Understanding Wool Fat: Properties, Sources, and Uses

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Wool fat, also known as lanolin, is a pale yellow substance extracted from raw wool. It contains cholesterol, alcohols, and fatty acids and is insoluble in water. Prepared from sheep wool, wool fat is used in creams and ointments as an emollient base. Its purification involves processes like cracking, centrifuging, and bleaching. Testing for cholesterol involves a distinct color reaction. Proper storage is in closed containers away from light.


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  1. Wool fat Dr. Anju Singh Assistant Professor SPS, CSJMU, Kanpur

  2. description Raw wool contains considerable quantities of 'wool grease' or crude lanolin, the potassium salts of fatty acids and earthy matter. Wool fat is a pale yellow, tenacious substance with a faint but characteristic odour. It is insoluble in water and a high proportion of water may be incorporated with it by melting (m.p. 36-42 C) and stirring. Soluble in ether and chloroform.

  3. Source of Wool fat Wool fat (anhydrous lanolin) is a purified fat-like substance prepared from the wool of the sheep, Ovis aries (Bovidae).

  4. Chemical constituent The chief constituents of wool fat are cholesterol and iso-cholesterol, unsaturated monohydric alcohols of the formula C27H45OH, both free and combined with lanoceric, lanopalmitic, carnaubic and other fatty acids. Wool fat also contains aliphatic alcohols such as cetyl, ceryl and carnaubyl alcohols. Butylated hydroxytoluene, up to 200 p.p.m., may be added as an antioxidant. Wool alcohols are prepared by the saponification of crude lanolin and the separation of the alcohol fraction. The product consists of steroid and triterpene alcohols, including cholesterol (not less than 30%) and isocholesterol.

  5. Preparation Raw lanolin is separated by 'cracking' with sulphuric acid from the washings of the scouring process and purified to fit it for medicinal use. Purification may be done by centrifuging with water and by bleaching.

  6. Evaluation To test for cholesterol dissolve 0.5 g in 5 ml of chloroform, add 1 ml acetic anhydride and two drops of sulphuric acid; a deep-green colour is produced,

  7. Storage It is stored in closed containers away from light and in cool place. uses Wool fat is used as an emollient base for creams and ointments.

  8. THANK YOU

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