Gene manipulation - PowerPoint PPT Presentation


Overview of Gene Transfer Methods in Molecular Biology

Explore various physical and chemical methods used for gene transfer, including electroporation, particle bombardment, microinjection, ultrasound, and bacterial transformation. Learn how DNA enters the nucleus and the role of retroviruses in gene transfer efficiency. Understand the process of RNA co

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Gene Therapy and Genome Editing Technology

This article provides insights into the status, regulatory considerations, and delivery methods of gene therapy and genome editing technologies. It discusses approved gene therapies in the US, human genome editing in clinical applications, and the regulatory authority of the FDA over gene therapy. T

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Understanding Viral Vectors in Gene Delivery: Safety and Considerations

Exploring the mechanisms of gene delivery via viral vectors, this content delves into the basics of viral structure, infection, and replication. It discusses common viral vector systems like Adenoviral and Lentiviral vectors, highlighting safety considerations and the production processes involved.

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Understanding Gene Editing with CRISPR/Cas9 Technology

Explore the fascinating world of CRISPR/Cas9 gene editing technology through sessions led by experts like Roy Campbell and Howard Robinson. Delve into the working of gene editing, the CRISPR system, and the role of Cas9 enzyme. Learn about CRISPR's origin in prokaryotic organisms and its potential f

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Understanding Gene Expression Regulation in Prokaryotes and Eukaryotes

Gene expression is a crucial process that leads to the production of functional gene products like RNA and proteins. This article explores the regulation of gene expression in both prokaryotes and eukaryotes, covering the importance of regulating gene expression, types of regulation elements, specif

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Bioinformatics

Bioinformatics involves analyzing biological sequences through sequence alignment to uncover functional, structural, and evolutionary insights. This process helps in tasks like annotation of sequences, modeling protein structures, and analyzing gene expression experiments. Basic steps include compar

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Understanding Artefacts and Biases in Gene Set Analysis

Gene set enrichment tests help identify functional gene sets enriched in hit lists compared to background sets. Various biases (technical, biological, statistical) can lead to incorrect conclusions in data analysis, emphasizing the importance of recognizing and addressing them. Technical biases like

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Exploring Genetics and Genomics in Integrative Biology

Delve into the world of genetics and genomics through the lens of integrative biology, investigating the differences between cell types, the rationale for gene expression profiling, and the analysis of differential gene expression in various diseases. Uncover the significance of gene ontology, co-ex

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Understanding Evolutionary Mechanisms: Natural Selection, Genetic Drift, and Gene Flow

Exploring the forces behind evolutionary change, this content delves into natural selection, genetic drift, and gene flow. Examples such as the bottleneck effect, founder effect, and Amish population polydactylism are presented to illustrate how these mechanisms shape genetic diversity. The impact o

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Gene Prediction: Similarity-Based Approaches in Bioinformatics

Gene prediction in bioinformatics involves predicting gene locations in a genome using different approaches like statistical methods and similarity-based approaches. The similarity-based approach uses known genes as a template to predict unknown genes in newly sequenced DNA fragments. This method in

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Understanding Epigenetics: DNA Methylation and Histone Modification

Epigenetics involves modifications that impact gene expression without altering DNA sequences, playing a crucial role in the transition from genotype to phenotype. This includes DNA methylation, histone modification, and microRNAs. DNA methylation, controlled by DNMT enzymes, can lead to either gene

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Genetic Engineering: Techniques, Models, and Applications in Microbial Biotechnology and Plant Genetics

This document explores genetic manipulation, gene manipulation techniques, gene transfer techniques, applications in synthetic and developmental biology, genetically modified organisms/plants, plant tissue culture, micropropagation of plants, genetics in evolution, and genetics in crop improvement m

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Human Insulin Gene Expression and Production

The process of cloning and expressing the human insulin gene has revolutionized the production of insulin for treating diabetes. By using genetically engineered bacteria, the human insulin gene is inserted, expressed, and purified to create insulin for therapeutic use. This innovation has overcome c

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Gene Expression Profiling in Statistical Genetics Summer Institute 2020

