Laboratory Diagnosis of Malaria: Methods and Procedures
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PRACTICAL
ON BLOOD
PARASITES
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The two methods common in use :
1: Light microscopy
2: Rapid diagnostic tests (RDTs).
Common methods for parasitological diagnosis of
malaria
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RDT procedure
RDT procedure
RDT procedure
Laboratory diagnosis of malaria
Light microscopy:1: Preparing blood film
Light microscopy:1: Preparing blood film
Laboratory diagnosis of malaria
Light microscopy: Thick and thin films
Laboratory diagnosis of malaria
Plasmodium falciparum
(trophozoite
stage in thick smear)
Plasmodium falciparum
(trophozoite
stage in thin smear)
Laboratory diagnosis of malaria
16 Morphology of Malaria
3. Gametocyte
The Malaria Parasite
Three developmental
stages seen in blood
films:
1.
Trophozoite
2. Schizont
17 Morphology of Malaria
Species of malaria is identified by its
chracteristic microscopic
appearance
:
18 Morphology of Malaria
Plasmodium falciparum
(trophozoite stage in thin smear)
19 Morphology of Malaria
Plasmodium falciparum
(trophozoite stage in thick smear)
20 Morphology of Malaria
Plasmodium falciparum
(characteristic banana-shaped or crescent –
shaped gametocyte stage in thin smear)
A 25 year-old male from India,
who came 3 months ago was admitted in
KKUH with a history of severe anaemia and intermittent high grade fever
for the last two months not responding to antibiotics.
WHAT IS THE DIAGNOSIS?
Diagnosis: malaria or Plasmodium vivax
A businessman who makes frequent trips to Thailand , presents with
intermittent fever
.
WHAT IS THE DIAGNOSIS?
Diagnosis: malaria or Plasmodium vivax
A student in KSU who returned three weeks from vacation in Africa , he
developed intermittent fever last week and lost consciousness a short
time ago
.
WHAT IS THE DIAGNOSIS?
Diagnosis: malaria or Plasmodium falciparum
The patient was then treated with schizontocidal antimalarial drugs, a
follow-up blood film is shown
.
ARE THERE ANY PARASITES? WHAT STAGE ?
Plasmodium flaciparum , gametocyte stage
A 7 year old child presented with anemia , hepatospenomegaly and
fever .Not responding to antimalarials and antibiotics
. Bone marrow
smear is shown
ARE THERE ANY PARASITES? WHAT STAGE ?
Leishmania , amastigote stage
Bone marrow aspiration
Bone marrow amastigotes
Methods for diagnosing malaria in laboratory settings involve techniques such as light microscopy and rapid diagnostic tests. Light microscopy is used to prepare blood films, enabling the detection of malaria parasites like Plasmodium falciparum. Rapid diagnostic tests can quickly identify malaria antigens. Understanding the morphology of malaria parasites, including trophozoites, schizonts, and gametocytes, is crucial for accurate diagnosis.
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Presentation Transcript
PRACTICAL PRACTICAL ON BLOOD ON BLOOD PARASITES PARASITES
Common methods for parasitological diagnosis of malaria The two methods common in use : 1: Light microscopy 2: Rapid diagnostic tests (RDTs).
Laboratory diagnosis of malaria Laboratory diagnosis of malaria Rapid diagnostic tests detect Rapid diagnostic tests detect malaria antigens malaria antigens
Laboratory diagnosis of malaria Light microscopy:1: Preparing blood film
Light microscopy:1: Preparing blood film Laboratory diagnosis of malaria
Laboratory diagnosis of malaria Light microscopy: Thick and thin films
Laboratory diagnosis of malaria Plasmodium falciparum (trophozoite stage in thick smear) Plasmodium falciparum (trophozoite stage in thin smear) CCMOV BD
16 Morphology of Malaria The Malaria Parasite Trophozoites Three developmental stages seen in blood films: CCMOVBD CCMOVBD 1. Trophozoite Schizont Gametocyte 2. Schizont 3. Gametocyte CCMOVBD CCMOVBD
17 Morphology of Malaria Plasmodium vivax CCMOVBD Species of malaria is identified by its chracteristic microscopic appearance: Plasmodium ovale Plasmodium malariae Malaria Tutorials, Wellcome Trust CCMOVBD
18 Morphology of Malaria Plasmodium falciparum (trophozoite stage in thin smear) CCMOVBD
19 Morphology of Malaria Plasmodium falciparum (trophozoite stage in thick smear) CCMOVBD
20 Morphology of Malaria Plasmodium falciparum (characteristic banana-shaped or crescent shaped gametocyte stage in thin smear) CCMOVBD
A 25 year-old male from India, who came 3 months ago was admitted in KKUH with a history of severe anaemia and intermittent high grade fever for the last two months not responding to antibiotics. WHAT IS THE DIAGNOSIS? Diagnosis: malaria or Plasmodium vivax
A businessman who makes frequent trips to Thailand , presents with intermittent fever . WHAT IS THE DIAGNOSIS? Diagnosis: malaria or Plasmodium vivax
A student in KSU who returned three weeks from vacation in Africa , he developed intermittent fever last week and lost consciousness a short time ago. WHAT IS THE DIAGNOSIS? Diagnosis: malaria or Plasmodium falciparum
The patient was then treated with schizontocidal antimalarial drugs, a follow-up blood film is shown . ARE THERE ANY PARASITES? WHAT STAGE ? Plasmodium flaciparum , gametocyte stage
A 7 year old child presented with anemia , hepatospenomegaly and fever .Not responding to antimalarials and antibiotics . Bone marrow smear is shown ARE THERE ANY PARASITES? WHAT STAGE ? Leishmania , amastigote stage
Bone marrow aspiration Bone marrow amastigotes
