Harnessing Phosphate-Solubilizing Microorganisms for Sustainable Crop Growth
Phosphate
solubilizing
Microorganisms
•
Phosphorus
is
one
of
the
major
macronutrient
after
nitrogen
for
growth
and
development
of
a
plant
•
It
is
abundantly
found
in
the
soil
in
the
organic and
inorganic forms
but
it
is
limited
due
to
its
chelation
with another
insoluble
compound.
•
Inorganic
phosphorus
occur
in
soil
mostly
in
insoluble
form
and
therefore
not
available
for
the
plants
nutrition.
•
Inorganic
phosphorus
occur
in
soil
mostly
in
soluble
mineral
complexes
some
of
these
appearing after
the
application
of
chemical
fertilizers.
•
These
precipitated
form cannot
be
absorbed
by
the
plants.
Isolation
of
phosphate
solubilizing
microorganisms
•
Take
one
gram
of
composite
soil
in
1
ml
of
water.
•
Spread
0.1
ml
of
culture
on
nutrient
Agar
media
in
Petri
plates
.
Incubation
should
be
occur
at
37Celsius
for
24
hours.
•
Now
there should
be isolation
of
well
developed
and
separated
colonies
of
pure
culture
bacteria.
•
Study
of
external
morphology
should
be
done
.
•
The
Gram staining
of
bacterial
species
stains
from
pure
culture
slants.
•
Screening
of
bacteria
strain
for
phosphate
solubilizing.
•
Formation
of
halozone
and
bacterial
Colony
on
pkv
Agar
media
confirm
PSP.
•
Now
there
will
be
biochemical
test.
Mass
production
of
PSM
•
The
phosphate
solubilizing
microorganism
stain
in
the
starter
culture
tubes
need
to
be
grown
on
a
large
scale
through
a
mass
production.
•
They
are
transport
to
conical
flask
of
1000
ml
containing
the
appropriate
growth
media and
a
septic
conditions
for
a
small
scale
production
•
1l
of
starter
culture
were
put
into
the
fermented
for
large
scale
production.
•
A
continuous
agitation
and
proper
aeration
for
about
one
week
are
check
time
to
time
for
the
growth
of
cell
mass
.
•
It
should
be
free
from
contamination
.
•
After
one
week
cell
population
in
a
conical
flask
are
stored
into
cooler
temperature
so
that
they
can
be
mixed
properly
with
the
proper
carrier
material..
•
Moreover
it
is
not
advisable
to
keep
conical
for
a
long
time
and
storage
because
of
the
loss
of
the
cell
load.
Application
of
PSMs
in
crop
plants
In
rice
cultivation
•
The
plant
root
development
in
aerobic
rice
was
affected
by
the
application
of
organic
acid
specially
oxalic
acid
PSPBand
PR
.
•
external
application
of
organic
acid
along
with
PSB
and
Insoluble
Phosphorus
in
the
solution
and
this
has
a
positive
impact
on
the
root
growth
.
•
The
bacterial
population
was
influenced
by
the
addition
of
organic
acid
the
oxalic
acid
with
PSB
and PR
increase
the
plant
Biomass.
•
Hormones
IA
that
might
have
an
influence
on
road
growth.
•
The
extensive
root
growth
increase
the
nutrient
update
from
the
surrounding.
•
The
organic
acid
serves
as
the
source
of
carbon
for
the
microorganism
and
effects
the
microbial
population
as well
as
the
plant
growth.
•
With
the
production
of
organic
acid
in
higher
amount
of
phosphorus
in
soil
plant
Phosphorus
update
and
result
plant
higher
Biomass.
•
This
application
is
considered
in
rice cultivation
system
In
wheat
cultivation
•
Bacterial
inoculation
affected
the
early
plant
growth
of
wheat
and
maize.
•
The
bacterial
strains
of
bacillus
and
pseudomonas
has
a
significant
effect
on
the
growth
of
the
wheat
wheat
in
nutrient
poor
soil
by
non
treated
plants
by
comparison
perform
poorly
under
Such
conditions.
