Free Science Education Website - Science Prof Online PowerPoint Resources
AboutScience Prof Online
PowerPoint Resources
•
Science Prof Online (SPO) is a free science education website that provides fully-developed Virtual Science Classrooms,
science-related PowerPoints, articles and images. The site is designed to be a helpful resource for students, educators, and
anyone interested in learning about science.
•
The SPO Virtual Classrooms offer many educational resources, including practice test questions, review questions, lecture
PowerPoints, video tutorials, sample assignments and course syllabi. New materials are continually being developed, so check
back frequently, or follow us on Facebook (Science Prof Online) or Twitter (ScienceProfSPO) for updates.
•
Many SPO PowerPoints are available in a variety of formats, such as fully editable PowerPoint files, as well as uneditable
versions in smaller file sizes, such as PowerPoint Shows and Portable Document Format (.pdf), for ease of printing.
•
Images used on this resource, and on the SPO website are, wherever possible, credited and linked to their source. Any
words underlined and appearing in blue are links that can be clicked on for more information. PowerPoints must be viewed in
slide show mode
to use the hyperlinks directly.
•
Several helpful links to fun and interactive learning tools are included throughout the PPT and on the Smart Links slide,
near the end of each presentation. You must be in
slide show mode
to utilize hyperlinks and animations.
•
This digital resource is licensed under Creative Commons
Attribution-ShareAlike 3.0:
Alicia Cepaitis, MS
Chief Creative Nerd
Science Prof Online
Online Education Resources, LLC
Image: Compound microscope objectives, T. Port
Tami Port, MS
Creator of Science Prof Online
Chief Executive Nerd
Science Prof Online
Online Education Resources, LLC
From the Virtual Microbiology Classroom onScienceProfOnline.com
Laboratory
LAB MEETS 1x WEEKLY
•
Be on time. 10 minutes after the start of lab, the doors may be locked.
LAB REQUIREMENTS:
•
Y
ou must have the following to participate in lab:
- Goggles
(We have those in lab for you)
- Lab coat
(We have those in lab for you)
-
Shoes that completely cover feet, long pants
(No shorts or capris),
&
long hair up
.
LAB QUIZ & REPORT:
•
Each week there will be an open-book lab quiz available on Moodle. It must be completed before lab. The quiz
will cover information from that week’s lab (i.e. the upcoming lab). This is how I assess whether or not you are
prepared to safely participate in lab.
•
Each Lab Project has an associated Lab Report. See Moodle syllabus for schedule of due dates for homework.
WHERE IS THE LAB MANUAL?
•
The links to the Laboratory material are on Moodle.
IT IS NOT ALWAYS POSSIBLE TO MAKE UP LABORATORY SESSIONS
:
Image: : The Muppets Beaker & Dr. Bunsen Honeydew
From the Virtual Microbiology Classroom onScienceProfOnline.com
Laboratory
Exercise 1
How to Use a
Compound Microscope
Basic Microscopy
Image: The Far Side by Gary Larson
From the Virtual Microbiology Classroom on
ScienceProfOnline.com
What am I going to learn from Lab Topic #1?
•
How to make a
wet mount slide
(Sweeeet)
!
•
How to microscopically view wet
mounted
plant cells
&
animal cells
.
•
How to use and maintain a
compound light microscope
.
•
How to protect yourself from
other people’s bodily fluids
.
Image:
Chimp brain in a jar
, Gaetan Lee
From the Virtual Microbiology Classroom on
ScienceProfOnline.com
Anton van Leeuwenhoek’s
“
Animalcules
”
(Pronounced Lay-
v
en-hook)
As a
draper
(merchant who sells cloth and
dry goods)
, he used lenses to examine
cloth. This probably led to his
interest in lens making.
He assembled hundreds of
microscopes, some of which
magnified objects 270 times.
As he looked at things with his
microscopes, he discovered
“
micro
”
organisms - organisms so tiny that
they were invisible to the naked
eye.
He called these tiny living
organisms
“
animalcules
”
. He first
described bacteria, protozoans and
many cells of the human body.
Images:
Leeuwenhoek portrait
1680, Jan Verkolje
(I);.
