Overview of Gene Transfer Methods in Molecular Biology
Explore various physical and chemical methods used for gene transfer, including electroporation, particle bombardment, microinjection, ultrasound, and bacterial transformation. Learn how DNA enters the nucleus and the role of retroviruses in gene transfer efficiency. Understand the process of RNA co
0 views • 17 slides
Gene Therapy and Genome Editing Technology
This article provides insights into the status, regulatory considerations, and delivery methods of gene therapy and genome editing technologies. It discusses approved gene therapies in the US, human genome editing in clinical applications, and the regulatory authority of the FDA over gene therapy. T
3 views • 27 slides
Understanding Viral Vectors in Gene Delivery: Safety and Considerations
Exploring the mechanisms of gene delivery via viral vectors, this content delves into the basics of viral structure, infection, and replication. It discusses common viral vector systems like Adenoviral and Lentiviral vectors, highlighting safety considerations and the production processes involved.
1 views • 19 slides
Understanding Gene Editing with CRISPR/Cas9 Technology
Explore the fascinating world of CRISPR/Cas9 gene editing technology through sessions led by experts like Roy Campbell and Howard Robinson. Delve into the working of gene editing, the CRISPR system, and the role of Cas9 enzyme. Learn about CRISPR's origin in prokaryotic organisms and its potential f
0 views • 33 slides
Understanding Gene Expression Regulation in Prokaryotes and Eukaryotes
Gene expression is a crucial process that leads to the production of functional gene products like RNA and proteins. This article explores the regulation of gene expression in both prokaryotes and eukaryotes, covering the importance of regulating gene expression, types of regulation elements, specif
5 views • 22 slides
Bioinformatics
Bioinformatics involves analyzing biological sequences through sequence alignment to uncover functional, structural, and evolutionary insights. This process helps in tasks like annotation of sequences, modeling protein structures, and analyzing gene expression experiments. Basic steps include compar
0 views • 6 slides
Understanding Artefacts and Biases in Gene Set Analysis
Gene set enrichment tests help identify functional gene sets enriched in hit lists compared to background sets. Various biases (technical, biological, statistical) can lead to incorrect conclusions in data analysis, emphasizing the importance of recognizing and addressing them. Technical biases like
0 views • 29 slides
Exploring Genetics and Genomics in Integrative Biology
Delve into the world of genetics and genomics through the lens of integrative biology, investigating the differences between cell types, the rationale for gene expression profiling, and the analysis of differential gene expression in various diseases. Uncover the significance of gene ontology, co-ex
0 views • 22 slides
Understanding Evolutionary Mechanisms: Natural Selection, Genetic Drift, and Gene Flow
Exploring the forces behind evolutionary change, this content delves into natural selection, genetic drift, and gene flow. Examples such as the bottleneck effect, founder effect, and Amish population polydactylism are presented to illustrate how these mechanisms shape genetic diversity. The impact o
0 views • 14 slides
Gene Prediction: Similarity-Based Approaches in Bioinformatics
Gene prediction in bioinformatics involves predicting gene locations in a genome using different approaches like statistical methods and similarity-based approaches. The similarity-based approach uses known genes as a template to predict unknown genes in newly sequenced DNA fragments. This method in
1 views • 40 slides
Understanding Epigenetics: DNA Methylation and Histone Modification
Epigenetics involves modifications that impact gene expression without altering DNA sequences, playing a crucial role in the transition from genotype to phenotype. This includes DNA methylation, histone modification, and microRNAs. DNA methylation, controlled by DNMT enzymes, can lead to either gene
5 views • 12 slides
Human Insulin Gene Expression and Production
The process of cloning and expressing the human insulin gene has revolutionized the production of insulin for treating diabetes. By using genetically engineered bacteria, the human insulin gene is inserted, expressed, and purified to create insulin for therapeutic use. This innovation has overcome c
0 views • 20 slides
Gene Expression Profiling in Statistical Genetics Summer Institute 2020
This content provides information on the Summer Institutes of Statistical Genetics module, focusing on gene expression profiling. It includes details on the schedule, experimental design, RNA sequencing workflow, modes of bulk RNA sequencing, and RNASeq software. The content discusses crucial steps
