Understanding Microscopes: Light vs. Electron Microscopes

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Learn about the differences between light microscopes (LM) and electron microscopes (EM), including their magnification power, resolving power, and key parts. Explore the types of electron microscopes such as Transmission Electron Microscope (TEM) and Scanning Electron Microscope (SEM) for advanced imaging capabilities.


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  1. Microscope Light Microscope ElectronMicroscope

  2. Light Microscope (LM) The Magnification power of the LM is limited to 1000-2000 X. The Resolving power (resolution) of the LM is 0.2 m. The resolving power (resolution) is defined as the smallest distance between two particles at which they can be seen as separated objects. Note: 1 m = 1e-6 m

  3. PARTS 1. 2. 3. 4. Eyepiece Lens: Base: Stage: Revolving Nosepiece or Turret: Objective Lenses: Diaphragm or Iris: Coarse adjustment knob: Fine adjustment knob: 5. 6. 7. 8.

  4. Electron Microscope (EM) The Magnification power of the EM is limited to 2,000,000 X. The Resolving power (resolution) of the LM is 0.1 nm. The resolving power (resolution) is defined as the smallest distance between two particles at which they can be seen as separated objects. Note: 1nm = 1e-9 m

  5. Types: 1. Transmission Electron Microscope (TEM) 2. Scanning Electron Microscope (SEM)

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