Understanding Microbiology Testing Methods and Results

Explore the various methods of microbiology testing, learn to interpret microbiological test results, and understand antibiotic susceptibility testing. Discover details on bacterial, viral, and parasitic infections through laboratory reports and supporting results like WBC counts and pathogen identification. Delve into bacterial culture, biochemical testing, and antimicrobial susceptibility testing to enhance your knowledge of microbiology standards and identification techniques.

• Microbiology Testing
• Laboratory Reports
• Antibiotic Susceptibility
• Bacterial Culture
• Pathogen Identification

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1. Microbiology Tests What do They Mean? Shawna Dereemer, MT(ASCP) Interim Laboratory Administrator Laboratory Preparedness Supervisor

2. Objectives Summarize various microbiology testing methods List components of a laboratory report Interpret various microbiological test results Summarize antibiotic susceptibility testing and results

3. Wyoming Public Health Laboratory

4. Infectious Disease Bacterial Viral Parasitic

5. Supporting Laboratory Results Viral Identification of virus Normal or Decreased WBC Count Increased Lymphocyte Count Parasitic Identification of parasite Increased Eosinophil Count Bacterial Identification of bacteria Increased WBC Count Increased Neutrophil Count Left-shift in Neutrophil Cell Line Increased CRP Increased Sed-Rate

6. Laboratory Reports

7. Bacterial Culture Grow and isolate bacteria Pros Identify the isolate Very specific Cons Lengthy Normal flora Various media Identify a pathogen

8. Biochemical Testing

9. Identification

10. Microbiology Standards

11. Antimicrobial Susceptibility Testing Determine antibiotic susceptibility or resistance P. aeruginosa Ceftazidime 19mm Intermediate

12. Antimicrobial Susceptibility testing

13. Antimicrobial Susceptibility Testing

14. Antimicrobial Susceptibility Testing P. aeruginosa 0.125 mg/dl Susceptible

15. Antimicrobial Susceptibility testing

16. Antimicrobial Susceptibility Testing

17. Molecular Testing DNA Probes DNA Sequencing Nucleic Acid Sequencing (16s) Whole Genome Sequencing (WGS or NGS) Nucleic Acid Amplification Testing (NAAT) Polymerase Chain Reaction (PCR) Ligase Chain Reaction (LCR) Transcription Mediated Amplification (TMA)

18. PCR

19. DNA Sequencing (16s) Unusual isolates Automated instrumentation will not identify Low confidence levels

20. Whole Genome Sequencing

21. Nucleic Acid Amplification Testing (NAAT) Chlamydia/Gonorrhea testing M. tuberculosis testing

22. Virology PCR No culture isolation

23. Parasitology

24. Immunology/Serology Chlamydia/GC testing HIV Hepatitis B & C

25. Immunology/Serology Quantaferon (IGRA) MMR status (measles, mumps and rubella) Varicella (chicken pox) Syphilis (RPR)

26. Water Lab EPA Water Lab Total Coliform E.coli New environmental testing coming soon

27. Questions

28. Contact Information Shawna Dereemer, MT(ASCP) Interim Laboratory Administrator Laboratory Preparedness Supervisor shawna.Dereemer@wyo.gov 307-777-3735

29. References M100 Performance Standards for Antimicrobial Susceptibility Testing, Clinical and Laboratory Standards Institute, 28th Edition, January 2018.