Overview of Gene Transfer Methods in Molecular Biology
Explore various physical and chemical methods used for gene transfer, including electroporation, particle bombardment, microinjection, ultrasound, and bacterial transformation. Learn how DNA enters the nucleus and the role of retroviruses in gene transfer efficiency. Understand the process of RNA containing gene sequences of interest transduction. Visual aids and explanations enhance comprehension of complex molecular processes.
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Presentation Transcript
Physical gene transfer methods Electroporation Optimized
PMMA DEAE (Polymer beads) DNA Endocytosis occurs
Retrovirus mediated gene transfer Why retrovirus??? They have better recognition efficiency by different host cells
RNA containing gene sequence of interest Transduction cDNA cDNA
