Enzyme Activity and Optimal Conditions
Enzyme Station Activity
INTERPRETING DATA & GRAPHS
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1. The enzyme would most likely
affect
reactions
involving
A.
molecule A, only
B.
molecule C, only
C.
molecules B and D
D.
molecules A and C
2. Which graph, A or B, shows the
amount of substrate
going to zero faster?
3. If both graphs A and B show
the rate of an enzyme
,
which enzyme is more effective?
(hint: think about which makes the product fastest)
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4. What
variables
affect enzyme activity in
each of the graphs?
A.
_______________________________
B.
_______________________________
C.
_______________________________
5. What is the
optimal condition
for each
enzyme represented by the graphs? The
optimal condition is when the enzyme would
work best.
A.
_______________________________
B.
_______________________________
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6. What happens when an enzyme gets
too hot
?
7. Explain why graph C
levels off
. Use enzyme and
substrate in your explanation. Why doesn’t it
matter if enzymes keep getting added?
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8. What is the
optimal pH
for both enzymes?
A.
Pepsin _____________
B.
Trypsin_____________
9. Predict the
reactivity
of trypsin at
pH 14
A.
It’s working optimally (best)
B.
The enzyme will be denatured(change shape) and not work
C.
The enzyme will work at a medium rate
10. At what pH do
neither enzyme work
?
11. Which enzyme
works best
at
pH 3
?
12. At what pH values is pepsin likely to be
denatured
?
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Use the graph below to answer questions 13-15.
13. At what
temperature
does this enzyme work
optimally
(best)?
14. What happens to the enzyme activity as the
temperature decreases
from 20
֯
C to 10
֯
C?
15. How can the enzyme activity be
slowed down
?
A.
Increase temperature from 10
֯
C to 20
֯
C
B.
Increase temperature from 20
֯
C to 30
֯
C
C.
Decrease the temperature from 30
֯
C to 20
֯
C
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16.
Humans share several enzymes with thermophilic bacteria.
A.
What is the
temperature
that enzymes work optimally in
humans
?_______
B.
What is the
temperature
that enzymes work optimally in
thermophiles
?_______
17. At what temperature will the enzyme function
for both
human and thermophiles
?
A.
10
֯
C
B.
40
֯
C
C.
50
֯
C
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Based on the graph above:
18. Which enzyme works best in
neutral conditions
?
19. Which enzyme is
not affected
by pH?
20. Which enzyme works under
basic conditions
?
21. Which enzyme might be found in
acidic
stomach
acid?
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The following graph shows data recorded on the effect of
temperature on the functioning of salivary amylase.
21. What is an appropriate
title
for this graph?
22. What is the
independent variable
?
23. What is the
dependent variable
?
24. Based on these results, at what
temperature
does amylase
react the fastest
?
25. What do you think would happen if the
temperature
were
raised above boiling (100
֯
C)?
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The following graph shows data recorded on the
effect of pH on the functioning of salivary amylase.
26. Based on these results, what is the
optimal
(best)
pH
for amylase?
27. Is this
optimal pH
considered acidic, basic, or
neutral?
28. Why does the activity
decrease
when the pH is
too low or too high?
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29. The substrate for a particular reaction is shown below. In
the box labeled “Enzyme,” predict the shape of the enzyme that
will react with this substrate.
Draw the enzyme that would
work with that substrate.
30. Explain the
significance
of the shape of the enzyme that you
just drew.
31. In the box below,
predict
the shape of the
enzyme
AFTER
the reaction has taken place..
Draw it and explain your
prediction.
32. In the box below, predict the shape of the
enzyme
AFTER
the reaction has taken place.
Draw it and explain your
prediction.
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Application:
33. During an illness, like the flu, one may experience very high fevers. How might this
affect the
functioning of enzymes
in the body and the length or time required for recovery?
34. The fur color of the adult northern seal, created by a dark brown pigment, is due to a specific
enzyme.
Suggest reasons why
baby seals are born with a pure white coat.
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1
—
H
o
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s
Answer the questions
based on the graphs.
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Answer the questions based on the graphs in Part I.
35. What is the
function
of an enzyme?
36. What is happening to the
substrate
with enzyme function?
37. Describe the
pattern
of enzyme function shown by the data in the graphs.
38. At which
pH
is the enzyme
functioning
best
? Explain why.
39. If at one pH the substrate is all converted to product in 5 seconds, and at another pH it takes
7 seconds for all of the substrate to be converted, at which
pH
is the enzyme
functioning
best
?
Why?
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2
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H
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Answer the following questions based
on the graphs.
40. Describe the
pattern
of enzyme
function shown by the data in the
graphs.
41. At which
temperature
is the
enzyme
functioning best
? Explain
why.
Extra Question
Looking at graph B, as the enzyme concentration
increases, the rate of reaction ___________.
