Overview of Gene Transfer Methods in Molecular Biology

Explore various physical and chemical methods used for gene transfer, including electroporation, particle bombardment, microinjection, ultrasound, and bacterial transformation. Learn how DNA enters the nucleus and the role of retroviruses in gene transfer efficiency. Understand the process of RNA containing gene sequences of interest transduction. Visual aids and explanations enhance comprehension of complex molecular processes.

• Gene Transfer
• Molecular Biology
• Electroporation
• Retroviruses
• DNA Transduction

willem Follow

Uploaded on Mar 19, 2024 | 0 Views

Download Presentation

Please find below an Image/Link to download the presentation.

The content on the website is provided AS IS for your information and personal use only. It may not be sold, licensed, or shared on other websites without obtaining consent from the author. Download presentation by click this link. If you encounter any issues during the download, it is possible that the publisher has removed the file from their server.

Presentation Transcript

1. Methods of gene transfer

2. Physical gene transfer methods Electroporation Optimized

3. Particle bombardment

4. Microinjection

5. Ultrasound method

6. Chemical gene transfer methods

7. PMMA DEAE (Polymer beads) DNA Endocytosis occurs

11. BACTERIAL TRANFORMATION

14. How DNA enters the nucleus from cytoplasm?

15. Retroviruses

16. Retrovirus mediated gene transfer Why retrovirus??? They have better recognition efficiency by different host cells

17. RNA containing gene sequence of interest Transduction cDNA cDNA