerations in utilizin

1 views • 69 slides


Proper Section Cutting Techniques for Microscopy

Learn about proper section cutting techniques for light and electron microscopy, including the ideal thickness for sections. Discover the essential steps for cutting paraffin sections and common faults to avoid. Find out the key points necessary for easy and successful sectioning, such as equipment

0 views • 4 slides


Understanding The Light Microscope: A Comprehensive Guide

Delve into the world of microscopy with Dr. Mohammed Hussein's Lab Manual No. 1, covering the fundamentals of the light microscope, its components, lenses, magnification power, resolving power, and detailed instructions on how to focus your microscope effectively for optimal results. Explore the his

0 views • 19 slides


Introduction to Histology and Microscopy in Anatomy

Histology is the study of tissue structure and function in animals and plants, focusing on the microscopic structure of cells, tissues, and organs. It helps in understanding the relationship between structure and function, intertwining disciplines like cell biology, pathology, physiology, and bioche

0 views • 5 slides


Understanding Chlorophyll Fluorescence in Plant Physiology

Chlorophyll fluorescence is a powerful tool developed in the 1990s for studying plant physiology and stress. It provides insights into photosynthetic processes at different levels, from subcellular to canopy. By measuring chlorophyll fluorescence, researchers can assess the efficiency of photosynthe

0 views • 16 slides


Understanding the Compound Light Microscope and Its Applications

Explore the fundamental concepts of microscopy through Professor Diane Hilker's detailed lectures on the use and care of the compound light microscope. Dive into topics like magnification, resolution, and the practical application of microscopes in scientific research. Enhance your understanding of

0 views • 17 slides


Advanced Microscopy Techniques in EUV Lithography: SHARP Overview

SHARP utilizes Fresnel zone plate lenses to achieve diffraction-limited quality in EUV lithography, offering a range of NA values and image magnifications. The system allows emulation of mask-side imaging conditions with hundreds of lenses available. Coherence control and engineering are provided th

1 views • 18 slides


Optimised Live/Dead Fluorescence Spectrometry for Antimicrobial Susceptibility Testing

This study focuses on the optimisation of live/dead fluorescence spectrometry for rapid antimicrobial susceptibility testing. The research uses fluorescent dyes to distinguish between live and dead cells, providing a potential method for quick antimicrobial resistance diagnostics. The experiment inv

0 views • 16 slides


Laboratory Diagnosis of Malaria: Methods and Procedures

Methods for diagnosing malaria in laboratory settings involve techniques such as light microscopy and rapid diagnostic tests. Light microscopy is used to prepare blood films, enabling the detection of malaria parasites like Plasmodium falciparum. Rapid diagnostic tests can quickly identify malaria a

0 views • 26 slides


Applications and Importance of X-ray Fluorescence Spectroscopy in Analytical Chemistry

X-ray Fluorescence Spectroscopy (XRF) is a vital analytical technique used for qualitative and quantitative analysis of elements based on their X-ray emission characteristics. Dr. Uma Sharma, a Professor at Vikram University, details various X-ray analytical methods, including X-ray emission, Auger

0 views • 21 slides


Understanding Cell Structure and Microscopy

Explore the world of cell biology and microscopy through a quiz covering topics such as cell structure, iodine use in the laboratory, magnification in microscopes, and more. Test your knowledge and learn about crucial aspects of cellular biology in an interactive format.

0 views • 50 slides


An Overview of Microscopes and Their Applications

Microscopes play a crucial role in clinical practice, with light microscopes being commonly used for viewing specimens. Different types of light microscopy, including brightfield and darkfield, offer unique ways to visualize organisms. Advanced techniques like phase-contrast and fluorescence microsc

0 views • 22 slides


Understanding Light Interactions: Reflection, Absorption, and Fluorescence

Explore the fascinating world of light interactions, where light can be reflected, absorbed, or transmitted based on the nature of the object it encounters. Learn about absorption in different materials, fluorescence, phosphorescence, and the concepts of specular and diffuse reflection.

0 views • 17 slides


Rapid Analysis of Soil Macronutrients Using Energy Dispersive X-ray Fluorescence

Determination of trace elements and macronutrients in agricultural soils is crucial for crop productivity. This study highlights the advantages, challenges, and objectives of using energy dispersive X-ray fluorescence for rapid and precise analysis. The method requires minimal sample preparation, co

0 views • 10 slides


Understanding the Staining Process in Microscopy

Staining is a vital technique in microscopy to enhance contrast and highlight structures in biological samples. Dyes and stains, with their specific chemical properties, play a key role in this process. The classification of dyes into acidic, basic, and neutral categories based on their chemical beh

0 views • 17 slides


Introduction to Microscopy: Care, Terms, and Operation

Explore the world of microscopy with proper care tips, essential terms like magnification and resolution, and detailed operation steps for using a microscope effectively. Learn about the components of a microscope and the process of preparing and viewing specimens at different magnification levels.

0 views • 27 slides


Introduction to Light Microscopy: Structure, Function, and Operation

Light microscopy is a vital instrument used for studying small organisms or particles invisible to the naked eye. This lab exercise delves into the history, types, components, and operation of light microscopes, focusing on human cheek cells and onion cells. Explore the magnification and resolution

0 views • 17 slides


Understanding Microscopy and Micrometry in Veterinary Microbiology

Explore the world of microscopy and micrometry with Dr. Sudha Kumari, Assistant Professor at Bihar Animal Sciences University. Learn about the history, types of microscopes, variables used in microscopy, and the significance of these instruments in observing microorganisms and their structures.

0 views • 15 slides


Revolutionary Incubation Systems for Microscopy Platforms

Our company specializes in cutting-edge incubation systems for microscopy platforms, offering innovative solutions like the world's first vibration-free system and a wide range of gas control options. Our products cater to major universities and research institutes, providing temperature stability d

0 views • 16 slides


Exploring Atomic Dimensions: Scanning Probe Microscopy

Delve into the world of nanoscale imaging with Scanning Probe Microscopy (SPM) techniques like Scanning Tunneling Microscopy (STM) and Atomic Force Microscope (AFM). Unlike optical microscopes, SPM methods break the diffraction limit by relying on intermolecular forces and quantum tunneling for unpa

0 views • 26 slides


Exploring Confocal Microscopy: Adding the Third Dimension

Delve into the world of confocal microscopy with Lecture 07 of Principles of Modern Microscopy. Learn about optical sectioning, wide-field imaging, confocal laser scanning, and more to enhance fluorescence in microscopy. Understand the methods of optical sectioning such as deconvolution, multi-photo

0 views • 65 slides


Fundamentals of Microscopy in Microbiology: An Insightful Journey

Explore the intriguing world of microscopy in microbiology, delving into the history, magnification, resolution, numerical aperture, and focal length. Uncover the evolution of microscopes and the fundamental concepts that underpin the science of microscopy. Gain a deeper understanding of how these t

0 views • 14 slides


Understanding Transmission Electron Microscopy (TEM)

Transmission Electron Microscopy (TEM) is a powerful microscopy technique that uses a beam of electrons to create high-resolution images of ultra-thin specimens. It provides detailed information on topography, morphology, composition, and crystalline structure. Sample preparation for TEM is crucial,

0 views • 10 slides


Microscopic Techniques in Cell Biology: Lessons and Labs Overview

Explore the fascinating world of optical microscopy in cellular biology, covering topics such as image formation, contrasting techniques, fluorescence microscopy, live-imaging techniques, and quantitative microscopy. Dive into the principles behind various microscopy techniques and experimental labs

0 views • 7 slides