Proper Section Cutting Techniques for Microscopy
Learn about proper section cutting techniques for light and electron microscopy, including the ideal thickness for sections. Discover the essential steps for cutting paraffin sections and common faults to avoid. Find out the key points necessary for easy and successful sectioning, such as equipment requirements, knife preparation, setting considerations, tissue processing, technologist skill, cutting rate, and environmental factors.
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Presentation Transcript
PRACTICAL -5 PROPER SECTION CUTTING By: Khadija AlZahrani
PROPER SECTION CUTTING Typically 5 m thick for light microscopy And 80-100 nm thick for electron microscopy.
TECHNIQUES OF CUTTING PARAFFIN SECTIONS: 1- Orientation of the block on the microtome 2- Setting the knife in the suitable angle 3- Trimming or shaving the block 4- Cutting the section to the required thicken GENERAL FAULTS WHEN CUTTING PARAFFIN SECTIONS: a- Blunt knife and a damage knife edge b- Block or knife not well locked by screws or both c- Incorrect setting of the knife
For easy and good sectioning the following points are necessary: 1- Appropriate microtome and appropriate knife 2- Well prepared knife 3- Perfect setting includes: a-Relation of the knife to the object b-Tilt of inclination of the knife c-Angel clearance which depends on the profile of the knife, it govern the angel of slant. 4- Proper processed tissue 5- The skill of the technologist 6- The rate of cutting 7- The temperature of the atmosphere and the moisture 8- The orientation of the tissue block.
