Enzyme kinetics - PowerPoint PPT Presentation


Enzymes are Proteins that act as Biological Catalyst

Enzymes, as proteins acting as biological catalysts, accelerate chemical reactions without being consumed. They have active sites where substrates bind, and their specificity can be lock-and-key or induced fit. Enzymes are named based on their function or the substrates they interact with. Enzyme sp

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Aspen Simulation of Steam Reforming and Haber-Bosch Processes in Kinetics Reactors

Aspen simulation showcases the kinetics reactors for steam reforming of natural gas and the Haber-Bosch process for ammonia production. Steam reforming is highly endothermic, producing hydrogen and CO, while the Haber-Bosch process is exothermic, crucial for ammonia synthesis. The RPlug reactor and

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Understanding the Role of Phosphatase Enzymes in Metabolic Reactions

Phosphatase enzymes play a crucial role in various metabolic processes by releasing phosphate groups, increasing their availability for energy synthesis and cell structure formation. Acid phosphatases, with an optimum pH below 7.0, can be extracted from plant tissues like germinating mung beans. A p

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Understanding Enzyme Function Through Induced Fit Model

The learning content discusses enzymes as biological catalysts that increase reaction rates by lowering activation energy. It explores how enzyme activity efficiency is influenced by factors such as temperature, pH, and substrate concentration. The Induced Fit Model is analyzed to explain how enzyme

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Phylogenetic Analysis of Enzyme Sequences in Plant Species

The content discusses the amino acid sequence alignment and phylogenetic tree analysis of enzyme sequences, specifically DcLCYB2, among different plant species including Daucus carota, Carica papaya, Citrus maxima, Solanum lycopersicum, and Crocus sativus. The study explores the genetic relationship

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Chemical Kinetics: Understanding Reaction Rates and Factors

Chemical kinetics is a branch of physical chemistry that explores the velocity and factors influencing chemical reactions. It studies how reactants transform into products, considering conditions like temperature, pressure, and reactant concentrations. Factors affecting reaction rates include the na

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Understanding Bioprocessing: Advantages, History, Applications, and Enzyme Use

Bioprocessing utilizes biological materials for commercial, medical, and scientific purposes, leveraging natural biological activities to develop useful products. This branch of biotechnology offers advantages such as low temperature, pressure, and pH requirements, along with renewable resources for

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Investigating the Impact of Pineapple Enzyme Bromelain on Gelatin and Pasteurization Process

Explore the effects of pineapple enzyme bromelain on gelatin and how pasteurization can alter its effectiveness in this lab experiment. Discover the different outcomes when using fresh versus canned pineapple and formulate hypotheses to guide the investigation. Follow step-by-step procedures to test

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Factors Affecting Enzyme Activity and Catalysis

Enzyme activity is influenced by various factors such as enzyme concentration, temperature, pH, substrate concentration, inhibitors, activators, and physical agents. The rate of enzyme-catalyzed reactions is directly proportional to enzyme concentration, and temperature plays a significant role with

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Understanding Enzyme Function: The Key Steps and Importance of Cofactors

Enzymes play a crucial role in catalyzing biochemical reactions by forming enzyme-substrate complexes and facilitating changes in substrate molecules to product molecules. The process involves four steps: proximity of enzyme and substrate, binding at the active site, catalysis leading to substrate a

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Understanding Peroxidase Enzyme Activity in Biological Samples

Demonstrating the enzyme activity of peroxidase, an enzyme that plays a crucial role in breaking down hydrogen peroxide in various organisms. Learn about the differences between peroxidase and catalase, the calculation of enzyme activity, and the significance of extinction coefficient in enzyme assa

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Understanding Microbial Nutrition and Bacterial Physiology

Microbial nutrition involves essential elements required for microbial growth and energy production. Bacterial physiology delves into the structures and functions that enable bacteria to thrive, from cell wall composition to enzyme activities. Major elements like C, O, H, N, S, and P are crucial for

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Understanding Metabolic Control Mechanisms in Cellular Regulation

Metabolic control mechanisms play a crucial role in maintaining homeostasis within cells by regulating metabolic pathways. This involves finely adjusting the output of pathways in response to external signals, ensuring the proper flux of metabolites to meet cellular needs. Pacemaker enzymes, such as

