Genetic Engineering Techniques and Enzyme Palindromes

Genetic Engineering Techniques and Enzyme Palindromes
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Explore the GGA method with pClone Red, EcoRI palindrome type II variations, and BsaI enzyme properties in genetic manipulation. Detailed images and descriptions provide insights into DNA cutting techniques and promoter sequences.

  • Genetic engineering
  • Enzyme palindromes
  • DNA manipulation
  • EcoRI
  • BsaI

Uploaded on Oct 03, 2024 | 0 Views


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  1. GGA Method with pClone Red by A. Malcolm Campbell and Todd Eckdahl

  2. Eco RI G CTTAA G AATTC palindrome type II

  3. Eco RI G CTTAA G AATTC palindrome type II

  4. Eco RI G CTTAA G AATTC type II

  5. Eco RI G CTTAA AATTC G type II

  6. Bsa I GAGACC CTCTGG not a palindrome type IIs

  7. Bsa I 1234nGAGACC nCTCTGG ---- type IIs

  8. Bsa I 1234nGAGACC nCTCTGG ---- type IIs

  9. Bsa I GGTCTCn ---- CCAGAGn1234 type IIs

  10. Bsa I GGTCTCn CCAGAGn1234 ---- type IIs

  11. Bsa I 1234nGAGACC ----nCTCTGG GGTCTCn---- CCAGAGn1234 cuts left cuts right

  12. Part J119137 BsaI cuts left BsaI cuts right

  13. CGACtGAGACC GGTCTCa aCTCTGG CCAGAGtCGCC Bsa I GCGG GCTG Bsa I

  14. CGACtGAGACC GGTCTCa aCTCTGG CCAGAGtCGCC GCGG GCTG

  15. CGACtGAGACC GGTCTCa aCTCTGG CCAGAGtCGCC GCTG GCGG

  16. CGACtGAGACC GGTCTCa aCTCTGG CCAGAGtCGCC GCGG GCTG CGAC(promoter) (promoter)CGCC

  17. CGACtGAGACC GGTCTCa aCTCTGG CCAGAGtCGCC CGAC(promoter) GCGG GCTG (promoter)CGCC

  18. GGA Ligation Method RBS GFP RBS RFP BsaI + Ligase origin antibiotic resistance plasmid backbone

  19. GGA Ligation Method RBS GFP RBS RFP BsaI + Ligase origin antibiotic resistance plasmid backbone

  20. GGA Ligation Method

  21. GGA Ligation Method

  22. GGA Ligation Method X

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