Explore the practical steps involved in analyzing single-cell RNA sequencing (scRNA-seq) data with Seurat in this informative session. Learn how to install R and essential packages, download example datasets, understand raw data files, load data into R-Studio, and access data within a Seurat object.

3 views • 19 slides

Gene translation from RNA to protein involves the specific binding of transfer RNA molecules to messenger RNA codons, enabling the translation of nucleotide sequences into amino acids. Each amino acid is attached to a specific tRNA through activating enzymes, and the anticodon on tRNA pairs with the

0 views • 4 slides

Delve into the Genetics and Genome Evolution (GGE) Bioinformatics for Genomics Lecture Series 2022 presented by Sven Bergmann. Explore topics like RNA-seq, differential expression analysis, clustering, gene expression data analysis, epigenetic data analysis, integrative analysis, CHIP-seq, HiC data,

0 views • 36 slides

This content provides information on the Summer Institutes of Statistical Genetics module, focusing on gene expression profiling. It includes details on the schedule, experimental design, RNA sequencing workflow, modes of bulk RNA sequencing, and RNASeq software. The content discusses crucial steps

0 views • 11 slides

This content delves into the intricacies of analyzing 10X single-cell RNA-Seq data, covering topics such as how 10X RNA-Seq works, evaluating CellRanger QC reports, dimensionality reduction techniques like PCA, tSNE, and UMAP, using the Loupe cell browser, and frameworks for scRNA analysis in R. It

0 views • 39 slides

Explore the intricacies of analyzing 10X Single-Cell RNA-Seq data, from how the technology works to using tools like CellRanger, Loupe Cell Browser, and Seurat in R. Learn about the process of generating barcode counts, mapping, filtering, quality control, and quantitation of libraries. Dive into di

0 views • 34 slides

Delve into the world of nucleic acids with a focus on DNA and RNA molecules. Discover the fundamental building blocks, such as nucleotides, and the role they play in genetic information storage and cell function. Learn about the unique characteristics of DNA like its double-helix structure and the s

0 views • 26 slides

A primary transcript is the initial RNA copy of a transcription unit, subject to posttranscriptional modifications like cleavage and further alterations to form functional tRNAs, rRNAs, and mRNAs. In eukaryotic cells, pre-rRNAs and pre-tRNAs undergo processing by ribonucleases to yield mature RNA sp

0 views • 12 slides

A research project at Bowling Green State University aims to identify 3D motifs in RNA hairpin and internal loops using sequence and secondary structure information. The study focuses on finding likely sequence variants of known motifs, leveraging geometric considerations and basepair isostericity f

0 views • 28 slides

Tophat2 is a versatile pipeline aimed at aligning RNA reads to a genome, addressing gaps in alignments through innovative strategies. It involves the use of Bowtie for mapping and is written in C++, with a Python wrapper for seamless execution. The pipeline deals with large datasets and requires var

0 views • 13 slides

Dive into the world of RNA-Seq expression analysis through topics such as read mapping, quantitation, and the challenges faced in short-read mapping. Discover the typical parameters, design pressures, and basic ideas behind suffix tree index construction for efficient handling of large reads and dat

0 views • 19 slides

Workshop focused on analyzing circulating miRNA changes associated with Alzheimer's and Parkinson's diseases using the Genboree Workbench and exceRpt small RNA-seq analysis pipeline. The study aimed to identify extracellular miRNA biomarkers correlating with disease status and progression, replicati

0 views • 20 slides

Protein structure is composed of amino acids arranged in specific orders to form polypeptides. This process involves transcription of DNA into RNA followed by translation of RNA into proteins. Replication plays a crucial role in preparing DNA for cell division. The central dogma of molecular biology

0 views • 24 slides

DNA transcription is the process where DNA is used as a template to create mRNA in the nucleus. This mRNA is complementary to the DNA and goes through initiation, elongation, and termination stages. RNA polymerases are essential for this process in eukaryotes. Various RNA polymerases have specific f

0 views • 47 slides

Gene expression involves two crucial phases - transcription and translation. Transcription, the first step, is the process where RNA is synthesized from DNA with the help of RNA polymerase. It begins with initiation, where the enzyme recognizes the promoter region and forms a complex with the DNA, m

0 views • 33 slides

Transcription is a crucial process where RNA is synthesized from DNA, involving complex steps of initiation, elongation, and termination. This process is regulated by specific DNA regions, proteins, and enzymes like DNA-dependent RNA polymerase. The differences between DNA and RNA synthesis lie in t

0 views • 15 slides

The process of RNA transcription involves three main stages: initiation, elongation, and termination. Initiation starts with RNA polymerase binding to a promoter, followed by the formation of a transcription initiation complex. Elongation involves RNA polymerase untwisting the DNA helix and adding n

0 views • 17 slides

Explore the intricate processes of transcription and RNA processing in molecular biology, delving into the types of RNA molecules, the differences between DNA and RNA, RNA polymerase enzymes, transcription factors, and the preinitiation complex. Visual aids and detailed descriptions help elucidate t

0 views • 55 slides

RNA polymerases play a crucial role in synthesizing cellular RNA through transcription, where RNA is created from a DNA template. This process involves specific requirements such as a DNA template, ribonucleoside triphosphates, and divalent metal ions. RNA polymerase catalyzes the initiation and elo

0 views • 15 slides

Oysters, as high-yield mariculture varieties, were investigated for novel RNA viruses using virome analysis. Eighteen new RNA viruses were identified, with evidence of genetic recombination. This study highlights the importance of advanced sequencing technologies in discovering viral pathogens in aq