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GCSE Required Practical Biology Experiments Overview

Explore GCSE Required Practical Biology experiments including using a light microscope, investigating osmosis, conducting food tests, and studying amylase enzyme activity. Understand the objectives, apparatus, results, and potential questions that may be asked in each experiment. Gain insights into

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Understanding Enzymes: Nature, Classification, and Mechanism of Action

Enzymes are biocatalysts synthesized by living cells. They are protein in nature and specific in their action. This article delves into the definition of enzymes, classification based on enzymatic action, enzyme class reactions, structure, and mechanism of enzyme action, highlighting how enzymes pla

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Exploring Enzyme Kinetics for Understanding Chemical Reactions

Enzyme kinetics is a vital discipline focusing on the rate of enzyme-catalyzed reactions and how they respond to varying conditions. Reactions are classified based on reactant concentration influences. Zero, first, second, and third order reactions are distinguished, with examples like first-order r

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Demonstration of Salivary Amylase Enzyme Action in B.Sc. Practical

Salivary amylase, an enzyme found in saliva, partially hydrolyzes starch into maltose. This practical involves observing the action of salivary amylase on starch, demonstrating how starch is broken down into glucose and maltose. The procedure includes preparing solutions, collecting saliva, mixing w

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Understanding Chemical Kinetics: Rates, Reactions, and Mechanisms

Chemical kinetics involves studying reaction rates, rate laws, stoichiometry, and factors affecting reaction speed. This branch of chemistry delves into determining reaction orders, rate constants, and activation energies using various methods. Different types of rates, such as initial, instantaneou

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Understanding the Kinetics of Fast Reactions in Chemistry

Kinetic methods involve measuring analytical signals under dynamic conditions to study fast reactions in chemistry. This study explores the various methods used, such as Flow Method and Stopped Flow Method, to determine reaction rates accurately. Advantages of the Stopped Flow Method over Continuous

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Understanding Kinetics and Reaction Rates in Chemistry

Kinetics is the study of reaction rates and factors affecting them, such as concentration, temperature, catalysts, and more. Orders of reaction classify reactions based on rate dependency on reactant concentration. Factors like pH, light, and solvents can also impact reaction rates. Half-life and sh

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Understanding Free Radical Polymerization Kinetics

This lecture covers the kinetics of free radical polymerization, including initiation, propagation, termination, and kinetic chain length concepts. It explains the calculation of kinetic chain length and chain-transfer reactions. Key points include the rate equations for initiation, propagation, and

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Enzyme Immobilization: Techniques and Applications in Industry and Healthcare

Enzyme immobilization involves confining enzymes on inert supports for stability and reuse, enhancing efficiency and cost-effectiveness. Historical events and examples illustrate various methods and applications. Chemical modifications, such as PEG addition, have shown increased enzyme activity. The

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Introduction to Kinematics and Dynamics of Machines in Mechanical Engineering

Theory of Mechanics delves into motion, time, and forces, with Kinematics focusing on motion analysis without considering external forces. Kinetics, a branch of Theory of Machines, deals with inertia forces resulting from mass and motion. Dynamics combines Kinematics and Kinetics to study motion and

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Factors Affecting Enzyme Activity and Kinetics Experiments

Explore the factors influencing enzyme activity, such as substrate and enzyme concentration, temperature, pH, and inhibitors. Learn how to simulate enzyme kinetics using equipment like popping beads and stopwatches. Analyze results to understand the impact of substrate concentration on reaction rate

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Understanding Enzyme Activity and Optimal Conditions

This interactive content provides a detailed exploration of enzyme activity through data interpretation and graph analysis. Questions range from identifying the impact of enzymes on specific molecules to determining optimal conditions for various enzyme functions such as pH and temperature. Users de

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Understanding Chemical Kinetics: Reaction Rates and Mechanisms

Chemical kinetics is a branch of chemistry focused on studying reaction rates and mechanisms. Unlike thermodynamics, which deals with feasibility, kinetics explores the speed at which reactions occur. Factors such as temperature, pressure, and catalysts influence reaction rates. Understanding the ra

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Immobilization of Enzymes in Biochemistry

