Gel purification - PowerPoint PPT Presentation


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Water Purification Methods and Techniques Explained

Explore the purification of water on both large and small scales, including storage, filtration, and disinfection processes. Learn about different methods such as boiling, chemical disinfection, and filtration for household water purification. Discover the importance of treatment based on the nature

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mRNA Chromatography Applications: Resin Types and Purification Efficiency

This content delves into mRNA chromatography applications, focusing on the mRNA purification process using dT affinity chromatography, IEX, HIC, and RP techniques. It discusses the application of mRNA affinity resins like Monomix dT20 and Proteomix POR50-dT20, highlighting their properties, purifica

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Gel Electrophoresis DNA Extension Activity in Modern Biology Lab

This lab activity uses agarose gel electrophoresis to determine the length of an unknown DNA fragment by comparing it to known DNA fragment lengths. The process involves gel preparation, loading samples, electrophoresis, staining, de-staining, and measurement of DNA bands. The activity was produced

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Pulsed-Field Gel Electrophoresis: Separating Large DNA Molecules

Pulsed-Field Gel Electrophoresis (PFGE) is a technique developed to effectively separate large DNA molecules through the application of an electric field that periodically changes direction. This method, introduced by David C. Schwartz and Charles C. Cantor in 1984, revolutionized the resolution of

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Separation and Purification of Organic Compounds: Methods and Techniques

The separation and purification of organic compounds are essential processes to obtain pure products, as impurities and side reactions can affect the quality of the compounds. Methods such as solution and filtration, crystallization, distillation, extraction, sublimation, and chromatography are comm

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Understanding Pollution and Purification in Greek Religion

The concept of pollution and purification played a crucial role in maintaining religious and social norms in ancient Greek society. Breaking boundaries or defying societal expectations could lead to pollution, necessitating purification rituals. Examples of actions that could cause pollution include

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Results of SIRT3 Purification from Arctic Express Cells using Urea Wash Protocol

The purification protocol for SIRT3 from Arctic Express cells involved a series of steps utilizing urea washes to remove Cpn60. Two urea wash runs showed effective removal of Cpn60, with greater efficacy observed with 2M urea compared to 1M urea. The gel results indicated successful elimination of C

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AnsCare Silicone Gel with Vitamin C is a top international brand known for reducing hypertrophic scars, keloids, and bump scars caused by injuries or burns. The product contains 100% Medical Grade Silicone Gel with Vitamin C, which helps in minimizing scar discoloration. It is a self-drying, transpa

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Characterization of Cysteine Protease Inhibitor from Canavalia ensiformis

Uncontrolled proteolysis due to cysteine proteases imbalance is crucial in numerous pathological conditions. Canavalia ensiformis, known as the wonder bean, shows potential as a source of cysteine protease inhibitor. The study aims to isolate and characterize the inhibitor from this plant. Methods i

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Getting Started with GEL Scripts in Clarity Educational Community

Explore the power of GEL scripting in Clarity Educational Community with sessions on script exercises, prerequisites, automation benefits, and data sources. Understand what GEL is, its execution language, and how it enables custom logic in CA PPM. Dive into GEL basics, automation advantages, and scr

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Understanding Agarose Gel Electrophoresis in Biochemistry and Molecular Biology

Agarose gel electrophoresis is a technique used to separate and analyze DNA or RNA molecules based on size in biochemistry and molecular biology. It involves applying an electric field to move molecules through an agarose matrix towards the positive electrode, with larger molecules moving slower tha

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Gel Electrophoresis for Assessing Nucleic Acid Quality

Gel electrophoresis is a crucial technique for assessing the quality and yield of nucleic acids such as DNA and RNA. It separates DNA fragments based on size, allowing researchers to determine the integrity of the nucleic acids. By running a small amount of sample on a gel and using DNA-binding dyes

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Understanding Gel Permeation Chromatography (GPC) Technique

Gel Permeation Chromatography is a technique used for separating components based on molecular weight or size. It utilizes columns packed with a porous material to separate molecules by size, allowing smaller molecules to penetrate the gel matrix while larger ones pass through unhindered. Learn abou