Replica of Leeuwenhoek microscope
, Jacopo
Werther; Man with scope, source unknown
1632 - 1723
From the Virtual Biology Classroom on
ScienceProfOnline.com
Compound Light Microscope
The “Compound” Part
•
Simple microscopes
have single magnifying
lens
(like a
magnifying glass).
•
Compound microscopes
have
two sets of lenses
for
magnification.
•
Lens closer to the eye =
ocular lens
(magnifying power of 10x).
•
Lenses closer to the object being viewed =
objective
lens
.
(Most light microscopes used in biology have three or four
objective lenses).
The “Light” Part
•
Bri
ght-field light
microscopes produce a dark image
against brighter, backlit background.
•
Provide a 2-D image.
•
Commonly used to view stained cells.
Image:
Magnifying lamp
use to look for part defects,
US Navy;
Compound light microscope
, Moisey
From the Virtual Microbiology Classroom on
ScienceProfOnline.com
Your Microscope
Take Care of Your Scope:
•
It is
your responsibility
to take care of your scope and learn to use
it properly.
•
I randomly check scopes to see if they are put away correctly. If
yours is not, I may subtract points from your lab grade.
Microscope Log:
•
If you get your scope out and find that it has not been put away
properly, make an entry in the
microscope log.
Getting Scope Out:
•
The scope that you use is numbered to correspond to your seat
number, on edge of lab bench in front of you.
•
When transporting your scope, always hold it with one hand under
the
base
, and one hand around the
arm
.
Putting Scope Away:
•
Clean stage if it is oily, and use lens paper to clean lenses.
•
Shortest objective lens
(the one with the red band)
should be pointing
down toward stage.
•
Use course focus to position stage
as low as it can go
.
•
Always put scope back in numbered parking spot in scope cabinet.
*
*
N
o
w
e
v
e
r
y
o
n
e
g
e
t
o
u
t
t
h
e
i
r
s
c
o
p
e
*
*
B
A
S
E
A
R
M
Image:
Compound light microscope
, Moisey
From the Virtual Microbiology Classroom on
ScienceProfOnline.com
Parts of a Compound Light
Microscope
Image:
Compound light microscope
, Moisey
From the Virtual Microbiology Classroom on
ScienceProfOnline.com
Ocular lens magnifies the
specimen 10x.
You will always be looking
through the ocular and
objective lens simultaneously,
so multiply ocular magnification
x objective power to calculate
the Total Magnification (xTM).
Rotary nosepiece of your
microscope has four objective
lenses attached.
Shortest lens
(red band)
should
have been pointing down when
your scopes were last put away.
Magnification & The Objective Lenses
Image:
Microscope objective lenses, T. Port
From the Virtual Microbiology Classroom on
ScienceProfOnline.com
Scanning Power Objective Lens
Image:
Microscope objective lenses, T. Port
•
Red band around it.
•
Magnifies objects 4x.
•
Q:
What is the Total
Magnification?
____ TM
•
We will only use this lens in
today’s lab. It is not useful
for looking at bacteria.
From the Virtual Microbiology Classroom on
ScienceProfOnline.com
Low Power Objective Lens
•
Has yellow band around it.
•
Magnifies objects 10x.
•
Q:
What is the Total
Magnification?
____ TM
•
Start with this lens when
looking at a bacterial smear.
•
Q:
What does the term
parfocal
mean?
Image:
Microscope objective lenses, T. Port
From the Virtual Microbiology Classroom on
ScienceProfOnline.com
High Dry Objective Lens
•
Has blue band around it.
•
Magnifies objects 40x.
•
Q:
What is the Total
Magnification?
____ TM
•
Switch to this lens after
getting your specimen in focus
at 100xTM.
Image:
Microscope objective lenses, T. Port
From the Virtual Microbiology Classroom on
ScienceProfOnline.com
•
Many microscopic images in your
textbook were obtained using
electron microscopes
.
•
Electron beam wavelengths are
shorter than light wavelengths,
so better resolving power.
•
Q:
What is
resolution
, in the
context of microscopy?
•
Transmission Electron
Microscope (TEM):
2-D image
•
Scanning Electron Microscope
(SEM):
3-D image
Microscopy
:
Electron Microscopes
Images:
Poliovirus, taken with TEM,
PHIL
#1875,
Blood cells
, taken
with SEM, National Cancer Institute; Transmission electron
microscope, Public Health Image Library, image #14236.