0 views • 11 slides
Understanding Gene Mutations in Molecular Biology
Gene mutations play a significant role in molecular biology, leading to alterations in DNA sequences that can impact offspring. These mutations can arise spontaneously or be induced by various factors, such as mutagens. Understanding gene mutations is crucial for comprehending the genetic basis of c
2 views • 27 slides
Understanding the Cre-Lox System for Genome Manipulation in Mice
The Cre-Lox system, a powerful tool in genetic engineering, employs Cre recombinase to recombine specific DNA sequences (LoxP sites), allowing for precise genetic modifications such as deletions, inversions, and translocations. This system enables conditional gene expression, turning on or off trans
4 views • 9 slides
Co-Developing Risk Assessments in Gene Drive
Gene drive technology is a cutting-edge approach aimed at combating malaria by spreading genetically modified genes in mosquito populations. This technology has the potential to protect endangered species and conserve nature. However, successful implementation requires complex collaborations, risk a
2 views • 16 slides
Understanding Epigenetics: DNA Methylation and Histone Modification
Epigenetics refers to changes in gene expression without altering the DNA sequence. This involves processes like DNA methylation, histone modification, and microRNAs. DNA methylation is controlled by DNA methyltransferase enzymes and plays crucial roles in gene activation and silencing. Histone modi
0 views • 13 slides
Understanding Gene Duplication, Mutation, and Read Mapping in Molecular Evolution
This presentation delves into the intricate concepts of gene duplication, DNA mutation, and read mapping in the context of molecular evolution. It explains the various types of mutations, the significance of gene duplication in generating new genetic material, and the process of read mapping to alig
0 views • 19 slides
Understanding Gene Therapy: Types, Techniques, and Applications
Gene therapy involves introducing DNA into a patient to treat genetic diseases by correcting disease-causing mutations. There are two types of gene therapy: somatic gene therapy and germline gene therapy, each with different implications. Techniques such as gene augmentation therapy and gene inhibit
1 views • 15 slides
Understanding Gene Regulation and Control of Gene Expression
This comprehensive content delves into the intricate mechanisms of gene regulation and control of gene expression. It covers topics such as transcriptional regulation, bacterial genes classification, and the role of regulatory proteins. Explore how genes are regulated at transcription, translation,
0 views • 17 slides
Understanding Gene Expression in Biological Sciences Course
Explore the dynamic world of gene expression with the Chunky and Chips Gene Expression Group at the University of Notre Dame. Dive into topics like transcription, translation, and cellular events influencing gene expression. Delve into real-world scenarios and gain insights into the cellular level b
0 views • 20 slides
Exploring the Effects of Gene Doping with PGC1a via Ormosil on Fitness in Mice
Investigating the potential of gene doping using PGC1a via Ormosil to enhance fitness outcomes in mice compared to exercise. Ormosil, a non-viral vector, is utilized for gene delivery, offering advantages in safety. Mice are divided into groups to receive treatment, exercise on treadmills, or remain
3 views • 8 slides
Comprehensive Assessment of a Pathogenic CNV in a 2-Year-Old Male with Developmental Delay and Seizures
In this case study, a 2-year-old male presenting with developmental delay and seizures was evaluated for a CNV at the 12p13.1p12.3 region. The CNV was found to overlap with the GRIN2B gene, a known haploinsufficient gene associated with developmental disorders. Despite potential additional scoring c
0 views • 17 slides
Exploring Biomedical Gene Dosage Compensation in Trisomy 21
This instructional material focuses on teaching gene dosage compensation in the context of addressing the inactivation of one copy of Chromosome 21 in trisomy 21. The activity is designed for courses in Molecular Gene Regulation and Genetics, aiming to enhance students' understanding through hands-o
0 views • 19 slides
Exploring Evolutionary Mechanisms: Genetic Drift, Gene Flow, Mutation, and Recombination
Delve into the effects of evolutionary mechanisms such as genetic drift, gene flow, mutation, and recombination in populations. Understand how changes in allele frequencies occur due to various factors like natural disasters and the bottleneck effect, impacting the gene pool and future generations.
