Vi-CELL.BLU is a cutting-edge cell counting instrument that offers faster analysis, increased resolution, and improved optical sensor technology for enhanced cell concentration and viability assessments. The device features a user-friendly interface, Trypan blue method for live cell detection, and a

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Viruses interact with host cells in various ways, encoding genes that manipulate cell functions for their benefit. These interactions can range from benign to lethal outcomes. Factors influencing these interactions include viral factors, cellular responses, and the presence of virulence factors. Dif

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Explore the practical steps involved in analyzing single-cell RNA sequencing (scRNA-seq) data with Seurat in this informative session. Learn how to install R and essential packages, download example datasets, understand raw data files, load data into R-Studio, and access data within a Seurat object.

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Gene translation from RNA to protein involves the specific binding of transfer RNA molecules to messenger RNA codons, enabling the translation of nucleotide sequences into amino acids. Each amino acid is attached to a specific tRNA through activating enzymes, and the anticodon on tRNA pairs with the

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Delve into the Genetics and Genome Evolution (GGE) Bioinformatics for Genomics Lecture Series 2022 presented by Sven Bergmann. Explore topics like RNA-seq, differential expression analysis, clustering, gene expression data analysis, epigenetic data analysis, integrative analysis, CHIP-seq, HiC data,

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This content delves into the intricacies of analyzing 10X single-cell RNA-Seq data, covering topics such as how 10X RNA-Seq works, evaluating CellRanger QC reports, dimensionality reduction techniques like PCA, tSNE, and UMAP, using the Loupe cell browser, and frameworks for scRNA analysis in R. It

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Explore the intricacies of analyzing 10X Single-Cell RNA-Seq data, from how the technology works to using tools like CellRanger, Loupe Cell Browser, and Seurat in R. Learn about the process of generating barcode counts, mapping, filtering, quality control, and quantitation of libraries. Dive into di

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A primary transcript is the initial RNA copy of a transcription unit, subject to posttranscriptional modifications like cleavage and further alterations to form functional tRNAs, rRNAs, and mRNAs. In eukaryotic cells, pre-rRNAs and pre-tRNAs undergo processing by ribonucleases to yield mature RNA sp

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A research project at Bowling Green State University aims to identify 3D motifs in RNA hairpin and internal loops using sequence and secondary structure information. The study focuses on finding likely sequence variants of known motifs, leveraging geometric considerations and basepair isostericity f

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Cell division plays a crucial role in the growth and reproduction of all organisms. In prokaryotic cells, binary fission is the primary mode of division, while eukaryotic cells undergo a more complex process involving cell growth, DNA replication, chromosome distribution, and cytokinesis. The cell c

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Dive into the world of RNA-Seq expression analysis through topics such as read mapping, quantitation, and the challenges faced in short-read mapping. Discover the typical parameters, design pressures, and basic ideas behind suffix tree index construction for efficient handling of large reads and dat

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Protein structure is composed of amino acids arranged in specific orders to form polypeptides. This process involves transcription of DNA into RNA followed by translation of RNA into proteins. Replication plays a crucial role in preparing DNA for cell division. The central dogma of molecular biology

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DNA transcription is the process where DNA is used as a template to create mRNA in the nucleus. This mRNA is complementary to the DNA and goes through initiation, elongation, and termination stages. RNA polymerases are essential for this process in eukaryotes. Various RNA polymerases have specific f

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Transcription is a crucial process where RNA is synthesized from DNA, involving complex steps of initiation, elongation, and termination. This process is regulated by specific DNA regions, proteins, and enzymes like DNA-dependent RNA polymerase. The differences between DNA and RNA synthesis lie in t

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The process of RNA transcription involves three main stages: initiation, elongation, and termination. Initiation starts with RNA polymerase binding to a promoter, followed by the formation of a transcription initiation complex. Elongation involves RNA polymerase untwisting the DNA helix and adding n

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Explore the intricate processes of transcription and RNA processing in molecular biology, delving into the types of RNA molecules, the differences between DNA and RNA, RNA polymerase enzymes, transcription factors, and the preinitiation complex. Visual aids and detailed descriptions help elucidate t

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RNA polymerases play a crucial role in synthesizing cellular RNA through transcription, where RNA is created from a DNA template. This process involves specific requirements such as a DNA template, ribonucleoside triphosphates, and divalent metal ions. RNA polymerase catalyzes the initiation and elo

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The cell cycle plays a crucial role in the cellular uptake and dilution of nanoparticles within a cell population. This process involves different phases such as G1, S, G2, and M, each with specific functions related to cell growth, DNA synthesis, protein synthesis, and cell division. Understanding

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RNA is a common component of the human diet, with naturally occurring RNAi mechanisms in various organisms. While staple foods contain RNA, barriers in the human body limit systemic exposure, making oral ingestion ineffective for RNAi. Studies have shown minimal bioavailability and efficacy of dieta

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Explore RNA secondary structure prediction methods and results, highlighting the role of tRNA and rRNA in cellular RNA composition. The process involves cycling through sequence folds, scoring based on base alignments, and identifying cis-regulatory elements. Results showcase paired bases in tRNA an

