Chromatography for Chemical Analysis
Starter
1.
What are the applications for chemical
analysis?
2.
What is chromatography?
3.
What does the separation of components
depend on?
Learn how differences in size and polarity of
molecules can be used to separate mixtures by
chromatography.
Success Criteria
•
I can use paper chromatography to separate dyes in
two sweets using a variety of solvents (practical)
•
I can breakdown (analysis) why solvents separate
the dyes differently
•
I can decide (evaluate) which solvent is best to
separate the dyes
Learning Intention
Paper
•
http://chemsite.lsrhs.net/FlashMedia/html/p
aperChrom.html
Chromatography experiment
1.
Draw a line with a pencil 2 cm from the bottom of the paper.
2.
Use a paint brush and water to wet your sweet.
3.
Add a small dot of the ink from the paint brush onto the line.
4.
Allow the dot to dry then add another drop of ink. Repeat a
number of times until the spot is concentrated.
5.
Repeat steps 1 to 4 four times so you have four pieces of paper
for both colours
6.
In separate boiling tubes, add 2cm
3
of water, ethanol and
propanone (I have done the toluene for you in the fume hood).
7.
Carefully place the paper into the boiling tubes making sure that
the ink line does not touch the liquid.
8.
Leave the chromatography to run until the solvent is no more
than 3 cm from the end of the paper. Once this happens remove
the paper and draw a line showing where the solvent reached
Things to think about…
•
Is the solvent polar?
•
Which dye moves the furthest?
•
What does that indicate about the
polarity of the dye?
•
What does that indicate about the
polarity of the stationary phase?
•
Why might the dye on the line not
have moved?
Column
•
http://chemsite.lsrhs.net/FlashMedia/html/c
olumnChrom.html
Gas
•
https://www.youtube.com/watch?v=OnQgl
XDvzTc
Explore the applications of chemical analysis, learn about chromatography as a technique for separating components based on size and polarity, conduct paper chromatography experiments, and analyze the results to understand the principles behind this method.
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Presentation Transcript
Starter 1. What are the applications for chemical analysis? 2. What is chromatography? 3. What does the separation of components depend on?
Learning Intention Learn how differences in size and polarity of molecules can be used to separate mixtures by chromatography. Success Criteria I can use paper chromatography to separate dyes in two sweets using a variety of solvents (practical) I can breakdown (analysis) why solvents separate the dyes differently I can decide (evaluate) which solvent is best to separate the dyes
Paper http://chemsite.lsrhs.net/FlashMedia/html/p aperChrom.html
Chromatography experiment 1. 2. 3. 4. Draw a line with a pencil 2 cm from the bottom of the paper. Use a paint brush and water to wet your sweet. Add a small dot of the ink from the paint brush onto the line. Allow the dot to dry then add another drop of ink. Repeat a number of times until the spot is concentrated. Repeat steps 1 to 4 four times so you have four pieces of paper for both colours In separate boiling tubes, add 2cm3 of water, ethanol and propanone (I have done the toluene for you in the fume hood). Carefully place the paper into the boiling tubes making sure that the ink line does not touch the liquid. Leave the chromatography to run until the solvent is no more than 3 cm from the end of the paper. Once this happens remove the paper and draw a line showing where the solvent reached 5. 6. 7. 8.
Things to think about Is the solvent polar? Which dye moves the furthest? What does that indicate about the polarity of the dye? What does that indicate about the polarity of the stationary phase? Why might the dye on the line not have moved?
Column http://chemsite.lsrhs.net/FlashMedia/html/c olumnChrom.html
Gas https://www.youtube.com/watch?v=OnQgl XDvzTc
