Cell culture techniques - PowerPoint PPT Presentation


Vi-CELL.BLU - Advanced Cell Counting Instrument

Vi-CELL.BLU is a cutting-edge cell counting instrument that offers faster analysis, increased resolution, and improved optical sensor technology for enhanced cell concentration and viability assessments. The device features a user-friendly interface, Trypan blue method for live cell detection, and a

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Understanding Virus-Cell Interactions: Mechanisms and Consequences

Viruses interact with host cells in various ways, encoding genes that manipulate cell functions for their benefit. These interactions can range from benign to lethal outcomes. Factors influencing these interactions include viral factors, cellular responses, and the presence of virulence factors. Dif

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Understanding Cell Reproduction and the Cell Cycle

Explore the process of cell reproduction, DNA structure, chromosomes, and the differences between prokaryotic and eukaryotic cell cycles. Learn about binary fission, mitosis, and cytokinesis in the context of cellular growth and division. Understand the significance of genes, DNA organization into c

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Understanding Cell Viability Assays in Laboratory Testing

Cell viability assays play a crucial role in determining the health and status of cells, measuring their ability to survive and proliferate. These assays involve various techniques such as dye exclusion, colorimetric, fluorometric, luminometric, and flow cytometric assays. By assessing factors like

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Understanding Cell Division: Functions and Stages

Explore the functions and stages of cell division through a comprehensive overview covering topics such as the importance of cell division in growth, repair, and reproduction, the stages of the cell cycle, and the comparison of different cell cycles. Engage with visual aids and concept maps to deepe

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Bacterial Cell Structure and Composition Overview

Bacterial cells exhibit variations in size, typically ranging from 0.75 to 1.5 micrometers. The cell envelope, comprising glycocalyx, cell wall, and cell membrane, plays crucial roles in protection and cell function. The cell membrane, a thin barrier rich in phospholipids and proteins, is integral t

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Understanding Plant Tissue Culture: Methods and Requirements

Plant tissue culture involves the in-vitro culture of plant explants under aseptic conditions, covering cell, organ, and suspension cultures. This process, pioneered by German botanist Gottlieb Haberlandt, relies on the totipotency of plant cells. Key requirements include laboratory organization, su

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An Overview of Cell Biology: From Cytology to Modern Studies

Cell biology, also known as cytology, explores cells from various perspectives including physiological, developmental, and evolutionary aspects. Modern Cell Biology delves into the mechanisms of metabolism, heredity, and evolution at the molecular level, involving proteins, RNAs, and DNA. The compar

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Understanding BD FACS Aria III for Efficient Cell Sorting

Learn about the BD FACS Aria III, a sterile cell sorter capable of analyzing up to 15 colors and sorting up to 4 populations simultaneously. Recommended sample concentrations, sorting procedures, and tube preparation guidelines are provided for efficient cell sorting. Discover important tips to ensu

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Overview of Cell Culture Methods and Importance in Research

Introduction to the principles of cell culture, including tissue culture, organ culture, and cell culture methods. Discusses the advantages and disadvantages of each technique and highlights the need for cell culture in research for studying cellular behavior and large-scale production of cell mater

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Overview of Bacterial Morphology and Cell Structure

This article discusses the morphology of both Gram-positive and Gram-negative bacteria, highlighting examples of different arrangements and shapes. It also explores the structure and functions of bacterial cell walls, emphasizing the role of teichoic acids in Gram-positive cell walls. Additionally,

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Cell Structure and Function: A Comprehensive Overview

Delve into the world of cells, exploring the distinct features of animal and plant cells, the differences between prokaryotic and eukaryotic cells, the significance of organelles, and the fascinating processes such as stem cell differentiation and cell adaptation. Discover how substances move across

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MTT Cell Proliferation Assay: Protocol and Applications

The MTT cell proliferation assay is a widely used technique to measure cell viability, proliferation, and cytotoxicity in research. This colorimetric assay involves the conversion of MTT reagent to formazan dye by live cells, resulting in a purple color change. This comprehensive guide provides step

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Understanding 10X Single Cell RNA-Seq Analysis