0 views • 11 slides

Analysis of ChIP-seq data in primate iPSCs reveals insights into regulatory differences, experimental systems, read subsampling, QC analysis, peak classification, and cross-species comparisons for transcriptional regulation studies. Balanced designs and functional validation of iPSCs contribute to a

0 views • 33 slides

RNA is a common component of the human diet, with naturally occurring RNAi mechanisms in various organisms. While staple foods contain RNA, barriers in the human body limit systemic exposure, making oral ingestion ineffective for RNAi. Studies have shown minimal bioavailability and efficacy of dieta

0 views • 9 slides

Explore RNA secondary structure prediction methods and results, highlighting the role of tRNA and rRNA in cellular RNA composition. The process involves cycling through sequence folds, scoring based on base alignments, and identifying cis-regulatory elements. Results showcase paired bases in tRNA an

0 views • 7 slides

RNA secondary structure prediction is a key concept in bioinformatics, encompassing features like stems, loops, and bulges. This presentation delves into the importance of RNA beyond mRNA, highlighting rRNA, tRNA, and regulatory RNA roles. The canonical base pairs A-U and C-G shape the single-strand

0 views • 20 slides

Nucleic acids are linear polymers composed of nucleotides, with DNA and RNA being the main types. RNA consists of messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA), each playing a crucial role in protein synthesis. mRNA carries genetic information from DNA to ribosomes, tRNA transf

0 views • 8 slides

Nucleic acids are essential molecules that store genetic information and aid in protein production. This content delves into the monomeric structure of nucleotides, the differences between DNA and RNA, the unique characteristics of DNA's double helix structure versus RNA's single-stranded form, and

0 views • 11 slides

Research spanning over two decades has delved deep into the marine RNA virosphere, shedding light on the complex marine ecosystem and the characteristics of RNA viruses. Discoveries in deep marine virus taxonomy have led to significant taxonomic changes and advancements in virus classification and g

0 views • 10 slides

Exploring a comprehensive guide to RNA-Seq and transcriptome analysis presented in a PowerPoint by Shounak Bhogale. The tutorial covers steps such as aligning RNA-Seq reads using STAR, counting reads with htseqCounts, finding differentially expressed genes with edgeR, and visualization with IGV. Det

0 views • 30 slides

Bulk RNA-seq is a powerful method for analyzing gene expression in biological samples. This approach involves extracting and sequencing RNA to understand the presence and quantity of RNA molecules. The process includes steps like conversion to cDNA, sequencing reads, and downstream analysis over ann

0 views • 12 slides

Explore the application of limma-voom, a statistical method to analyze gene expression data from RNA-Seq experiments. Understand how this approach addresses the variance issue in RNA-Seq data by applying weights. Utilize bottomly data from inbred mouse strains to identify differentially expressed ge

0 views • 11 slides

Delve into the world of genomics and bioinformatics through the Genetics and Genome Evolution (GGE) lecture series by Sven Bergmann. Explore topics such as RNA-seq analysis, differential expression, gene expression measurement techniques, and integrative analysis with epigenetic data. Gain insights

0 views • 37 slides

Explore the intricate process of RNA-Seq analysis including library preparation, reference-based analysis, sequence data processing, raw sequence quality control, FastQ format data, and quality assessment steps such as FastQC, base call quality, composition, duplication, and adapter trimming.

0 views • 81 slides

Exploring RNA-Seq data analysis focusing on mapping reads and estimating transcript abundance. Key topics include read mapping speed, quantitation methods, inferences on gene expression, and a simple model for understanding transcript abundance calculation. The process involves linking mapped reads

0 views • 13 slides

Multiple enhancers play a crucial role in regulating gene expression in a cell, with approximately 3-5 enhancers associated per human gene. These enhancers are essential for tissue-specific expressions and are active within the same cell line. The use of multiple enhancers can impact gene expression

0 views • 30 slides

Ribonucleic acid (RNA) is a crucial polymeric molecule in various biological functions including coding, decoding, gene regulation, and expression. It works alongside DNA and plays a key role in protein synthesis. There are three major types of RNA - rRNA, tRNA, and mRNA, each with specific function

0 views • 5 slides

The content delves into the intricacies of DNA and RNA, highlighting the significance of DNA safety in the nucleus, the role of RNA in protein synthesis, different types of RNA, the transcription process, and the importance of RNA in genetic information transfer. It also discusses how DNA is transcr

0 views • 35 slides

Explore the fundamentals of DNA, RNA, and protein synthesis in this detailed review. Learn about the structure of DNA, the role of different nucleotides, DNA replication process, RNA structure, various types of RNA, transcription from DNA to RNA, and translation from RNA to protein. Dive into the wo

0 views • 16 slides

Explore essential strategies for analysing BS-Seq data, ranging from selecting appropriate methylation contexts to identifying coverage outliers and biases. Understand how to interpret data patterns, handle unusual findings, and mitigate potential errors, ensuring accurate methylation analysis resul

0 views • 40 slides

Selective precipitation of RNA using Lithium Chloride (LiCl) and extraction with phenol at acidic pH are two common methods for separating RNA from DNA. LiCl forms a complex with RNA facilitating its precipitation, while acidic phenol solution helps stabilize RNA at pH 4-5 for extraction. TRIzol var

0 views • 7 slides

Plant bioinformatics researchers conducted experimental analyses and mapped gene regulatory networks using ChIP-Seq data. The studies involved exploring gene expression, regulatory interactions, and transcription factor binding sites. Techniques such as peak calling, motif finding, and peak annotati

0 views • 19 slides