Vi-CELL.BLU - Advanced Cell Counting Instrument

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Vi-CELL.BLU is a cutting-edge cell counting instrument that offers faster analysis, increased resolution, and improved optical sensor technology for enhanced cell concentration and viability assessments. The device features a user-friendly interface, Trypan blue method for live cell detection, and a rich history of cell counting methodologies. With advantages like rapid analysis, minimal sample volume requirements, and comprehensive automation, Vi-CELL.BLU is at the forefront of cell analysis technology.

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Vi-CELL BLU INCREASE YOUR Vi-LOCITY 2019-FM-PCC-UKI-02

Vi-CELL BLU Cell concentration and viability Increase your vi-locity

Vi-CELL BLU History of Cell Counting Electrical Sensing Zone Principle Coulter Counting Coulter Model A 1953 ZM 1982 Z2 Multisizer 4e 2016 1994 Image Based Cell Analyzer Vi-CELL XR 2003 Vi-CELL BLU 2019

Vi-CELL BLU Introduction Advantages of the Vi-CELL BLU: Faster analysis: 130 sec Less volume: 200 l SOP with fully automated routines incl. cleaning 24 position carousel 96 well plate loader Win 10 OS integrated computer Touchscreen interface

Vi-CELL BLU Trypan blue method Principle: Live cells with intact cell membranes exclude dyes like trypan blue cells look translucent green circle dead cells allow the dye to penetrate through their damaged membrane cells look dark red circle

Vi-CELL BLU Instrument

Vi-CELL BLU Instrument

Vi-CELL BLU Optical sensor Vi-CELL BLU Vi-CELL XR The larger sensor size of the Vi-CELL BLU and optical configuration results in approximately 1.7 fold greater imaged area resulting in more objects captured per image. Coupled with the faster acquisition speed of the camera it is possible to image 100 frames in less time that required by the Vi-CELL XR. This results in increased statistical confidence in the results.

Vi-CELL BLU Resolution Vi-CELL BLU Vi-CELL XR While the physical size of the images from the Vi-CELL BLU are only 1.7x larger they pack almost twice as many pixels into that space. This higher pixel density produces a much smoother gradation of object edges making for a more accurate segmentation of object boundaries and is particularly advantageous for declustering operations.

Vi-CELL BLU Interface

Vi-CELL BLU Interface

Vi-CELL BLU Sample Volume Volume of Cell Culture Used in Measurement over X Days 12 Cell Culture Volume (mL) 10 8 6 XR 4 BLU 2 0.60 mL sample vial 2.5mL sample vial 0 1 3 6 9 12 15 18 21 Day Day Day Day Day Day Day Day

Vi-CELL BLU Easier to Use and Greater Traceability 310 activities per kit RFID tag keeps track of: Lot number Expiration date On-board life (90 days) Activities remaining

Vi-CELL BLU Flexibility: Changing from carousel to plate

Vi-CELL BLU Single Use Controls Concentration 0,5x10^6 (0,5 M) 2x10^6 (2M) 4x10^6 (4M) 1x10^7 (10M) Viability 50% Viability Mixture of small and large beads. Size 10 micron NIST size beads Focus monitoring

Vi-CELL BLU Data Integrity 21 CFR Part 11 Compliance Audit Trails Error Log System Activity Log User Login User Levels Electronic Signatures Exporting/Backup/Clean-up

Vi-CELL BLU Cell Type Cell type Minimum threshold for detected cells (lowest 2 m) Maximum threshold for detected cells (highest 60 m) Number of images (recommended 100) Defines the sharpness of the separation of the cell from the background. Can be used to reject debris (least circular=0, perfectly circular =1) Increases the ability to detect cells that are clumped together. Default setting is Medium. Number of resuspension cycles. Default setting is 3. Defines the brightness degree from which a cell is defined as a vital cell. 55% is a typical value. Defines the size of the inner bright area defining a vital cell. 5 10% are typical values Number of mixing cycles with trypan blue and cell suspension.