•
After
inoculation
bacterial
stain
combine
with
the
phosphoric
and
shoot
length
of
wheat
and
maize
increase
compared
to
inoculated
plant
psm
were
able
to
dissolve
hard
soluble
phosphates
.
•
In
inocculation
also
lead
increase
inN
content
and
increasing
the
growth
and
yield
of
the
Cotton
as
well
as
the
soil
growth
promoting
solublizing
microorganism
please
and
essential
roll
and
helping
the
plants
to
grow
in
the
nutrient
efficient
condition.
Phosphorus is crucial for plant growth, but its availability is often limited due to insoluble forms in soil. The use of phosphate-solubilizing microorganisms (PSMs) can help convert insoluble phosphorus into a usable form for plants. This process involves isolating, screening, and mass-producing PSMs, which can then be applied to crop plants to enhance nutrient uptake and biomass production, as seen in rice cultivation through the application of organic acids along with PSMs and insoluble phosphorus. Utilizing PSMs can lead to improved plant growth and nutrient uptake, contributing to sustainable agriculture practices.
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Presentation Transcript
Phosphate solubilizing Microorganisms
Phosphorus is one of the major macronutrient after nitrogen for growth and development of a plant It is abundantly found in the soil in the organic and inorganic forms but it is limited due to its chelation with another insoluble compound. Inorganic phosphorus occur in soil mostly in insoluble form and therefore not available for the plants nutrition. Inorganic phosphorus occur in soil mostly in soluble mineral complexes some of these appearing after the application of chemical fertilizers. These precipitated form cannot be absorbed by the plants.
Isolation ofphosphatesolubilizing microorganisms Take one gram of composite soil in 1 ml of water. Spread 0.1 ml of culture on nutrient Agar media in Petri plates . Incubation should be occur at 37Celsius for 24 hours. Now there should be isolation of well developed and separated colonies of pure culture bacteria. Study of external morphology should be done . The Gram staining of bacterial species stains from pure culture slants. Screening of bacteria strain for phosphate solubilizing. Formation of halozone and bacterial Colony on pkv Agar media confirm PSP. Now there will be biochemical test.
Mass productionof PSM The phosphate solubilizing microorganism stain in the starter culture tubes need to be grown on a large scale through a mass production. They are transport to conical flask of 1000 ml containing the appropriate growth media and a septic conditions for a small scale production 1l of starter culture were put into the fermented for large scale production. A continuous agitation and proper aeration for about one week are check time to time for the growth of cell mass . It should be free from contamination . After one week cell population in a conical flask are stored into cooler temperature so that they can be mixed properly with the proper carrier material.. Moreover it is not advisable to keep conical for a long time and storage because of the loss of the cell load.
Application of PSMs in crop plants In rice cultivation The plant root development in aerobic rice was affected by the application of organic acid specially oxalic acid PSPBand PR . external application of organic acid along with PSB and Insoluble Phosphorus in the solution and this has a positive impact on the root growth . The bacterial population was influenced by the addition of organic acid the oxalic acid with PSB and PR increase the plant Biomass. Hormones IA that might have an influence on road growth. The extensive root growth increase the nutrient update from the surrounding. The organic acid serves as the source of carbon for the microorganism and effects the microbial population as well as the plant growth. With the production of organic acid in higher amount of phosphorus in soil plant Phosphorus update and result plant higher Biomass. This application is considered in rice cultivation system
In wheat cultivation Bacterial inoculation affected the early plant growth of wheat and maize. The bacterial strains of bacillus and pseudomonas has a significant effect on the growth of the wheat wheat in nutrient poor soil by non treated plants by comparison perform poorly under Such conditions. After inoculation bacterial stain combine with the phosphoric and shoot length of wheat and maize increase compared to inoculated plant psm were able to dissolve hard soluble phosphates . In inocculation also lead increase inN content and increasing the growth and yield of the Cotton as well as the soil growth promoting solublizing microorganism please and essential roll and helping the plants to grow in the nutrient efficient condition.