From the Virtual Microbiology Classroom on
ScienceProfOnline.com
How to make
a wet mount
Image: Wet mount procedure
, T. Port
From the Virtual Microbiology Classroom on
ScienceProfOnline.com
P
r
o
c
e
d
u
r
e
1.
How to make a wet mount
2. Letter “e”
-
What happens to the “e” when you look at it
through the lens?
-
What happens to your view of the “e” when
your increase magnification?
3. Onion
-
Wet mount, use stain
-
Note nucleus and cell wall
-
View video tutorial
4.
Elodea
-
Wet mount NO stain
- Depth of field
-
2 layers of cells
-
Note cell wall, chloroplasts streaming
-
View video tutorial
5. Cheek cell
-
Wet mount using NaCl & Methylene blue.
-
Contrast!
-
View video tutorial
Images:
Onion cells, Elodea cells & Cheek cells,
SPO General
Microbiology Microscopic Images
;
Elodea plant
, Frank Vincentz
From the Virtual Microbiology Classroom on
ScienceProfOnline.com
Confused?
Here are links to fun resources that further explain use of
the microscope:
•
Microscopy Laboratory
Main Page
on the Virtual Microbiology Classroom
of
Science Prof Online
.
•
Compound Microscope Parts and Use
video
from Science ProfOnline.
•
How to Make a Wet Mount of an Elodea Plant Cell
video from
ScienceProfOnline.
•
How to Make a Wet Mount of an Onion Epithelial Cell
video
from ScienceProfOnline.
•
How to Make a Wet Mount of a Cheek Cell
video from
ScienceProfOnline.
•
Play
Amoeba
,
a video game where you are an amoeba that eats and grows.
•
Microscope Mania
crossword puzzle.
•
Microscopic Pond Life
, an extremely cool collection of videos of a variety of
microscopic pond life to the tune of Radiohead’s “Kid A”.
(You must be in PPT slideshow view to click on links.)
From the Virtual Microbiology Classroom on
ScienceProfOnline.com
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:
•
practice test questions
•
review questions
•
study guides and learning objectives
You can access the VMC by going to the Science Prof Online website
www.ScienceProfOnline.com
Images:
White blood cell
, Giant Microbes;
Prokaryotic cell
, Mariana Ruiz
Welcome to Science Prof Online PowerPoint Resources!
This PowerPoint Presentation comes from the Virtual Cell Biology Classroom of Science Prof Online, and, as such, is licensed under Creative Commons Attribution-ShareAlike 3.0.; meaning you can download, share and alter any of this presentation, but you can’t sell it or repackage and sell any part of it. Please credit Science Prof Online as the source of this presentation. Please abide by credited image copyrights. Thank you for using this resource.
Science Prof Online (SPO) is a free science education website offering fully-developed Virtual Science Classrooms, science-related PowerPoints, articles, and images. This resource is designed to assist students, educators, and anyone interested in learning about science. The site provides a wide range of educational resources such as practice test questions, lecture PowerPoints, video tutorials, and sample assignments. Stay updated with new materials by following SPO on social media. The PowerPoints are available in various formats for easy access and printing. Explore interactive learning tools and engage with fun learning resources throughout the presentations.
