Overview of Chromatography Techniques and Applications
CHROMATOGRAPHY
Chromatography:
Chromatography is a separation technique used to
separate the different components in a liquid mixture. It
was introduced by a Russian Scientist Michael
Tswett.
Chromatography involves the sample being
dissolved in a particular solvent called mobile phase
.
The mobile phase may be a gas or liquid. The mobile
phase is then passed through another phase called
stationary phase. The stationary phase may be a solid
packed in a glass plate or a piece of chromatography
paper.
The various components of the mixture travel at different
speeds, causing them to separate. There are different types of
chromatographic techniques such as column chromatography,
TLC, paper chromatography, and gas chromatography.
Paper chromatography is one of the important
chromatographic methods. Paper chromatography
uses paper as the stationary phase and a liquid solvent
as the mobile phase. In paper chromatography, the
sample is placed on a spot on the paper and the paper
is carefully dipped into a solvent. The solvent rises up
the paper due to capillary action and the components of
the mixture rise up at different rates and thus are
separated from one another.
Chromatography is a powerful separation technique that involves a mobile phase passing through a stationary phase to separate components in a mixture. Various types of chromatography like column, TLC, paper, and gas chromatography are commonly used for analysis in scientific research and industries. Paper chromatography, a significant method, utilizes paper as the stationary phase and a liquid solvent as the mobile phase for efficient separation of components based on their differential migration rates.
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Presentation Transcript
Chromatography: Chromatography is a separation technique used to separate the different components in a liquid mixture. It was introduced by a Tswett. Chromatography involves the sample being dissolved in a particular solvent called mobile phase. The mobile phase may be a gas or liquid. The mobile phase is then passed through another phase called stationary phase. The stationary phase may be a solid packed in a glass plate or a piece of chromatography paper. Russian Scientist Michael
The various components of the mixture travel at different speeds, causing them to separate. There are different types of chromatographic techniques such as column chromatography, TLC, paper chromatography, and gas chromatography.
Paper chromatography is one of the important chromatographic methods. Paper chromatography uses paper as the stationary phase and a liquid solvent as the mobile phase. In paper chromatography, the sample is placed on a spot on the paper and the paper is carefully dipped into a solvent. The solvent rises up the paper due to capillary action and the components of the mixture rise up at different rates and thus are separated from one another.
