Raw Data of Agarose Gel for Human Gene Expression Analysis

This supplementary data includes raw data from agarose gel electrophoresis showing gene expression levels for human SOX17, FOXA2, AFP, ALB, and ACTB in various cellular samples. The gel images depict the expected sizes of amplicons for each gene in different conditions and time points of cell culture. Explore the agarose gel results to analyze the gene expression patterns in human iPSCs across different culture stages.

• gene expression analysis
• agarose gel electrophoresis
• human cells
• iPSC culture
• amplicon size

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Uploaded on Sep 27, 2024 | 0 Views

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1. Supplementary data: Raw data of agarose gel with labels Human SOX17 M 1 2 3 4 5 6 NTC bp 3000 1500 1000 800 500 400 300 200 100 M: 100-bp DNA ladder 1: Human iPSCs 019 2: 5-Day 2D culture of human iPSCs 019 3: 10-Day 2D culture of human iPSCs 019 4: 15-Day 2D culture of human iPSCs 019 5: 25-Day 2D culture of human iPSCs 019 6: 17-Day 3D culture of human iPSCs 019 NTC: no template control Arrow: expected size of amplicons

2. Supplementary data: Raw data of agarose gel with labels Human FOXA2 M 1 2 3 4 5 6 NTC bp 3000 1500 1000 800 500 400 300 200 100 M: 100-bp DNA ladder 1: Human iPSCs 019 2: 5-Day 2D culture of human iPSCs 019 3: 10-Day 2D culture of human iPSCs 019 4: 15-Day 2D culture of human iPSCs 019 5: 25-Day 2D culture of human iPSCs 019 6: 17-Day 3D culture of human iPSCs 019 NTC: no template control Arrow: expected size of amplicons

3. Supplementary data: Raw data of agarose gel with labels Human AFP M 1 2 3 4 5 6 NTC bp 3000 1500 1000 700 500 400 300 200 100 M: 100-bp DNA ladder 1: Human iPSCs 019 2: 5-Day 2D culture of human iPSCs 019 3: 10-Day 2D culture of human iPSCs 019 4: 15-Day 2D culture of human iPSCs 019 5: 25-Day 2D culture of human iPSCs 019 6: 17-Day 3D culture of human iPSCs 019 NTC: no template control Arrow: expected size of amplicons

4. Supplementary data: Raw data of agarose gel with labels Human ALB M 1 2 3 4 5 6 NTC bp 3000 1500 1000 700 500 400 300 200 100 M: 100-bp DNA ladder 1: Human iPSCs 019 2: 5-Day 2D culture of human iPSCs 019 3: 10-Day 2D culture of human iPSCs 019 4: 15-Day 2D culture of human iPSCs 019 5: 25-Day 2D culture of human iPSCs 019 6: 17-Day 3D culture of human iPSCs 019 NTC: no template control Arrow: expected size of amplicons

5. Supplementary data: Raw data of agarose gel with labels Human ACTB M 1 2 3 4 5 6 NTC bp 3000 1500 1000 600 500 400 300 200 100 M: 100-bp DNA ladder 1: Human iPSCs 019 2: 5-Day 2D culture of human iPSCs 019 3: 10-Day 2D culture of human iPSCs 019 4: 15-Day 2D culture of human iPSCs 019 5: 25-Day 2D culture of human iPSCs 019 6: 17-Day 3D culture of human iPSCs 019 NTC: no template control Arrow: expected size of amplicons