Explore various physical and chemical methods used for gene transfer, including electroporation, particle bombardment, microinjection, ultrasound, and bacterial transformation. Learn how DNA enters the nucleus and the role of retroviruses in gene transfer efficiency. Understand the process of RNA co

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HER2 activation plays a crucial role in promoting tumor proliferation and survival in NSCLC. Driven by oncogenic downstream signaling pathways, HER2 overexpression and gene amplification lead to the formation of heterodimers and activation of key signaling cascades. Additionally, HER2 mutations are

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This article provides insights into the status, regulatory considerations, and delivery methods of gene therapy and genome editing technologies. It discusses approved gene therapies in the US, human genome editing in clinical applications, and the regulatory authority of the FDA over gene therapy. T

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This slideshow provides an overview of tumor classification, nomenclature, and key concepts in pathology. It covers the definitions of neoplasm, tumor, and oncology, the classification of tumors into benign and malignant categories, as well as the importance of stroma in tumor behavior. It also expl

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Gene expression is a crucial process that leads to the production of functional gene products like RNA and proteins. This article explores the regulation of gene expression in both prokaryotes and eukaryotes, covering the importance of regulating gene expression, types of regulation elements, specif

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Gene set enrichment tests help identify functional gene sets enriched in hit lists compared to background sets. Various biases (technical, biological, statistical) can lead to incorrect conclusions in data analysis, emphasizing the importance of recognizing and addressing them. Technical biases like

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Delve into the world of genetics and genomics through the lens of integrative biology, investigating the differences between cell types, the rationale for gene expression profiling, and the analysis of differential gene expression in various diseases. Uncover the significance of gene ontology, co-ex

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This content provides information on the Summer Institutes of Statistical Genetics module, focusing on gene expression profiling. It includes details on the schedule, experimental design, RNA sequencing workflow, modes of bulk RNA sequencing, and RNASeq software. The content discusses crucial steps

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Gene mutations play a significant role in molecular biology, leading to alterations in DNA sequences that can impact offspring. These mutations can arise spontaneously or be induced by various factors, such as mutagens. Understanding gene mutations is crucial for comprehending the genetic basis of c

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WAGR syndrome is a rare genetic condition associated with Wilms Tumor, aniridia, and developmental delays. Wilms Tumor is a form of kidney cancer mostly affecting children and is linked to genetic disorders like WAGR syndrome. The risk of Wilms Tumor is higher in children with WAGR syndrome, with po

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Uganda has successfully implemented an Assisted Partner Notification (APN) program to reach men for HIV testing, addressing the gender gap in testing rates. By utilizing various approaches such as index testing, self-testing, and social network testing, Uganda has achieved significant success in tar

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Gene drive technology is a cutting-edge approach aimed at combating malaria by spreading genetically modified genes in mosquito populations. This technology has the potential to protect endangered species and conserve nature. However, successful implementation requires complex collaborations, risk a

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Gene therapy involves introducing DNA into a patient to treat genetic diseases by correcting disease-causing mutations. There are two types of gene therapy: somatic gene therapy and germline gene therapy, each with different implications. Techniques such as gene augmentation therapy and gene inhibit

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Explore key concepts in software testing throughout the Software Development Lifecycle (SDLC) with a quiz covering topics like white-box testing in acceptance testing, component testing vs. system testing, and regression testing purposes. Enhance your understanding of testing methodologies with samp

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Software testing is crucial in ensuring the reliability and safety of software systems, as highlighted by catastrophic failures such as the Ariane 5 rocket incident and the Therac-25 radiation therapy machine disasters. These examples underscore the importance of thorough testing in identifying and

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Research collaboration aimed to enhance locoregional tumor control and reduce distant failure by combining charged particles irradiation and anticancer drug Epothilone B in cultured human tumor cells from Milano and Roma. The study explores the impact on clonogenic survival, growth curves, and deter

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Major effort is focused on verification and testing in SCREAM for the E3SM Fall All-Hands. The initiative includes unit testing, property testing, regression testing, and leveraging various tools like Cmake, Python, Jenkins, AutoTester, and GitHub for Continuous Integration (CI). The emphasis is on

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This comprehensive content delves into the intricate mechanisms of gene regulation and control of gene expression. It covers topics such as transcriptional regulation, bacterial genes classification, and the role of regulatory proteins. Explore how genes are regulated at transcription, translation,

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Explore the dynamic world of gene expression with the Chunky and Chips Gene Expression Group at the University of Notre Dame. Dive into topics like transcription, translation, and cellular events influencing gene expression. Delve into real-world scenarios and gain insights into the cellular level b

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NCI-MATCH is a groundbreaking clinical trial aiming to match tumor gene alterations with targeted therapies. The interim analysis results revealed impressive patient enrollment and site participation exceeding expectations. Despite challenges with pausing new patient registrations for the analysis,

