Gel electrophoresis - PowerPoint PPT Presentation


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Gel Electrophoresis DNA Extension Activity in Modern Biology Lab

This lab activity uses agarose gel electrophoresis to determine the length of an unknown DNA fragment by comparing it to known DNA fragment lengths. The process involves gel preparation, loading samples, electrophoresis, staining, de-staining, and measurement of DNA bands. The activity was produced

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Pulsed-Field Gel Electrophoresis: Separating Large DNA Molecules

Pulsed-Field Gel Electrophoresis (PFGE) is a technique developed to effectively separate large DNA molecules through the application of an electric field that periodically changes direction. This method, introduced by David C. Schwartz and Charles C. Cantor in 1984, revolutionized the resolution of

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Understanding Blotting Techniques in Animal Biotechnology

Blotting techniques such as Southern blot, Northern blot, and Western blot are used to transfer DNA, RNA, and proteins onto a carrier for separation following gel electrophoresis. Developed by E.M. Southern, the Southern blot specifically detects DNA fragments in a sample through hybridization. This

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Understanding DNA Sequencing: Principles, Applications, and Techniques

DNA sequencing plays a vital role in various fields such as research, diagnostics, biotechnology, forensics, and biological systematics. By determining the order of nucleotide bases in a DNA molecule, it helps in understanding genetic sequences, identifying mutations, and completing projects like th

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PCR and Blot Techniques in Molecular Biology

Polymerase Chain Reaction (PCR) is a crucial technique for amplifying specific DNA segments rapidly and accurately. Developed in 1983, PCR is widely used in various fields such as molecular biology, forensics, evolution, and medical diagnostics. On the other hand, the Blot technique transfers DNA, R

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Understanding Electrophoresis: Principles, Techniques, and Applications

Electrophoresis is a technique dating back to principles of electrochemistry, involving the movement of charged particles in an electric field for separation. Factors affecting electrophoretic mobility include charge, particle size, shape, and applied electrical field. Techniques such as paper, gel,

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Analysis of Experiment Results: Group 20 Presentation

This presentation showcases the experiment results of Group 20, including findings on food samples, gel electrophoresis data, and implications of potential contamination in plant material and GMO presence. The team consists of Canberk from Turkey, Mehmet Tekinalp, and Elin from Denmark's Thisted Gym

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Understanding Genetic Engineering and Selective Breeding

Genetic engineering involves changing the DNA in living organisms to create new traits, resulting in genetically modified organisms (GMOs). Techniques include artificial selection, cloning, gene splicing, and gel electrophoresis. Selective breeding is a form of artificial selection where animals wit

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Laboratory Equipment Maintenance Guidelines for Daily, Weekly, and Monthly Tasks

Regular maintenance tasks for various laboratory equipment including analyzers, flow cytometer, automated gel electrophoresis, and micro hematocrit. The guidelines cover daily, weekly, and monthly checks, cleanings, replacements, and verifications to ensure proper functioning and accurate results. T

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Explore the power of GEL scripting in Clarity Educational Community with sessions on script exercises, prerequisites, automation benefits, and data sources. Understand what GEL is, its execution language, and how it enables custom logic in CA PPM. Dive into GEL basics, automation advantages, and scr

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Understanding Agarose Gel Electrophoresis in Biochemistry and Molecular Biology

Agarose gel electrophoresis is a technique used to separate and analyze DNA or RNA molecules based on size in biochemistry and molecular biology. It involves applying an electric field to move molecules through an agarose matrix towards the positive electrode, with larger molecules moving slower tha

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Understanding Western Blotting for Protein Detection in Biochemistry

Western blotting, also known as protein immunoblot, is a crucial technique for identifying specific proteins based on their ability to bind to antibodies. It involves electrophoresis of protein samples on an SDS-PAGE gel, transferring them to a nitrocellulose membrane, and detecting them using speci

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Gel Electrophoresis for Assessing Nucleic Acid Quality

Gel electrophoresis is a crucial technique for assessing the quality and yield of nucleic acids such as DNA and RNA. It separates DNA fragments based on size, allowing researchers to determine the integrity of the nucleic acids. By running a small amount of sample on a gel and using DNA-binding dyes

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Understanding Gel Permeation Chromatography (GPC) Technique

Gel Permeation Chromatography is a technique used for separating components based on molecular weight or size. It utilizes columns packed with a porous material to separate molecules by size, allowing smaller molecules to penetrate the gel matrix while larger ones pass through unhindered. Learn abou

