Analysis and Applications of Electron Microscopy in Science Education

Explore the in-depth analysis of quantitative and qualitative techniques in electron microscopy as it relates to interactions with matter, instrumentation, specimen preparation, elemental analysis, and more. Delve into the electromagnetic spectrum, radiation effects on the human body, and spectroscopic applications in microscopy. Understand the significance of microscopy in scientific research and education.

• Electron Microscopy
• Scientific Analysis
• Electromagnetic Spectrum
• Radiation Effects
• Spectroscopic Applications

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1. Quantitative And Qualitative Analysis In Electron Microscopy Prof. Dr. mer ANDA

2. DERS ER 1 Electromagnetic spectrum, Interaction of electromagnetic radiation with matter Elektron, De Broglie dalga boyu, B y tme oran dalga boyu ili kisi, Optik Mikroskop, 2

3. Electronspecimen interaction Instrumentation, Electron Emitters, Electron Lenses Specimen Preparation in Materials Science Procedure of sample preparation of a biological specimen Secondary electron imaging of topographic features Backscattered electron imaging of chemical phase difference Qualitative and quantitative elemental analysis with EDS Qualitative and quantitative elemental analysis with WDS Mid-Term Exam X-ray elemental reference standards for energy dispersive or wavelength dispersive X-ray microanalysis X-ray imaging: elemental line scans and maps SEM - vs - STEM (TEM) EBSD SEM Cathode-Luminescent Studies

4. ELECTROMAGNETIC SPECTRUM Radiowave Microwave IR UV Visible X-ray Gamma

5. Radiation and the Human Body INTERACTION OF ELECTROMAGNETIC RADIATION WITH HUMAN http://hyperphysics.phy-astr.gsu.edu/hbase/mod3.html

6. INTERACTION OF ELECTROMAGNETIC RADIATION WITH MATTER http://hyperphysics.phy-astr.gsu.edu/hbase/mod3.html

7. INTERACTION OF ELECTROMAGNETIC RADIATION WITH MATTER Spectroscopy: Interaction of light and matter All imaging systems map some spectroscopic property of the imaged object onto a detector. Nuclear Magnetic Resonance (NMR) spectroscopy Electron paramagnetic resonance (EPR) or electron spin resonance (ESR) spectroscopy Rotational Spectra : Microwave Spectroscopy Infrared spectroscopy (IR spectroscopy) Ultraviolet-Visible (UV-Vis) Spectroscopy X-ray fluorescence (XRF)spectroscopy X-ray photoelectron spectroscopy (XPS)

8. MICROSCOPY MICROSCOPY A microscope is an instrument that magnifies objects otherwise too small to be seen, producing an image in which the object appears larger Traditional Microscopes Optical Microscope; Scanning Electron Microscope (SEM); Transmission Electron Microscope (TEM);

9. Optical Microscope The optical microscope, often referred to as the light optical microscope, is a type of microscope that uses visible light and a system of lenses to magnify images of small samples The optical microscopy is a basic form of microscopic technology that depends on its analysis on the interaction between the light and matters

10. Magnification The numerical aperture of a microscope objective is the measure of its ability to gather light and to resolve fine specimen detail while working at a fixed object (or specimen) distance. Useful Magnification (total) = 500 to 1000 NA (Objective)

11. Numerical Aperture (NA) = sin()(1) where equals one-half of the objective's opening angle and is the refractive index of the immersion medium used between the objective and the cover slip protecting the specimen ( = 1 for air; = 1.51 for oil or glass). Numerical Aperture (NA)

12. The resolution of an optical microscope is defined as the smallest distance between two points on a specimen that can still be distinguished separate entities. Resolution is directly related to the useful magnification of the microscope and the perception limit of specimen detail, though it is a somewhat subjective microscopy because magnification, an image may appear out of focus but still be resolved to the maximum ability of the objective and assisting optical components as two value at in high Resolution