Pure Culture Preparation by Streak Plate Method
Aim:
To prepare pure culture by streak
plate method.
Theory
:
Generally bacteria exist in mixed population. It
is very rare to get a single and pure form. For
studying the cultural, morphological and
physiological characters of an individual
species it is essential to separate them from
the others to get in the form called pure
culture . There are many important methods
for isolating pure culture from mixed culture
like streak plate method, pour plate methods
etc.
Requirements :
•
Tripod and wire gauze
•
Bunsen burner
•
Beaker of water
•
Inoculating loop
•
Nutrient agar
•
Sterile Petri dish
•
Mixed culture
Procedure:
1.
Liquefy a tube of nutrient agar and pour into the
petridish, rotate the plate gently for uniform
distribution of the medium.
2.
Streak the plate following quadrant or radiant or
T-streak or continuous streak .
3.
Keep the streaked plates in inverted position at
37°C for over night (approximately 16 hrs).
4.
Place the petri dish upside down to solve the
problem of water condensation because if it drops
down on the colonies , the organisms of one
colony can spread on the other colony.
Results:
•
The isolated colonies of desired
microbes (at the site of last streak)
on the plate will be observed.
Bacteria often exist in mixed populations, making it essential to isolate them for studying their characteristics. The streak plate method, involving streaking nutrient agar and incubating, helps in obtaining pure cultures of individual species. This method facilitates the observation of isolated colonies of desired microbes for further analysis.
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Presentation Transcript
Aim: To prepare pure culture by streak plate method.
Theory: Generally bacteria exist in mixed population. It is very rare to get a single and pure form. For studying the cultural, morphological and physiological characters species it is essential to separate them from the others to get in the form called pure culture . There are many important methods for isolating pure culture from mixed culture like streak plate method, pour plate methods etc. of an individual
Requirements : Tripod and wire gauze Bunsen burner Beaker of water Inoculating loop Nutrient agar Sterile Petri dish Mixed culture
Procedure: 1. Liquefy a tube of nutrient agar and pour into the petridish, rotate the plate gently for uniform distribution of the medium. 2. Streak the plate following quadrant or radiant or T-streak or continuous streak . 3. Keep the streaked plates in inverted position at 37 C for over night (approximately 16 hrs). 4. Place the petri dish upside down to solve the problem of water condensation because if it drops down on the colonies , the organisms of one colony can spread on the other colony.
Results: The isolated colonies of desired microbes (at the site of last streak) on the plate will be observed.