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About Science Prof Online PowerPoint Resources Science Prof Online (SPO) is a free science education website that provides fully-developed Virtual Science Classrooms, science-related PowerPoints, articles and images. The site is designed to be a helpful resource for students, educators, and anyone interested in learning about science. The SPO Virtual Classrooms offer many educational resources, including practice test questions, review questions, lecture PowerPoints, video tutorials, sample assignments and course syllabi. New materials are continually being developed, so check back frequently, or follow us on Facebook (Science Prof Online) or Twitter (ScienceProfSPO) for updates. Many SPO PowerPoints are available in a variety of formats, such as fully editable PowerPoint files, as well as uneditable versions in smaller file sizes, such as PowerPoint Shows and Portable Document Format (.pdf), for ease of printing. Images used on this resource, and on the SPO website are, wherever possible, credited and linked to their source. Any words underlined and appearing in blue are links that can be clicked on for more information. PowerPoints must be viewed in slide show mode to use the hyperlinks directly. Several helpful links to fun and interactive learning tools are included throughout the PPT and on the Smart Links slide, near the end of each presentation. You must be in slide show mode to utilize hyperlinks and animations. This digital resource is licensed under Creative Commons Attribution-ShareAlike 3.0: http://creativecommons.org/licenses/by-sa/3.0/ Tami Port, MS Creator of Science Prof Online Chief Executive Nerd Science Prof Online Online Education Resources, LLC info@scienceprofonline.com Alicia Cepaitis, MS Chief Creative Nerd Science Prof Online Online Education Resources, LLC alicia@scienceprofonline.com From the Virtual Microbiology Classroom on ScienceProfOnline.com Image: Compound microscope objectives, T. Port
Laboratory LAB MEETS 1x WEEKLY Be on time. 10 minutes after the start of lab, the doors may be locked. LAB REQUIREMENTS: You must have the following to participate in lab: - Goggles (We have those in lab for you) - Lab coat (We have those in lab for you) - Shoes that completely cover feet, long pants(No shorts or capris), & long hair up. LAB QUIZ & REPORT: Each week there will be an open-book lab quiz available on Moodle. It must be completed before lab. The quiz will cover information from that week s lab (i.e. the upcoming lab). This is how I assess whether or not you are prepared to safely participate in lab. Each Lab Project has an associated Lab Report. See Moodle syllabus for schedule of due dates for homework. WHERE IS THE LAB MANUAL? The links to the Laboratory material are on Moodle. IT IS NOT ALWAYS POSSIBLE TO MAKE UP LABORATORY SESSIONS: From the Virtual Microbiology Classroom on ScienceProfOnline.com Image: : The Muppets Beaker & Dr. Bunsen Honeydew
Laboratory Exercise 1 How to Use a Compound Microscope Basic Microscopy From the Virtual Microbiology Classroom on ScienceProfOnline.com Image: The Far Side by Gary Larson
What am I going to learn from Lab Topic #1? How to make a wet mount slide (Sweeeet)! How to microscopically view wet mounted plant cells & animal cells. How to use and maintain a compound light microscope. How to protect yourself from other people s bodily fluids . From the Virtual Microbiology Classroom on ScienceProfOnline.com Image: Chimp brain in a jar, Gaetan Lee
Anton van Leeuwenhoeks Animalcules (Pronounced Lay-ven-hook) As a draper(merchant who sells cloth and dry goods), he used lenses to examine cloth. This probably led to his interest in lens making. 1632 - 1723 He assembled hundreds of microscopes, some of which magnified objects 270 times. As he looked at things with his microscopes, he discovered micro organisms - organisms so tiny that they were invisible to the naked eye. He called these tiny living organisms animalcules . He first described bacteria, protozoans and many cells of the human body. Everett showing how to use the microscope Images: Leeuwenhoek portrait 1680, Jan Verkolje (I);. Replica of Leeuwenhoek microscope, Jacopo Werther; Man with scope, source unknown From the Virtual Biology Classroom on ScienceProfOnline.com
Compound Light Microscope The Compound Part Simple microscopes have single magnifyinglens (like a magnifying glass). Compound microscopes have two sets of lenses for magnification. Lens closer to the eye = ocular lens (magnifying power of 10x). Lenses closer to the object being viewed = objective lens. (Most light microscopes used in biology have three or four objective lenses). The Light Part Bright-field light microscopes produce a dark image against brighter, backlit background. Provide a 2-D image. Commonly used to view stained cells. Image: Magnifying lamp use to look for part defects, US Navy; Compound light microscope, Moisey From the Virtual Microbiology Classroom on ScienceProfOnline.com
