Agarose gel electrophoresis - PowerPoint PPT Presentation


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Investigating Dehydrogenase Activity Using Yeast

This investigation focuses on exploring respiration in yeast by tracking the breakdown of glucose using dehydrogenase enzymes. The experiment involves immobilizing yeast into gel balls and measuring the change in color of resazurin dye to quantify the rate of respiration. Safety measures, risk asses

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Understanding Plasma Proteins and Their Functions

Plasma proteins play a crucial role in various physiological functions such as controlling extracellular fluid distribution and transportation of hormones, vitamins, and other substances. The balance between protein synthesis and catabolism determines protein levels in the vascular compartment, affe

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Understanding Hydrocollator Packs for Moist Heat Therapy

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Biochemical Reagents Market

Meticulous Research\u00ae\u2014a leading global market research company, published a research report titled, \u2018Biochemical Reagents Market by Type (PCR, Chromatography, Cell & Tissue Culture, Flow Cytometry, Electrophoresis, Immunoassay) End User (Pharmaceutical & Biotechnology, CRO, Diagnostic

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Laboratory Techniques for Biologists: Advanced Biology Unit Overview

Explore essential topics in the field of advanced biology including health and safety protocols, risk assessments, dilution methods, colorimetry, separation techniques like chromatography and electrophoresis, and more. Gain insights on handling hazardous substances, controlling risks, and applying v

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Gel Electrophoresis DNA Extension Activity in Modern Biology Lab

This lab activity uses agarose gel electrophoresis to determine the length of an unknown DNA fragment by comparing it to known DNA fragment lengths. The process involves gel preparation, loading samples, electrophoresis, staining, de-staining, and measurement of DNA bands. The activity was produced

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Pulsed-Field Gel Electrophoresis: Separating Large DNA Molecules

Pulsed-Field Gel Electrophoresis (PFGE) is a technique developed to effectively separate large DNA molecules through the application of an electric field that periodically changes direction. This method, introduced by David C. Schwartz and Charles C. Cantor in 1984, revolutionized the resolution of

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Understanding Blotting Techniques in Animal Biotechnology

Blotting techniques such as Southern blot, Northern blot, and Western blot are used to transfer DNA, RNA, and proteins onto a carrier for separation following gel electrophoresis. Developed by E.M. Southern, the Southern blot specifically detects DNA fragments in a sample through hybridization. This

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Understanding DNA Sequencing: Principles, Applications, and Techniques

DNA sequencing plays a vital role in various fields such as research, diagnostics, biotechnology, forensics, and biological systematics. By determining the order of nucleotide bases in a DNA molecule, it helps in understanding genetic sequences, identifying mutations, and completing projects like th

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PCR and Blot Techniques in Molecular Biology

Polymerase Chain Reaction (PCR) is a crucial technique for amplifying specific DNA segments rapidly and accurately. Developed in 1983, PCR is widely used in various fields such as molecular biology, forensics, evolution, and medical diagnostics. On the other hand, the Blot technique transfers DNA, R

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Electric Properties of Colloids in Liquid Media: Charging Mechanisms and Electrokinetic Phenomena

Colloidal particles dispersed in liquid media can acquire a charge through selective adsorption of ionic species or ionization of surface groups. This leads to electrokinetic phenomena like electrophoresis, electro-osmosis, sedimentation potential, and streaming potential. The stability of colloids

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Understanding Electrophoresis: Principles, Techniques, and Applications

Electrophoresis is a technique dating back to principles of electrochemistry, involving the movement of charged particles in an electric field for separation. Factors affecting electrophoretic mobility include charge, particle size, shape, and applied electrical field. Techniques such as paper, gel,

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Understanding Plasma Proteins in Biochemistry Lectures

This informative content discusses the types, functions, and measurement of plasma proteins in biochemistry. It covers the importance of identifying various plasma proteins, their roles in disease diagnosis, and the interpretation of electrophoretic patterns. The lecture overview provides insights i

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Affinity Chromatography: A Breakthrough in Biochemical Research

Affinity chromatography, developed in the 1930s by A. Wilhelm Tiselius, is a vital technique for studying enzymes and proteins. It relies on the specific affinity between biochemical compounds and utilizes matrices like agarose for binding sites. Ligands such as amino and hydroxyl groups play crucia

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Analysis of Experiment Results: Group 20 Presentation

This presentation showcases the experiment results of Group 20, including findings on food samples, gel electrophoresis data, and implications of potential contamination in plant material and GMO presence. The team consists of Canberk from Turkey, Mehmet Tekinalp, and Elin from Denmark's Thisted Gym

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Understanding Genetic Engineering and Selective Breeding

Genetic engineering involves changing the DNA in living organisms to create new traits, resulting in genetically modified organisms (GMOs). Techniques include artificial selection, cloning, gene splicing, and gel electrophoresis. Selective breeding is a form of artificial selection where animals wit

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Genetic Analysis of Corn Muffin Mix: GMO and Plant-Based Findings

A study conducted on Corn Muffin Mix using electrophoresis revealed that the sample is both genetically modified and plant-based. The results suggest that the GMO used in the study may also be genetically modified. The analysis was supported by visual data and information about the Jiffy brand known

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Laboratory Equipment Maintenance Guidelines for Daily, Weekly, and Monthly Tasks

Regular maintenance tasks for various laboratory equipment including analyzers, flow cytometer, automated gel electrophoresis, and micro hematocrit. The guidelines cover daily, weekly, and monthly checks, cleanings, replacements, and verifications to ensure proper functioning and accurate results. T

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Getting Started with GEL Scripts in Clarity Educational Community

Explore the power of GEL scripting in Clarity Educational Community with sessions on script exercises, prerequisites, automation benefits, and data sources. Understand what GEL is, its execution language, and how it enables custom logic in CA PPM. Dive into GEL basics, automation advantages, and scr

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Understanding Sanger DNA Sequencing Procedure

Overview of Sanger (dideoxy) DNA sequencing, involving deoxynucleotides and dye-labeled dideoxynucleotides to image DNA band sizes. The simulation model includes steps like denaturing DNA, annealing primers, and synthesizing DNA chains using dNTPs and ddNTPs. The process culminates in electrophoresi

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Understanding Agarose Gel Electrophoresis in Biochemistry and Molecular Biology

Agarose gel electrophoresis is a technique used to separate and analyze DNA or RNA molecules based on size in biochemistry and molecular biology. It involves applying an electric field to move molecules through an agarose matrix towards the positive electrode, with larger molecules moving slower tha

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