Revolutionizing Protein Research with QDB ELISA Platform
Quanticision Diagnostics Inc. introduces the QDB ELISA platform, a cutting-edge solution for protein analysis. By addressing the limitations of traditional immunoassays, this innovative system offers enhanced sensitivity and specificity, making it ideal for quantifying complex samples such as FFPE tissues. With its unique design and versatile applications, QDB ELISA stands out as a game-changer in protein research.
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Quanticision Diagnostics, Inc DB 400 Park Offices Dr. RTP, NC 27709 www.quanticisiondiagnostics.com Dissection Of QDB Plate and Method
Popular Protein Research Platforms Immunoassay based Antibody-based) IHC Western blot Dot /slot blot ELISA RPPA QDB (Immunohistochemistry) (Enzyme-linked immunosorbent assay) (Reverse Phase Protein Array) (Quantitative Dot blot) ---newcomer Non-antibody based Spectrometry method (HPLC and mass spectrometry) -- specialized, mostly for novel protein identification.
All Immunoassays are Dot Blots in Essence Core step: immune reaction on a solid supporting matrix How do ELISA address this 1000 - fold binding deficiency? Selection of high protein-binding capacity matrix is key to sensitivity. Nitrocellulose Polystyrene Protein binding capacity Protein platforms 80-100 g/cm2 Western blot Dot/slot blot 100 to 200 ng /cm2 ELISA Protein Microarray slide QDB
ELISAs Efforts to Offset This Drawback Capture antibody* to enrich target protein binding Coating to mount the capture antibody to the polystyrene surface Optimize reagent to ensure: The matched antibody pair could work robustly The background caused by insufficient blocking efforts is reduced *Sandwich ELISA.
These Efforts are Double-Edged Upside: Matched antibody pair usage may increase specificity Downside: Finding a matched antibody pair is harder than finding a single antibody Difficult to optimize due to insufficient blocking in ELISA Assays for complex samples (e.g., cell/tissue) are extremely hard to develop Assays for FFPE samples are impossible
QDB Comes to the Rescue QDB plate is an ingeniously-designed 96-well plate Nitrocellulose membrane-based Each individual well has two openings on the side to facilitate the exchange of the solution during incubation and washing steps
Now, The Picture Is Complete QDB ELISA Only platform to quantify FFPE samples Best for blood samples Suitable for blood, urine, body fluid Suitable for urine, body fluid, fresh/frozen cells, tissues Coating and optimization required Needs matching antibody pair No required coating or optimization Expensive Inexpensive Hard to develop (takes months) Easy to develop (takes days)
QDB: Easy to Scale Up vs. RPPA QDB Microplate reader with chemiluminescence capacity in house Pros: Flexible with experimental designs 1 employee can process 3800 samples in a single run Digital results ---absolute, most accurate Inexpensive Sample usage is small ~ 0.5 g/sample RPPA Limited number of facilities nationwide Pros: Sample usage is tiny ~2ng/spot. Hundreds of samples each time Cons: Requires customary designed slides Expensive Results are image-based Not flexible with new samples Long waiting time for service(months)
QDB: Easy to Scale Down vs. Other Platforms QDB Quicker Simpler Digital results Small amount of sample High throughput Needs highly specific antibody Protein standard serves as a positive control Western, IHC Image-converted results. Low throughput Western: Tolerates less-specific antibodies Requires large amount of sample IHC: Provides morphology information. Needs highly specific antibody
Case Study 1: Use of QDB to Study Cell Proteins Determine the specificity of Antibody Quantity of p65 protein in 76 clone cell 0.5 mg/sample From 293 cells growing on 96 well plates we can get >12 g protein/well. Using only 0.5 g/assay we can perform 8 assays in triplicates. Antibody specificity is different in different cells
Case Study 2: Use of QDB to Study Animal Tissue Proteins Antibody specificity is different in different tissues 15 samples repeated 10 times Inter plate CV% : 4.37%~11.03%. Intra plate CV% :5.19%~9.03%. QDB needs < 1 g total protein
Case Study 3: Use of QDB to Analyze the Expression Level of Multiple Antibodies in Mouse Liver Tissue
Case Study 4: Use of QDB to Analyze Frozen Breast Cancer Tissue Recombinant protein Tissue lysate The result is highly concordant with clinical IHC results
Case Study 5: The Only High Throughput Immunity Analysis Method That Can Be Used On FFPE Sample Measuring the same sample group with two antibodies Tissue lysate The result is highly concordant with clinical IHC results
Review From a Satisfied Customer of 3 Years your product is quite unique because we have not found anything similar in the market that could quickly quantify protein amounts in a plate format, which is ideal for screening purposes ----Ruben Artero Full Professor, University of Valencia
An unexploited market No practical methods to measure majority of cell/tissue protein quantitatively. Developing ELISAs is time consuming and difficult Lacking access & high price tag to RPPA facilities. Research ElISA market: US: 128.7 Million 1398 Protein, 13% Protein in Blood, best by ELISA The sale market has no competitions due to patent protection . 9671 protein, 87% Prtoein in other tissues Best by Tissue Protein distribution ---The Human Protein Atlas * Protected by Patent US9933418 & Patent US10295544
Product and Service QDB plate QDB-based high throughput protein detection/quantitation service for industry or research institute Seeking motivated sales personnel or distributors