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Epistasis is a phenomenon where the phenotypic expression of one gene is influenced by interactions with another gene. This concept, first introduced in 1909, plays a crucial role in genetics, affecting various traits and evolutionary processes. The difference between dominance and epistasis lies in

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Vestibular schwannoma, also known as acoustic neuroma, is a common tumor in the cerebellopontine angle. It can be sporadic or associated with neurofibromatosis type 2. Patients typically present with hearing loss and tinnitus, which may be gradual or sudden. Grading systems like Koos, Ojemann, and J

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The Gene Wiki Jamboree FaceBase Spring Meeting in 2010 aimed to create collaboratively-written and community-reviewed review articles for every gene in the human genome and every concept relevant to FaceBase. It highlighted the value of such articles, providing quick summaries and compilations of re

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In the previous session, we discussed various aspects of software engineering, including modeling with UML diagrams, such as activity diagrams, use case diagrams, sequence diagrams, state diagrams, and class diagrams, as well as architecture patterns. Testing was emphasized as a key aspect, highligh

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This supplementary data includes raw data from agarose gel electrophoresis showing gene expression levels for human SOX17, FOXA2, AFP, ALB, and ACTB in various cellular samples. The gel images depict the expected sizes of amplicons for each gene in different conditions and time points of cell cultur

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Gray Box Testing is a software testing technique that involves testing the software with partial knowledge of its internal workings. It combines aspects of White Box Testing and Black Box Testing, allowing testers to check both the presentation layer and the code part of an application. Gray Box Tes

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In this project, the focus is on identifying splice sites relevant to the inhibitory dopamine receptor gene (D2). The problem involves finding splicing elements within a specific range of known splice sites in the gene. The data provided includes the D2 gene entry in FASTA format, a database of spli

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This tutorial, prepared by the International Working Group for TIL in breast cancer, provides guidelines for pathologists on the standardized evaluation of tumor-infiltrating lymphocytes (TILs) in breast cancer based on H&E slides of core biopsies or tumor resections. It details steps such as defini

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Reverse genetic approaches involve mutating a target gene to observe its phenotypic effects, providing insights into the gene's normal function. Techniques like gene knockout, knockdown, and homologous recombination are used to manipulate genes and study genetic pathways, leading to discoveries in v

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The lecture covers the essential aspects of hypothesis testing in gene expression profiling, emphasizing experimental design, confounding factors, normalization of samples, linear modeling, gene-level contrasts, t-tests, ANOVA, and significance assessment techniques. Practical insights are shared on

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Exploring the fundamentals of genetics engineering, this lecture covers topics such as DNA structure, gene expression, gene function, genome, mutation, recombination, and genetic modification. Understand the significance of DNA, genes, and the processes involved in gene expression. Dive into the wor

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Expression vectors are designed to facilitate gene expression by carrying essential elements such as promoters, terminators, and fusion tags. They allow precise control over gene regulation for transcription and translation in host cells, enabling efficient protein production. Different promoters li

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Cancer's complex evolution introduces challenges in treatment response. B-SCITE aims to enhance tumor phylogeny inference by integrating bulk sequencing and single-cell data using a probabilistic approach. It addresses the complexity of tumor cell populations and potential treatment failure causes.

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These guidelines recommend HIV testing during pregnancy, at delivery, and postpartum. Testing should be done early in pregnancy and again in the third trimester. Expedited testing during labor is required for certain patients, and syphilis testing is recommended. Pre-exposure and post-exposure proph

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In this project, the Cstps-1 gene from Citrus sinensis is being cloned to produce the enzyme Citrus Valencene Synthase (CVS) responsible for generating the fragrant valencene compound. The process involves DNA isolation from a Valencia orange, gene amplification, vector transformation, and testing f

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Multiple enhancers play a crucial role in regulating gene expression in a cell, with approximately 3-5 enhancers associated per human gene. These enhancers are essential for tissue-specific expressions and are active within the same cell line. The use of multiple enhancers can impact gene expression

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Concept Software is a discipline comprising various code pieces. Testing these codes together is complex but vital in Software Engineering. The process includes early testing like unit tests, pairwise/multiple component testing, module testing, integration testing, user tests, alpha tests, beta test

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Workshop outcomes and discussions from the MDA-NINDS Workshop on Best Practices for Gene Therapy Programs, highlighting key challenges, possible solutions, and the impact on translational funding programs. The workshop format included orienting talks and panels focusing on scientific premise, regula

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Cr(VI)-induced mutagenesis is a key event in the mode of action for small intestinal tumors in mice, with the question of whether it is an essential step in tumor development. The interaction of cellular components with Cr leads to cell proliferation, hyperplasia, and ultimately mutagenesis. Differe

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Understanding the critical role of software and system testing in identifying and fixing errors before they lead to major failures. Various types of testing such as functional, usability, performance, and reliability testing are essential to ensure the quality of software products. Different classif

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