Increases, decreases, stays the same)
This interactive content provides a detailed exploration of enzyme activity through data interpretation and graph analysis. Questions range from identifying the impact of enzymes on specific molecules to determining optimal conditions for various enzyme functions such as pH and temperature. Users delve into concepts like substrate concentration, enzyme denaturation, and the relationship between graph trends and enzyme effectiveness. The content covers a range of scenarios, from comparing enzyme reactions to understanding optimal pH for different enzymes. Enzyme activity variables, optimal conditions, and practical applications in both humans and thermophilic bacteria are also discussed.
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Presentation Transcript
Enzyme Station Activity INTERPRETING DATA & GRAPHS
Station Station 1 1 1. The enzyme would most likely affect reactions involving A. molecule A, only B. molecule C, only C. molecules B and D D. molecules A and C 2. Which graph, A or B, shows the amount of substrate going to zero faster? 3. If both graphs A and B show the rate of an enzyme, which enzyme is more effective? (hint: think about which makes the product fastest)
Station 2 Station 2 4. What variables affect enzyme activity in each of the graphs? A. _______________________________ B. _______________________________ C. _______________________________ 5. What is the optimal condition for each enzyme represented by the graphs? The optimal condition is when the enzyme would work best. A. _______________________________ B. _______________________________
Station Station 2 2 6. What happens when an enzyme gets too hot? 7. Explain why graph C levels off. Use enzyme and substrate in your explanation. Why doesn t it matter if enzymes keep getting added?
Station Station 3 3 8. What is the optimal pH for both enzymes? A. Pepsin _____________ B. Trypsin_____________ 9. Predict the reactivity of trypsin at pH 14 A. B. C. It s working optimally (best) The enzyme will be denatured(change shape) and not work The enzyme will work at a medium rate 10. At what pH do neither enzyme work? 11. Which enzyme works best at pH 3? 12. At what pH values is pepsin likely to be denatured?
Station Station 4 4 Use the graph below to answer questions 13-15. 13. At what temperature does this enzyme work optimally (best)? 14. What happens to the enzyme activity as the temperature decreases from 20 C to 10 C? 15. How can the enzyme activity be slowed down? A. Increase temperature from 10 C to 20 C B. Increase temperature from 20 C to 30 C C. Decrease the temperature from 30 C to 20 C
Station 5 Station 5 16. Humans share several enzymes with thermophilic bacteria. A. What is the temperature that enzymes work optimally in humans?_______ B. What is the temperature that enzymes work optimally in thermophiles?_______ 17. At what temperature will the enzyme function for both human and thermophiles? A. 10 C B. 40 C C. 50 C
Station Station 6 6 Based on the graph above: 18. Which enzyme works best in neutral conditions? 19. Which enzyme is not affected by pH? 20. Which enzyme works under basic conditions? 21. Which enzyme might be found in acidic stomach acid?
Station Station 7 7 The following graph shows data recorded on the effect of temperature on the functioning of salivary amylase. 21. What is an appropriate title for this graph? 22. What is the independent variable? 23. What is the dependent variable? 24. Based on these results, at what temperature does amylase react the fastest? 25. What do you think would happen if the temperature were raised above boiling (100 C)?
Station Station 8 8 The following graph shows data recorded on the effect of pH on the functioning of salivary amylase. 26. Based on these results, what is the optimal (best) pH for amylase? 27. Is this optimal pH considered acidic, basic, or neutral? 28. Why does the activity decrease when the pH is too low or too high?
Station 9 Station 9 29. The substrate for a particular reaction is shown below. In the box labeled Enzyme, predict the shape of the enzyme that will react with this substrate. Draw the enzyme that would work with that substrate. 30. Explain the significance of the shape of the enzyme that you just drew. 31. In the box below, predict the shape of the enzyme AFTER the reaction has taken place.. Draw it and explain your prediction. 32. In the box below, predict the shape of the enzyme AFTER the reaction has taken place. Draw it and explain your prediction.
Station Station 10 10 Application: 33. During an illness, like the flu, one may experience very high fevers. How might this affect the functioning of enzymes in the body and the length or time required for recovery? 34. The fur color of the adult northern seal, created by a dark brown pigment, is due to a specific enzyme. Suggest reasons why baby seals are born with a pure white coat.
Station Station 11 Honors Honors 11 Answer the questions based on the graphs.
Station Station 11 11 Honors Honors Answer the questions based on the graphs in Part I. 35. What is the function of an enzyme? 36. What is happening to the substrate with enzyme function? 37. Describe the pattern of enzyme function shown by the data in the graphs. 38. At which pH is the enzyme functioning best? Explain why. 39. If at one pH the substrate is all converted to product in 5 seconds, and at another pH it takes 7 seconds for all of the substrate to be converted, at which pH is the enzyme functioning best? Why?
Station 12 Station 12 Honors Honors Answer the following questions based on the graphs. 40. Describe the pattern of enzyme function shown by the data in the graphs. 41. At which temperature is the enzyme functioning best? Explain why.
Extra Question Looking at graph B, as the enzyme concentration increases, the rate of reaction ___________. Increases, decreases, stays the same)