Enzyme immobilization involves confining enzyme molecules to a distinct phase from substrates and products, attaching them to solid matrices for enhanced specificity and reduced inhibition. Inert polymers or inorganic materials are used as carrier matrices with methods like physical adsorption onto

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Understanding Enzyme Inhibition in Biochemistry

Enzyme inhibition plays a crucial role in pharmacology and biochemistry by regulating enzymatic reactions. Inhibitors can be reversible or irreversible, affecting enzyme activity differently. Competitive, uncompetitive, and noncompetitive inhibition types are explained along with examples like diiso

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Plant Enzyme Activities and Phylogenetic Analysis

The content discusses the alignment of various plant enzyme amino acid sequences, including DcLCYB1, DcLCYB2, and DcLCYE, among different inbred lines. It also explores the construction of DcLCYs for enzyme activities using an E. coli complementation assay. The phylogenetic relationship between DcLC

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Demonstration of Salivary Enzyme Amylase Action in B.Sc. Practical

Salivary enzyme amylase, also known as ptyalin, plays a crucial role in breaking down starch and glycogen into maltose. This practical session in the Zoology department explores the action of salivary enzyme amylase at a temperature of 37°C and pH of 6.6. By conducting experiments with starch, iodi

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Understanding Enzyme Inhibition in Acid Phosphatase Kinetics

In this experiment, the effect of inhibitors on enzymatic reactions, specifically acid phosphatase, is explored. Different types of inhibition (competitive, noncompetitive, uncompetitive) are examined, along with reversible and irreversible inhibitors. The study aims to determine the type of inhibit

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Reactor Sizing: Conversion, Selectivity, and Kinetics Overview

Understanding reactor design involves considerations such as desired conversion, selectivity, and kinetics. Key concepts include rate laws, molar balances, and reactor types. Through molar balance equations and reactor design processes, one can derive essential equations for ideal batch, CSTR, and P

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Chemical Kinetics and Numerical Solvers in Chemistry

Explore the principles of chemical kinetics and the use of numerical solvers to compute concentrations over time, considering explicit and implicit methods. Understand stability and positivity requirements in solvers and the importance of characteristic time scales in chemical systems. Dive into a s

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Fundamentals of Chemical Kinetics and Reactor Design

Explore the realm of chemical reactions, rate equations, and reactor design in this informative chapter. Understand the factors influencing reaction rates, different types of reactions, rate laws, and experimental determination of reaction rates. Dive into examples illustrating stoichiometry and rat

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Overview of Chemical Reactor Design and Operation

Chemical reactor design involves studying the rates and mechanisms of chemical reactions, as well as the design of reactors for these reactions on a commercial scale. This field combines principles from thermodynamics, chemical kinetics, fluid mechanics, mass transfer, heat transfer, and economics t

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Biology Review: Genetics, Enzymes, and Cell Division

Explore key concepts in AP Biology, including genetic continuity and change, enzyme catalysis, mitosis, and meiosis. Understand the mechanisms of genetic variability and cell division processes. Dive into enzyme-substrate interactions and the differences between mitosis and meiosis.

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Understanding the Interface to the Current CCPP: A Simple Model's Perspective

This content explores the interface to the current CCPP from a simple model's viewpoint, focusing on two simplistic schemes - kinetics and chem_solve. It delves into how the MusicBox simple driver model adapts chemical species using temperature and rate constants. Users can control the run sequence

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Understanding Chemical Kinetics and Equilibrium in Reactions

Explore the basic concepts of reaction rates, collision theory, activation energy, and energy diagrams in chemical kinetics and equilibrium. Learn how particles must collide with the correct orientation and enough energy to form an unstable activated complex. Discover the role of activation energy i

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Understanding ELISA Techniques in Cellular and Molecular Pharmacology

ELISA (Enzyme-Linked Immunosorbent Assay) is a vital immunological technique used in detecting antigens or antibodies in samples. This article covers the types of ELISA tests, such as Direct, Indirect, and Sandwich, along with the principles and applications of ELISA in diagnosis. The process involv

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Understanding Enzyme Assays in Biotechnology

Exploring enzyme assays in biotechnology, covering topics such as ELISA, protein assays, enzyme activity measurement techniques, specific activity, terminology related to enzyme assays, and different types of experiments conducted by biochemists to study enzyme-catalyzed reactions. The content delve

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