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Understanding Quality Control and Electrophoresis Techniques

Exploring the process of analyzing product quality through techniques like polyacrylamide gel electrophoresis, evaluating purity and potency using various testing methods such as cell biology and chemistry. Understanding macromolecular charges, movement of molecules, and the use of agarose and polya

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Raw Data of Agarose Gel for Human Gene Expression Analysis

This supplementary data includes raw data from agarose gel electrophoresis showing gene expression levels for human SOX17, FOXA2, AFP, ALB, and ACTB in various cellular samples. The gel images depict the expected sizes of amplicons for each gene in different conditions and time points of cell cultur

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Gel Filtration Chromatography: Separation and Molecular Weight Determination

Gel filtration chromatography is a method used for separating proteins based on their molecular weights. This experiment aims to teach students the principles of gel filtration chromatography and provide hands-on experience in the lab. By utilizing a stationary phase of porous beads and a mobile pha

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Gel Electrophoresis in Genetic Engineering: Principles and Types

Gel electrophoresis is a crucial technique in genetic engineering used to separate macromolecules based on size and charge. Agarose and polyacrylamide gels are the two main types used, each with its advantages and disadvantages. Agarose gel electrophoresis is effective for separating large DNA molec

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Thermal Shock Testing of Gel-Bonded Magnesia Refractories

Study conducted by Paul Bosomworth at Leeds University in 1986 aimed to reduce mechanical damage after thermal shock in magnesia refractories used in sliding gates. The research focused on using gel-binders and achieving optimum grain packing to enhance thermal stress damage resistance. The study al

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Understanding Agarose Gel Electrophoresis: A Comprehensive Overview

Agarose gel electrophoresis is a vital technique used to separate DNA, RNA, or proteins based on their physical properties like size and charge. This seminar covers the definition, working process, and applications of agarose gel electrophoresis, shedding light on its importance in molecular biology

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Understanding Nucleic Acid Blotting: Southern Blotting Techniques

Nucleic acid blotting techniques, including Southern blotting, are crucial for transferring DNA, RNA, and proteins for analysis. Southern blotting, developed by Edwin Mellor Southern, is used to probe specific DNA sequences, aiding in applications such as gene isolation, DNA fingerprinting, and dise

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Understanding Gel Filtration Chromatography: Principles and Applications

Gel filtration chromatography is a molecular size-based separation technique used to separate macromolecules of different sizes. This method, also known as gel permeation or size exclusion chromatography, utilizes a porous stationary phase where small molecules penetrate all pores and are retained,

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Understanding Paper Electrophoresis in Biochemistry Research

Paper electrophoresis is a powerful technique used in biochemistry to separate charged particles like proteins based on their migration along a filter paper strip. This method, conducted by Dr. Nisha Sharma, an Associate Professor at C.S.J.M. University in Kanpur, involves applying samples to the pa

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Understanding the Chemical and Physical Properties of DNA

DNA's characteristics such as absorption of UV light, density measurement using cesium chloride, and size estimation through electrophoresis are key in molecular biology research. The hyperchromic effect, UV light absorption at 260nm, CsCl-density ultracentrifugation, and gel electrophoresis are cru

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Exploring Biotechnology: Concepts and Techniques

Biotechnology encompasses a range of techniques and processes aimed at manipulating biological systems for various applications. This includes methods like PCR for DNA amplification, DNA extraction, gel electrophoresis for DNA fragment analysis, and restriction enzyme processes for DNA manipulation.

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Understanding DNA Fragment Analysis Through Polyacrylamide Gel Electrophoresis

Polyacrylamide gel electrophoresis is a technique used to separate DNA fragments based on size. By casting a gel and running an electric current through it, terminated DNA fragments can be separated and visualized using isotopes. This process allows for the analysis of DNA fragments differing in siz

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Analysis of Bacterial and Fungal Communities in Forested and Shortgrass Steppe Soil

The study conducted in July 2010 at the Summer Soil Institute involved a Quantitative PCR analysis of bacterial and fungal communities in Forested and Shortgrass Steppe Soil. The research compared the microbial DNA extracted from different sites and depths in these ecosystems, using techniques such

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How to Choose the Right Hydrating Gel Moisturizer

Finding the perfect hydrating gel moisturizer can be challenging, especially with the range of options available today. As skincare needs vary by skin type, climate, and individual preference, selecting a hydrating gel tailored to your needs ensures

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