This content delves into the intricacies of analyzing 10X single-cell RNA-Seq data, covering topics such as how 10X RNA-Seq works, evaluating CellRanger QC reports, dimensionality reduction techniques like PCA, tSNE, and UMAP, using the Loupe cell browser, and frameworks for scRNA analysis in R. It

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Understanding Bacterial Cell Structure and Function

Bacteria display unique cellular structures and functions that differ from eukaryotic cells. They have a simple structure with a plasma membrane but lack complex internal membrane systems. The cytoplasm contains inclusion bodies, ribosomes, and genetic material in the nucleoid. Bacteria can be categ

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Regulation of the Cell Cycle: A Comprehensive Overview

Variation in cell cycle length in humans, controlled by internal and external mechanisms, with special proteins and checkpoint systems ensuring proper progression. External events trigger initiation and inhibition of cell division, while internal checkpoints maintain genetic integrity and chromosome

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Meticulous Research® Forecasts Cell Culture Market to Reach $48.63 Billion by 2029, Growing at a CAGR of 11.7%”

Cell Culture Market by Product [Consumables (Media, Reagents, Sera, Cell Lines), Equipment (Bioreactor, Centrifuge, Cell Counter)], Application (Bioproduction, Cancer Research, Stem Cell, Diagnostic), End User (Pharma, academic) - Global Forecast to

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Understanding the Cell Cycle and Mitosis Process

The cell cycle consists of two main periods: Interphase and Mitosis. During Interphase, the cell prepares for division by growing in size and copying chromosomes. Mitosis, the division of the nucleus, results in the formation of two daughter cells with identical chromosome copies. Centrioles and cen

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Understanding Cell Division: Processes and Types

Cell division is a vital process in living cells for growth and reproduction. This article explores the basics of cell division, including the cell cycle, types of cell division (such as mitosis and meiosis), and the initiation of cell division. It also covers key phases like interphase and provides

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Plant Tissue Culture Methods for Growth and Reproduction Study

Plant tissue culture methods such as root tip culture, shoot-tip culture, leaf culture, flower culture, and anther and pollen culture allow for the study of growth, reproduction, and genetic variations in plants. These techniques involve culturing various plant parts under sterile conditions to inve

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Global Cell Culture Market Set to Soar to $48.63 Billion by 2029, Driven by Strong Growth in Biopharma and Rising Demand for Monoclonal Antibodies

Cell Culture Market by Product [Consumables (Media, Reagents, Sera, Cell Lines), Equipment (Bioreactor, Centrifuge, Cell Counter)], Application (Bioproduction, Cancer Research, Stem Cell, Diagnostic), End User (Pharma, academic) - Global Forecast to

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Understanding Cell Injury and its Causes in Pathology

Rudolph Virchow's concept of disease starting at the cellular level is explored in this content, focusing on the impact of the external environment on cell equilibrium. The role of the plasma membrane as a barrier and the definitions of normal cell function, adaptation, reversible injury, irreversib

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Implementation of Cell Phone Policy to Improve English Grades at Richwoods High School

Implementation of a cell phone policy at Richwoods High School was driven by the need to enhance English grades and academic performance. The policy aims to reduce distractions and disruptions caused by non-academic cell phone use in classrooms. Results from previous semesters showed significant imp

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Explore the World of Developmental and Cell Biology

Developmental biology, stemming from embryology, investigates how organisms evolve from a zygote. It merges various fields like genetics, cell biology, and cancer biology. Cell biology focuses on cell properties and behaviors, using tools like genomics and microscopy. Research areas include cancer c

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Role of Cell Cycle in Nanoparticle Uptake and Dilution in Cell Population

The cell cycle plays a crucial role in the cellular uptake and dilution of nanoparticles within a cell population. This process involves different phases such as G1, S, G2, and M, each with specific functions related to cell growth, DNA synthesis, protein synthesis, and cell division. Understanding

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Fascinating Vocabulary of Cell Biology

Explore the intricate world of cell biology through fancy terminology for basic cell components, such as the cell membrane, cytoplasm, mitochondria, nucleus, and more. Discover the unique functions and structures of plant and animal cells as compared to different types of cells within each class. Vi

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Understanding Cell Organelles and Their Functions

