Microscopes: Light vs. Electron Microscopes
Microscope
Light Microscope
Electron
Microscope
•
The Magnification power of the LM is limited to
1000-2000 X.
•
The Resolving power (resolution) of the LM is
0.2 µm.
•
The resolving power (resolution) is defined as the smallest
distance between two particles at which they can be seen as
separated objects.
•
Note: 1
µm
=
1e-6 m
Light Microscope (LM)
PARTS
1.
Eyepiece Lens
:
2.
Base:
3.
Stage:
4.
Revolving
Nosepiece or Turret:
5.
Objective Lenses:
6.
Diaphragm or Iris:
7.
Coarse adjustment
knob:
8.
Fine adjustment
knob:
•
The Magnification power of the EM is limited to
2,000,000 X.
•
The Resolving power (resolution) of the LM is
0.1 nm.
•
The resolving power (resolution) is defined as the smallest
distance between two particles at which they can be seen as
separated objects.
•
Note: 1
nm
=
1e-9 m
Electron Microscope (EM)
Types:
1.
Transmission Electron Microscope (TEM)
2.
Scanning Electron Microscope (SEM)
Learn about the differences between light microscopes (LM) and electron microscopes (EM), including their magnification power, resolving power, and key parts. Explore the types of electron microscopes such as Transmission Electron Microscope (TEM) and Scanning Electron Microscope (SEM) for advanced imaging capabilities.
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Presentation Transcript
Microscope Light Microscope ElectronMicroscope
Light Microscope (LM) The Magnification power of the LM is limited to 1000-2000 X. The Resolving power (resolution) of the LM is 0.2 m. The resolving power (resolution) is defined as the smallest distance between two particles at which they can be seen as separated objects. Note: 1 m = 1e-6 m
PARTS 1. 2. 3. 4. Eyepiece Lens: Base: Stage: Revolving Nosepiece or Turret: Objective Lenses: Diaphragm or Iris: Coarse adjustment knob: Fine adjustment knob: 5. 6. 7. 8.
Electron Microscope (EM) The Magnification power of the EM is limited to 2,000,000 X. The Resolving power (resolution) of the LM is 0.1 nm. The resolving power (resolution) is defined as the smallest distance between two particles at which they can be seen as separated objects. Note: 1nm = 1e-9 m
Types: 1. Transmission Electron Microscope (TEM) 2. Scanning Electron Microscope (SEM)