This content provides information on the Summer Institutes of Statistical Genetics module, focusing on gene expression profiling. It includes details on the schedule, experimental design, RNA sequencing workflow, modes of bulk RNA sequencing, and RNASeq software. The content discusses crucial steps

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Understanding Gene Mutations in Molecular Biology

Gene mutations play a significant role in molecular biology, leading to alterations in DNA sequences that can impact offspring. These mutations can arise spontaneously or be induced by various factors, such as mutagens. Understanding gene mutations is crucial for comprehending the genetic basis of c

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Genetic Manipulation in Environmental Biotechnology

Genetic manipulation strategies in environmental biotechnology involve techniques like gene splicing and molecular cloning to modify genes directly. These methods have various applications such as isolating genes, producing specific molecules, improving biochemical production, creating organisms wit

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Understanding the Cre-Lox System for Genome Manipulation in Mice

The Cre-Lox system, a powerful tool in genetic engineering, employs Cre recombinase to recombine specific DNA sequences (LoxP sites), allowing for precise genetic modifications such as deletions, inversions, and translocations. This system enables conditional gene expression, turning on or off trans

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Co-Developing Risk Assessments in Gene Drive

Gene drive technology is a cutting-edge approach aimed at combating malaria by spreading genetically modified genes in mosquito populations. This technology has the potential to protect endangered species and conserve nature. However, successful implementation requires complex collaborations, risk a

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Understanding Epigenetics: DNA Methylation and Histone Modification

Epigenetics refers to changes in gene expression without altering the DNA sequence. This involves processes like DNA methylation, histone modification, and microRNAs. DNA methylation is controlled by DNA methyltransferase enzymes and plays crucial roles in gene activation and silencing. Histone modi

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Understanding Ethical Considerations in Manipulated Photography for Media

Ethical control in photojournalism involves capturing non-fiction events without manipulation, but some control over the image is acceptable within ethical bounds. However, moving into manipulated images for non-news events requires a different approach, involving subject and scene manipulation for

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How Advanced Algorithms Are Reshaping Data Manipulation

Explore the transformative impact of advanced algorithms on data manipulation in our latest feature. This insightful exploration delves into the sophisticated technologies revolutionizing business analytics and understanding what is data manipulation

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Basin Manipulation Simulation Analysis Tool Overview

Basin Manipulation Simulation Analysis Tool provides a comprehensive solution for managing and analyzing basin data, including tasks like basin manipulation, simulation analysis, case management, and defining simulation time steps. The tool allows for easy data entry, visualization of simulation res

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Understanding Gene Regulation and Control of Gene Expression

This comprehensive content delves into the intricate mechanisms of gene regulation and control of gene expression. It covers topics such as transcriptional regulation, bacterial genes classification, and the role of regulatory proteins. Explore how genes are regulated at transcription, translation,

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Understanding Gene Expression in Biological Sciences Course

Explore the dynamic world of gene expression with the Chunky and Chips Gene Expression Group at the University of Notre Dame. Dive into topics like transcription, translation, and cellular events influencing gene expression. Delve into real-world scenarios and gain insights into the cellular level b

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Investigating HIF-1 and Apold-1 in Endothelial Permeability at the Blood-Brain Barrier

Understanding endothelial permeability at the blood-brain barrier is crucial for drug delivery to the brain. This research aims to identify a potential mechanism between HIF-1 and Apold-1 specific to cerebral epithelial cells that may impact the integrity of the blood-brain barrier. The study involv

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Analysis of Lady Macbeth's Manipulation in Shakespeare's Macbeth

In a pivotal moment in Macbeth, Lady Macbeth's manipulation of her husband is showcased when she calls upon supernatural forces to overcome his kindness and convince him to kill Duncan. This extract is crucial in introducing Lady Macbeth's character and power dynamics within the play, particularly h

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Understanding Epistasis: Genetic Interactions and Their Implications

Epistasis is a phenomenon where the phenotypic expression of one gene is influenced by interactions with another gene. This concept, first introduced in 1909, plays a crucial role in genetics, affecting various traits and evolutionary processes. The difference between dominance and epistasis lies in

