Effect of Microgravity on Nitrogen Fixation in Red Clover Study

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Investigating the impact of microgravity on nitrogen fixation in red clover at Johnson County Central High School in Tecumseh, NE. The experiment aims to determine if clover exposed to space conditions fixates nitrogen similar to Earth conditions, crucial for growing food in space. Hypothesis, experiment setup, materials used, and initial research findings are presented, along with acknowledgments to sponsors and a call for questions.


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  1. Effect of Microgravity on Nitrogen Fixation in Red Clover (Trifolium pratense L.) Johnson County Central High School, Johnson County, NE

  2. Johnson County Central High School, Tecumseh NE Principal Investigator Rudy Pooch Investigators Isaac Buss Keelee McClintock Natalie Roddy Mason Waring Spencer Dorsey Collaborators Sara Kettelhake Tashayla Dorn Sopha Kongmanyvong Teacher Facilitators Nichole Justesen, Johnson County Central High School Vicky Boone, Johnson County Central High School

  3. Abstract In order to grow our own food in space we need fertile soil. Red clover is a legume that uses bacteria in the soil to naturally fixate nitrogen, making the soil fertile. In this experiment we planted both our sample and control, and will compare how much nitrogen is added to the soil by the clover.

  4. Hypothesis We believe that the clover exposed to microgravity will release the same quantities of nitrates as it would on Earth.

  5. Experiment Our experiment allowed the seeds to germinate in space. The clamps were opened on U-2. The seeds were planted upon return to JCC, and the nitrogen content will be tested throughout its life cycle. Materials: Cotton, Soil (Contains Proteobacteria), Red Clover (Trifolium pratense L), Water

  6. Further Research Week 3 Week 1 Week 2 ISS Control One Control Two Descriptions Week One 12 5 2 Mold in all 3 Week Two 12 1 2 CTL 1 Dry Week Three 9 1 1 Moved outside

  7. National Sponsors State & Local Sponsors JCC School District JCC Board of Education Tecumseh Foundation Barb & Dennis Gottula Ramsey Family Fountain Noman & Ramona Boone Sally Hutt

  8. Questions ?

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