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Raw Data of Agarose Gel for Human Gene Expression Analysis

This supplementary data includes raw data from agarose gel electrophoresis showing gene expression levels for human SOX17, FOXA2, AFP, ALB, and ACTB in various cellular samples. The gel images depict the expected sizes of amplicons for each gene in different conditions and time points of cell cultur

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Gel Filtration Chromatography: Separation and Molecular Weight Determination

Gel filtration chromatography is a method used for separating proteins based on their molecular weights. This experiment aims to teach students the principles of gel filtration chromatography and provide hands-on experience in the lab. By utilizing a stationary phase of porous beads and a mobile pha

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Gel Electrophoresis in Genetic Engineering: Principles and Types

Gel electrophoresis is a crucial technique in genetic engineering used to separate macromolecules based on size and charge. Agarose and polyacrylamide gels are the two main types used, each with its advantages and disadvantages. Agarose gel electrophoresis is effective for separating large DNA molec

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Thermal Shock Testing of Gel-Bonded Magnesia Refractories

Study conducted by Paul Bosomworth at Leeds University in 1986 aimed to reduce mechanical damage after thermal shock in magnesia refractories used in sliding gates. The research focused on using gel-binders and achieving optimum grain packing to enhance thermal stress damage resistance. The study al

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Understanding Agarose Gel Electrophoresis: A Comprehensive Overview

Agarose gel electrophoresis is a vital technique used to separate DNA, RNA, or proteins based on their physical properties like size and charge. This seminar covers the definition, working process, and applications of agarose gel electrophoresis, shedding light on its importance in molecular biology

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Understanding Gel Filtration Chromatography: Principles and Applications

Gel filtration chromatography is a molecular size-based separation technique used to separate macromolecules of different sizes. This method, also known as gel permeation or size exclusion chromatography, utilizes a porous stationary phase where small molecules penetrate all pores and are retained,

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Biochemical Methodology 530 - Lactate Dehydrogenase Extraction and Purification Experiment

This course focuses on the extraction and purification of lactate dehydrogenase (LDH) using a series of procedures involving tissue preparation, centrifugation, and ammonium sulfate precipitation. The methodology includes detailed steps from preparing the extraction buffer to isolating LDH in differ

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Protein Purification Techniques and Ammonium Sulfate Precipitation

Protein purification is a crucial process in biotechnology to isolate specific proteins from complex mixtures. Techniques such as exploiting physio-chemical properties, binding affinity tags, and biological activity are used. Ammonium sulfate precipitation, a common first step, involves adding incre

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Understanding DNA Fragment Analysis Through Polyacrylamide Gel Electrophoresis

Polyacrylamide gel electrophoresis is a technique used to separate DNA fragments based on size. By casting a gel and running an electric current through it, terminated DNA fragments can be separated and visualized using isotopes. This process allows for the analysis of DNA fragments differing in siz

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Protein Purification Protocol: Practical Training in Biochemistry Techniques

This protein purification protocol course, led by Dr. Samina Hyder Haq, focuses on practical training in various biochemistry techniques. The course covers organizing experimental protocols, protein isolation strategies, sequential enzyme purification, enzyme kinetics, and writing scientific reports

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Mobile Water Purification System I WOG Group

WOG Group aims to develop one-of-a-kind, long-term water purification and wastewater disposal systems in neighbouring bodies of water. water purification technology that works aids in the preservation of our treasured natural resources. \n\nWebsite:

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Best UV Gel nails in Buttonville

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How to Choose the Right Hydrating Gel Moisturizer

Finding the perfect hydrating gel moisturizer can be challenging, especially with the range of options available today. As skincare needs vary by skin type, climate, and individual preference, selecting a hydrating gel tailored to your needs ensures

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Understanding Distillation in Chemistry: Purification and Separation Methods

Distillation is a crucial process in chemistry for purifying organic compounds by selective evaporation and condensation. It involves the separation of substances based on their boiling points, resulting in either nearly pure components or increased concentrations of specific compounds in a mixture.

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