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Gene Wiki Jamboree FaceBase Spring Meeting Summary

The Gene Wiki Jamboree FaceBase Spring Meeting in 2010 aimed to create collaboratively-written and community-reviewed review articles for every gene in the human genome and every concept relevant to FaceBase. It highlighted the value of such articles, providing quick summaries and compilations of re

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Raw Data of Agarose Gel for Human Gene Expression Analysis

This supplementary data includes raw data from agarose gel electrophoresis showing gene expression levels for human SOX17, FOXA2, AFP, ALB, and ACTB in various cellular samples. The gel images depict the expected sizes of amplicons for each gene in different conditions and time points of cell cultur

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Understanding Splice Sites and Splicing Elements in the Inhibitory Dopamine Receptor Gene (D2)

In this project, the focus is on identifying splice sites relevant to the inhibitory dopamine receptor gene (D2). The problem involves finding splicing elements within a specific range of known splice sites in the gene. The data provided includes the D2 gene entry in FASTA format, a database of spli

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Understanding Reverse Genetic Approaches in Gene Manipulation

Reverse genetic approaches involve mutating a target gene to observe its phenotypic effects, providing insights into the gene's normal function. Techniques like gene knockout, knockdown, and homologous recombination are used to manipulate genes and study genetic pathways, leading to discoveries in v

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Basics of Hypothesis Testing in Gene Expression Profiling

The lecture covers the essential aspects of hypothesis testing in gene expression profiling, emphasizing experimental design, confounding factors, normalization of samples, linear modeling, gene-level contrasts, t-tests, ANOVA, and significance assessment techniques. Practical insights are shared on

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Storage and Manipulation of Liquefied Gases with Dewars and Cryostats

Storage and manipulation of liquefied gases involve using specialized equipment like Dewars and Cryostats to minimize heat transfer and maintain low temperatures. Dewars, invented by James Dewar, are double-walled vacuum vessels designed to store liquefied gases with minimal losses. The use of vacuu

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Understanding Direct Manipulation in HCI

In this lecture, learn about the concept of direct manipulation in Human-Computer Interaction (HCI), including its benefits, drawbacks, and related terms such as engagement, distance, semantic, and articulatory distances. Explore the evolution of GUIs and interaction styles, and compare edge maps an

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Basics of Genetics Engineering: Introduction to DNA and Gene Expression

Exploring the fundamentals of genetics engineering, this lecture covers topics such as DNA structure, gene expression, gene function, genome, mutation, recombination, and genetic modification. Understand the significance of DNA, genes, and the processes involved in gene expression. Dive into the wor

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Understanding Expression Vectors for Efficient Gene Expression

Expression vectors are designed to facilitate gene expression by carrying essential elements such as promoters, terminators, and fusion tags. They allow precise control over gene regulation for transcription and translation in host cells, enabling efficient protein production. Different promoters li

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Understanding Narcissistic Abuse: The Insidious Manipulation of Victims

Narcissistic abuse is a form of severe psychological and emotional manipulation that targets a victim's spirit and identity. The abuser employs tactics such as lying, manipulation, denial of reality, and control. This abuse is often invisible and devastating, characterized by a lack of empathy and a

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Cloning the Cstps-1 Gene from Citrus sinensis for Valencene Production

In this project, the Cstps-1 gene from Citrus sinensis is being cloned to produce the enzyme Citrus Valencene Synthase (CVS) responsible for generating the fragrant valencene compound. The process involves DNA isolation from a Valencia orange, gene amplification, vector transformation, and testing f

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Understanding the Role of Multiple Enhancers in Gene Regulation

Multiple enhancers play a crucial role in regulating gene expression in a cell, with approximately 3-5 enhancers associated per human gene. These enhancers are essential for tissue-specific expressions and are active within the same cell line. The use of multiple enhancers can impact gene expression

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Advances in Gene Therapy Programs and Best Practices Workshop Insights

Workshop outcomes and discussions from the MDA-NINDS Workshop on Best Practices for Gene Therapy Programs, highlighting key challenges, possible solutions, and the impact on translational funding programs. The workshop format included orienting talks and panels focusing on scientific premise, regula

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