Your Microscope Take Care of Your Scope: It is your responsibility to take care of your scope and learn to use it properly. I randomly check scopes to see if they are put away correctly. If yours is not, I may subtract points from your lab grade. ARM Microscope Log: If you get your scope out and find that it has not been put away properly, make an entry in the microscope log. Getting Scope Out: The scope that you use is numbered to correspond to your seat number, on edge of lab bench in front of you. When transporting your scope, always hold it with one hand under the base, and one hand around the arm. Putting Scope Away: Clean stage if it is oily, and use lens paper to clean lenses. Shortest objective lens (the one with the red band) should be pointing down toward stage. Use course focus to position stage as low as it can go. Always put scope back in numbered parking spot in scope cabinet. BASE ** Now everyone get out their scope ** From the Virtual Microbiology Classroom on ScienceProfOnline.com Image: Compound light microscope, Moisey
Parts of a Compound Light Microscope From the Virtual Microbiology Classroom on ScienceProfOnline.com Image: Compound light microscope, Moisey
Magnification & The Objective Lenses Ocular lens magnifies the specimen 10x. You will always be looking through the ocular and objective lens simultaneously, so multiply ocular magnification x objective power to calculate the Total Magnification (xTM). Rotary nosepiece of your microscope has four objective lenses attached. Shortest lens (red band) should have been pointing down when your scopes were last put away. From the Virtual Microbiology Classroom on ScienceProfOnline.com Image: Microscope objective lenses, T. Port
Scanning Power Objective Lens Red band around it. Magnifies objects 4x. Q: What is the Total Magnification? ____ TM We will only use this lens in today s lab. It is not useful for looking at bacteria. From the Virtual Microbiology Classroom on ScienceProfOnline.com Image: Microscope objective lenses, T. Port
Low Power Objective Lens Has yellow band around it. Magnifies objects 10x. Q: What is the Total Magnification? ____ TM Start with this lens when looking at a bacterial smear. Q: What does the term parfocal mean? From the Virtual Microbiology Classroom on ScienceProfOnline.com Image: Microscope objective lenses, T. Port
High Dry Objective Lens Has blue band around it. Magnifies objects 40x. Q: What is the Total Magnification? ____ TM Switch to this lens after getting your specimen in focus at 100xTM. From the Virtual Microbiology Classroom on ScienceProfOnline.com Image: Microscope objective lenses, T. Port
Microscopy:Electron Microscopes Many microscopic images in your textbook were obtained using electron microscopes. Electron beam wavelengths are shorter than light wavelengths, so better resolving power. Q: What is resolution, in the context of microscopy? Transmission Electron Microscope (TEM): 2-D image Scanning Electron Microscope (SEM): 3-D image Images: Poliovirus, taken with TEM, PHIL #1875, Blood cells, taken with SEM, National Cancer Institute; Transmission electron microscope, Public Health Image Library, image #14236. From the Virtual Microbiology Classroom on ScienceProfOnline.com
How to make a wet mount From the Virtual Microbiology Classroom on ScienceProfOnline.com Image: Wet mount procedure, T. Port
Procedure 1. How to make a wet mount 2. Letter e - What happens to the e when you look at it through the lens? - What happens to your view of the e when your increase magnification? 3. Onion - Wet mount, use stain - Note nucleus and cell wall - View video tutorial 4.Elodea - Wet mount NO stain - Depth of field - 2 layers of cells - Note cell wall, chloroplasts streaming - View video tutorial 5. Cheek cell - Wet mount using NaCl & Methylene blue. - Contrast! - View video tutorial Images: Onion cells, Elodea cells & Cheek cells, SPO General Microbiology Microscopic Images; Elodea plant, Frank Vincentz From the Virtual Microbiology Classroom on ScienceProfOnline.com
Confused? Here are links to fun resources that further explain use of the microscope: Microscopy Laboratory Main Pageon the Virtual Microbiology Classroom of Science Prof Online. Smart Links Compound Microscope Parts and Use videofrom Science ProfOnline. How to Make a Wet Mount of an Elodea Plant Cell video from ScienceProfOnline. How to Make a Wet Mount of an Onion Epithelial Cell video from ScienceProfOnline. How to Make a Wet Mount of a Cheek Cell video from ScienceProfOnline. Play Amoeba, a video game where you are an amoeba that eats and grows. Microscope Mania crossword puzzle. Microscopic Pond Life, an extremely cool collection of videos of a variety of microscopic pond life to the tune of Radiohead s Kid A . From the Virtual Microbiology Classroom on ScienceProfOnline.com (You must be in PPT slideshow view to click on links.)
Are microbes intimidating you? Do yourself a favor. Use the Virtual Microbiology Classroom (VMC) ! The VMC is full of resources to help you succeed, including: practice test questions review questions study guides and learning objectives You can access the VMC by going to the Science Prof Online website www.ScienceProfOnline.com Images: White blood cell, Giant Microbes; Prokaryotic cell, Mariana Ruiz
