Characterizing Blot Patterns in WT and SMA1 Cell Lines
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A.
Branching
B.
Polarity
C.
Van der Waal interactions
D.
H-bonding
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a)
When the pressure is less than 1 atm , the boiling point of the
liquid is less than its normal b.p.
b)
When P = 1 atm, the b.p. of the liquid is called normal boiling
point
c)
When P is greater than 1 atm, the b.p. of the liquid is greater
than its normal b.p.
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By increasing Molecular weight b.p increase.
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A solution has a higher boiling point than a pure solvent .
The b.p. of pure H
2
O is 100
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C, but that b.p. can be
elevated by adding a solute such as a salt.
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Within the branched series, increased branch leads to lower boiling
point.
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Polarity raises b.p.
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As the number of carbon in an alkanes increase, the boiling point increase due
to the larger surface area and the van der walls interactions increased.
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Analyzing blot images showing protein expression levels in WT and SMA1 cell lines, focusing on Beta-actin and SMN proteins. The unedited blots provide insights into the comparison of protein levels between the different samples. These results contribute to understanding the molecular differences in WT and SMA1 cells.
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Presentation Transcript
Boiling Point Determination
Definition Boiling point is the temperature at which the vapor pressure of the liquid is equal to the atmospheric pressure and change its state from a liquid to a gas. In terms of intermolecular interactions the b.p. represents the energy required to overcome the various intermolecular attractions binding the molecules as a liquid (e.g. dipole-dipole attraction and hydrogen bonding). Therefore the boiling point of a liquid is also an indicator of the strength of the attractive forces between the liquid s molecules.
factors affecting boiling point (b.p) 1)Pressure 2)Molecular weight 3)Impurities 4)Structure of the molecule & intermolecular interactions like A. Branching B. Polarity C. Van der Waal interactions D. H-bonding
factors affecting boiling point (b.p) 1- Pressure a) When the pressure is less than 1 atm , the boiling point of the liquid is less than its normal b.p. b) When P = 1 atm, the b.p. of the liquid is called normal boiling point c) When P is greater than 1 atm, the b.p. of the liquid is greater than its normal b.p.
factors affecting boiling point (b.p) 2- Molecular weight By increasing Molecular weight b.p increase.
factors affecting boiling point (b.p) 3- Impurities A solution has a higher boiling point than a pure solvent . The b.p. of pure H2O is 100 oC, but that b.p. can be elevated by adding a solute such as a salt.
factors affecting boiling point (b.p) 4-Structure of the molecule & intermolecular interactions 1-Branching Within the branched series, increased branch leads to lower boiling point.
factors affecting boiling point (b.p) 4-Structure of the molecule & intermolecular interactions 2-Polarity Polarity raises b.p.
factors affecting boiling point (b.p) 4-Structure of the molecule & intermolecular interactions 3-Van der Waal interactions As the number of carbon in an alkanes increase, the boiling point increase due to the larger surface area and the van der walls interactions increased.
factors affecting boiling point (b.p) 4-Structure of the molecule & intermolecular interactions 4- Hydrogen bonding
