Genetic Engineering Techniques and Enzyme Palindromes
Explore the GGA method with pClone Red, EcoRI palindrome type II variations, and BsaI enzyme properties in genetic manipulation. Detailed images and descriptions provide insights into DNA cutting techniques and promoter sequences.
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GGA Method with pClone Red by A. Malcolm Campbell and Todd Eckdahl
Eco RI G CTTAA G AATTC palindrome type II
Eco RI G CTTAA G AATTC palindrome type II
Eco RI G CTTAA G AATTC type II
Eco RI G CTTAA AATTC G type II
Bsa I GAGACC CTCTGG not a palindrome type IIs
Bsa I 1234nGAGACC nCTCTGG ---- type IIs
Bsa I 1234nGAGACC nCTCTGG ---- type IIs
Bsa I GGTCTCn ---- CCAGAGn1234 type IIs
Bsa I GGTCTCn CCAGAGn1234 ---- type IIs
Bsa I 1234nGAGACC ----nCTCTGG GGTCTCn---- CCAGAGn1234 cuts left cuts right
Part J119137 BsaI cuts left BsaI cuts right
CGACtGAGACC GGTCTCa aCTCTGG CCAGAGtCGCC Bsa I GCGG GCTG Bsa I
CGACtGAGACC GGTCTCa aCTCTGG CCAGAGtCGCC GCGG GCTG
CGACtGAGACC GGTCTCa aCTCTGG CCAGAGtCGCC GCTG GCGG
CGACtGAGACC GGTCTCa aCTCTGG CCAGAGtCGCC GCGG GCTG CGAC(promoter) (promoter)CGCC
CGACtGAGACC GGTCTCa aCTCTGG CCAGAGtCGCC CGAC(promoter) GCGG GCTG (promoter)CGCC
GGA Ligation Method RBS GFP RBS RFP BsaI + Ligase origin antibiotic resistance plasmid backbone
GGA Ligation Method RBS GFP RBS RFP BsaI + Ligase origin antibiotic resistance plasmid backbone