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RNA secondary structure prediction is a key concept in bioinformatics, encompassing features like stems, loops, and bulges. This presentation delves into the importance of RNA beyond mRNA, highlighting rRNA, tRNA, and regulatory RNA roles. The canonical base pairs A-U and C-G shape the single-strand

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Nucleic acids are linear polymers composed of nucleotides, with DNA and RNA being the main types. RNA consists of messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA), each playing a crucial role in protein synthesis. mRNA carries genetic information from DNA to ribosomes, tRNA transf

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Complementary nitrogenous bases in DNA, pyrimidines (thymine, cytosine) and purines (adenine, guanine), store biological information through antiparallel strands. DNA, with non-coding regions, undergoes replication and transcription to RNA for protein synthesis. Nucleic acids, DNA, and RNA are cruci

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Nucleic acids are essential molecules that store genetic information and aid in protein production. This content delves into the monomeric structure of nucleotides, the differences between DNA and RNA, the unique characteristics of DNA's double helix structure versus RNA's single-stranded form, and

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Research spanning over two decades has delved deep into the marine RNA virosphere, shedding light on the complex marine ecosystem and the characteristics of RNA viruses. Discoveries in deep marine virus taxonomy have led to significant taxonomic changes and advancements in virus classification and g

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Exploring a comprehensive guide to RNA-Seq and transcriptome analysis presented in a PowerPoint by Shounak Bhogale. The tutorial covers steps such as aligning RNA-Seq reads using STAR, counting reads with htseqCounts, finding differentially expressed genes with edgeR, and visualization with IGV. Det

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Explore the intricate world of cell biology through this comprehensive guide covering the definition of a cell, the characteristics of animal cells, the role of the nucleus, ribosomes, cell structure, organelles, cell membrane, and cell wall. Discover the fascinating details of eukaryotic cells and

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Explore the vital functions of different cell organelles such as the cell membrane, nucleus, endoplasmic reticulum, lysosomes, Golgi apparatus, mitochondria, cytoplasm, ribosomes, vacuole, cell wall, chloroplasts, and chlorophyll in a cell. Learn how each organelle plays a unique role in maintaining

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Bulk RNA-seq is a powerful method for analyzing gene expression in biological samples. This approach involves extracting and sequencing RNA to understand the presence and quantity of RNA molecules. The process includes steps like conversion to cDNA, sequencing reads, and downstream analysis over ann

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Explore the application of limma-voom, a statistical method to analyze gene expression data from RNA-Seq experiments. Understand how this approach addresses the variance issue in RNA-Seq data by applying weights. Utilize bottomly data from inbred mouse strains to identify differentially expressed ge

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Delve into the world of genomics and bioinformatics through the Genetics and Genome Evolution (GGE) lecture series by Sven Bergmann. Explore topics such as RNA-seq analysis, differential expression, gene expression measurement techniques, and integrative analysis with epigenetic data. Gain insights

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Explore the intricate process of RNA-Seq analysis including library preparation, reference-based analysis, sequence data processing, raw sequence quality control, FastQ format data, and quality assessment steps such as FastQC, base call quality, composition, duplication, and adapter trimming.

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Exploring RNA-Seq data analysis focusing on mapping reads and estimating transcript abundance. Key topics include read mapping speed, quantitation methods, inferences on gene expression, and a simple model for understanding transcript abundance calculation. The process involves linking mapped reads

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Multiple enhancers play a crucial role in regulating gene expression in a cell, with approximately 3-5 enhancers associated per human gene. These enhancers are essential for tissue-specific expressions and are active within the same cell line. The use of multiple enhancers can impact gene expression

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Ribonucleic acid (RNA) is a crucial polymeric molecule in various biological functions including coding, decoding, gene regulation, and expression. It works alongside DNA and plays a key role in protein synthesis. There are three major types of RNA - rRNA, tRNA, and mRNA, each with specific function

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The content delves into the intricacies of DNA and RNA, highlighting the significance of DNA safety in the nucleus, the role of RNA in protein synthesis, different types of RNA, the transcription process, and the importance of RNA in genetic information transfer. It also discusses how DNA is transcr

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Animal tissue culture involves growing tissues separate from the animal in a laboratory setting. To achieve exponential cell growth, cells are converted into immortal cell lines. The production of a cell line involves steps like breaking cell adhesion, incubation, and transferring cells to fresh med

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Explore the fundamentals of DNA, RNA, and protein synthesis in this detailed review. Learn about the structure of DNA, the role of different nucleotides, DNA replication process, RNA structure, various types of RNA, transcription from DNA to RNA, and translation from RNA to protein. Dive into the wo

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Selective precipitation of RNA using Lithium Chloride (LiCl) and extraction with phenol at acidic pH are two common methods for separating RNA from DNA. LiCl forms a complex with RNA facilitating its precipitation, while acidic phenol solution helps stabilize RNA at pH 4-5 for extraction. TRIzol var

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Nucleic acids, including DNA and RNA, are essential molecules storing genetic information for cellular functions. Comprised of nucleotides with nitrogenous bases, sugars, and phosphate groups, they play crucial roles in intracellular signaling, energy transfer, and genetic makeup determination. Ribo

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