Cell organelles play vital roles in the functioning of both animal and plant cells. From the cell membrane that controls the exchange of materials to the nucleus that governs cell activities, each organelle contributes uniquely. The cytoplasm provides a supportive environment for organelles, while t

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Understanding the Significance of the Cell Cycle in Growth and Development

This resource focuses on the cell cycle in the context of growth, development, and proliferation. It covers the significance of cell division, similarities in unicellular and multicellular organisms, the importance of CDK-mediated phosphorylation, and more. Students will learn to create schematic de

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Exploring Cell Structure and Function in Developmental Biology

Discover the intricate world of cell structure and function in the context of developmental biology. Delve into the inner workings of organelles, their functions, and how they contribute to cellular activities. Engage in interactive activities like building cell models and playing Cell Detective to

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An Overview of Cell Culture Techniques

Cell culture is a vital laboratory technique used in various fields of science to grow and maintain cells outside their natural environment. The process involves cell isolation, maintenance in a suitable environment, sub-culturing, and incubation in controlled conditions. Basic equipment like weighi

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Understanding Stem Cells and Cell Potency in Animal Cells

Stem cells play a crucial role in animal cells, offering the potential to differentiate into various cell types. Totipotent stem cells are the most versatile, capable of developing into any cell type in the embryo, including extra-embryonic cells. Pluripotent stem cells can give rise to all body cel

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Understanding Cell Cycle Control in Biology

Maintaining control of the cell cycle is crucial to producing healthy daughter cells and preventing mutations that can lead to degenerative diseases like Parkinson's or cancer. Cell cycle checkpoints at G1, G2, and Metaphase ensure the cell meets specific requirements before progressing to the next

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Understanding Plant Cell Walls: Structure and Function

Plant cell walls play a crucial role in providing structure and protection to plant cells. This article delves into the composition of primary and secondary cell walls, highlighting the presence of cellulose, pectins, and glycans. The secondary cell wall, rich in lignin, offers mechanical support, e

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Cell-to-Cell Communication in Introductory Biology Class for Non-Majors

Explore the fascinating world of cell-to-cell communication in an introductory biology class for non-majors led by Christine Andrews, Katie Morrison-Graham, Bianca Breland, Marilyn Thomas, Sarah Cabbage, and Kat Milligan-Myhre. Understand the vital role of cell signaling pathways, the impact on drug

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Understanding Cell Structure and Function

Explore the intricate world of cell biology through this comprehensive guide covering the definition of a cell, the characteristics of animal cells, the role of the nucleus, ribosomes, cell structure, organelles, cell membrane, and cell wall. Discover the fascinating details of eukaryotic cells and

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Understanding the Functions of Cell Organelles

Explore the vital functions of different cell organelles such as the cell membrane, nucleus, endoplasmic reticulum, lysosomes, Golgi apparatus, mitochondria, cytoplasm, ribosomes, vacuole, cell wall, chloroplasts, and chlorophyll in a cell. Learn how each organelle plays a unique role in maintaining

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Basics of Mammalian Cell Culture

Mammalian cell culture involves the removal of cells from an organism for growth in a controlled environment. Primary cell cultures can be sub-cultured, leading to the generation of cell lines. Sub-culturing involves transferring cells to fresh growth media for further growth. Different methods and

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Cultivating a Successful Culture in Neurology Department

Importance of culture in a neurology department highlighted by Dr. Robin L. Brey. Emphasis on how culture trumps strategy and the role of leaders in maintaining a positive culture. Examples and insights on how culture influences decision-making and behavior. Attributes of a successful culture and ho

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Understanding Animal Tissue Culture and Cell Line Production

Animal tissue culture involves growing tissues separate from the animal in a laboratory setting. To achieve exponential cell growth, cells are converted into immortal cell lines. The production of a cell line involves steps like breaking cell adhesion, incubation, and transferring cells to fresh med

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Cell Culture Market, Reaching $48.63 Billion by 2029

Cell Culture Market by Product [Consumables (Media, Reagents, Sera, Cell Lines), Equipment (Bioreactor, Centrifuge, Cell Counter)], Application (Bioproduction, Cancer Research, Stem Cell, Diagnostic), End User (Pharma, academic) - Global Forecast to

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