1 views • 32 slides
Understanding Epistasis: Genetic Interactions and Their Implications
Epistasis is a phenomenon where the phenotypic expression of one gene is influenced by interactions with another gene. This concept, first introduced in 1909, plays a crucial role in genetics, affecting various traits and evolutionary processes. The difference between dominance and epistasis lies in
0 views • 41 slides
Understanding Transcription: The Initial Steps in Gene Expression
Gene expression involves two crucial phases - transcription and translation. Transcription, the first step, is the process where RNA is synthesized from DNA with the help of RNA polymerase. It begins with initiation, where the enzyme recognizes the promoter region and forms a complex with the DNA, m
0 views • 33 slides
Understanding Transposon Insertion Orientation and Its Impact on Gene Expression
This detailed guide explores the orientation of transposon insertions relative to their inherent asymmetry, like the P-element’s 5’ and 3’ ends, and how it influences constructs for driving ectopic gene expression. Using FlyBase resources, it explains the significance of orientation in constru
0 views • 5 slides
Evaluation of Maternally Inherited 3q26.32 Duplication in a 6-Year-Old Male with Autism
Maternal inheritance of a 3q26.32 duplication in a 6-year-old male with autism was assessed using the GAIN scoring metric. The duplication involves the TBL1XR1 gene and was found to fully overlap with a known haploinsufficient gene. The genomic content evaluation indicates the presence of one protei
0 views • 14 slides
Gene Wiki Jamboree FaceBase Spring Meeting Summary
The Gene Wiki Jamboree FaceBase Spring Meeting in 2010 aimed to create collaboratively-written and community-reviewed review articles for every gene in the human genome and every concept relevant to FaceBase. It highlighted the value of such articles, providing quick summaries and compilations of re
0 views • 13 slides
Raw Data of Agarose Gel for Human Gene Expression Analysis
This supplementary data includes raw data from agarose gel electrophoresis showing gene expression levels for human SOX17, FOXA2, AFP, ALB, and ACTB in various cellular samples. The gel images depict the expected sizes of amplicons for each gene in different conditions and time points of cell cultur
0 views • 5 slides
Understanding Splice Sites and Splicing Elements in the Inhibitory Dopamine Receptor Gene (D2)
In this project, the focus is on identifying splice sites relevant to the inhibitory dopamine receptor gene (D2). The problem involves finding splicing elements within a specific range of known splice sites in the gene. The data provided includes the D2 gene entry in FASTA format, a database of spli
0 views • 13 slides
Understanding Reverse Genetic Approaches in Gene Manipulation
Reverse genetic approaches involve mutating a target gene to observe its phenotypic effects, providing insights into the gene's normal function. Techniques like gene knockout, knockdown, and homologous recombination are used to manipulate genes and study genetic pathways, leading to discoveries in v
0 views • 6 slides
Basics of Hypothesis Testing in Gene Expression Profiling
The lecture covers the essential aspects of hypothesis testing in gene expression profiling, emphasizing experimental design, confounding factors, normalization of samples, linear modeling, gene-level contrasts, t-tests, ANOVA, and significance assessment techniques. Practical insights are shared on
0 views • 9 slides
Basics of Genetics Engineering: Introduction to DNA and Gene Expression
Exploring the fundamentals of genetics engineering, this lecture covers topics such as DNA structure, gene expression, gene function, genome, mutation, recombination, and genetic modification. Understand the significance of DNA, genes, and the processes involved in gene expression. Dive into the wor
0 views • 20 slides
Understanding Expression Vectors for Efficient Gene Expression
Expression vectors are designed to facilitate gene expression by carrying essential elements such as promoters, terminators, and fusion tags. They allow precise control over gene regulation for transcription and translation in host cells, enabling efficient protein production. Different promoters li
0 views • 15 slides
Cloning the Cstps-1 Gene from Citrus sinensis for Valencene Production
In this project, the Cstps-1 gene from Citrus sinensis is being cloned to produce the enzyme Citrus Valencene Synthase (CVS) responsible for generating the fragrant valencene compound. The process involves DNA isolation from a Valencia orange, gene amplification, vector transformation, and testing f
0 views • 8 slides
Understanding the Role of Multiple Enhancers in Gene Regulation
Multiple enhancers play a crucial role in regulating gene expression in a cell, with approximately 3-5 enhancers associated per human gene. These enhancers are essential for tissue-specific expressions and are active within the same cell line. The use of multiple enhancers can impact gene expression
0 views • 30 slides
Analysis of Proteasome Gene SNPs as Molecular Markers for Multiple Sclerosis in Latvian Population
This study by I. Trapina aims to investigate the prevalence and functionality of SNPs in proteasome genes to assess their potential as molecular markers for multiple sclerosis in the Latvian population. Multiple sclerosis is an autoimmune disease affecting the central nervous system, and the role of
0 views • 13 slides
Advances in Gene Therapy Programs and Best Practices Workshop Insights
Workshop outcomes and discussions from the MDA-NINDS Workshop on Best Practices for Gene Therapy Programs, highlighting key challenges, possible solutions, and the impact on translational funding programs. The workshop format included orienting talks and panels focusing on scientific premise, regula
0 views • 17 slides