Wheat Germ DNA Extraction Protocol - Salk Institute Mobile Lab
Follow the detailed steps provided by the Salk Institute Mobile Lab to extract DNA from wheat germ. This protocol includes mixing wheat germ with water, adding dish soap, using a hot water bath, and isolating DNA with isopropanol. Learn the process and understand the science behind each step.
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DNA Extraction from wheat germ Protocol Salk Institute Mobile Lab Salk Institute Mobile Lab
Step # 1 Obtain a numbered 50 ml conical vial with 10 ml of water. Write your number here:________ Salk Institute Mobile Lab
Step # 2 To the tube with 10 ml water add 1g (1 pinch) of raw wheat germ. The wheat germ should settle to fill 1/2 the cone Salk Institute Mobile Lab
Step # 3 Cap the tube and vigorously shake to mix for about 30 seconds. What do you think the shaking is doing to the wheat cells? Salk Institute Mobile Lab
After shaking - let the cell walls settle to the bottom What s in the cloudy solution? Where is the DNA at this point? Salk Institute Mobile Lab
Step # 4 Using a transfer pipet add 3 ml of 25% dish soap to the tube with water and wheat germ. Salk Institute Mobile Lab
Steps # 5-6-7 Cap the tube and slowly invert several times to mix (DO NOT SHAKE). Loosen the cap slightly before placing tube in the hot water bath. Heat at 65 C for 5 minutes. Salk Institute Mobile Lab
What do you think the soap will do? How will the hot water bath help? Bonus question: Why does washing your hands with soap and hot water kill bacteria on your skin? Salk Institute Mobile Lab
Steps # 8-9 While the tubes are heating carefully fill a 1.7 ml eppendorf tube half way ( ) full with isopropanol. Carefully place the open filled epi into the rack Prep for the next step by filling a transfer pipet with 3ml of isopropanol (leave in stock isopropanol tube) Salk Institute Mobile Lab
Step # 10 Bring the 50 ml conical tube back to your table and as soon as possible add the 3ml of isopropanol to the tube. Salk Institute Mobile Lab
Step # 11 Watch the DNA rise out of the green soap layer and into the isopropanol layer. What color is DNA?? What color do you think YOUR DNA is? Why? Salk Institute Mobile Lab
Step # 12 After waiting a few moments to allow the DNA to precipitate remove the DNA using an inoculation loop and place in your eppendorf tube. Try to avoid any wheat germ debris that is present. Salk Institute Mobile Lab
Step # 12 continued Spinning the loop will help get the DNA off Make sure to close the epi tube securely (you should hear it click ) Label your tube with your initials Salk Institute Mobile Lab
Congratulations! You have just done something that scientists do everyday in the lab! Your DNA will last you for years it s very stable when stored in isopropanol SOME RULES: Your DNA tube should be put away during school - other teachers will confiscate it if you disturb their class! You must show your parents at home your DNA and tell them what you learned! Salk Institute Mobile Lab
Review: We extracted DNA from wheat germ And discovered that the color of DNA is WHITE! No matter what organism it comes from, DNA in a tube looks the same! Salk Institute Mobile Lab
We ALL have white DNA!! Salk Institute Mobile Lab
