Data Analysis and Experimental Results on Cell Activation, Animal Behavior, and Parasite Infection
The analysis covers research findings on cell activation in T cells, mice weight differences, arachnophobia reactions, cane toad parasite infection rates in different areas of Queensland, and neutrophil responses between KO and WT groups. It includes sample size calculations for statistical power in experiments and highlights significant differences observed in the studies.
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Presentation Transcript
Exercises Activated T cells Providing the observed difference between WT and KO cells is of scientific interest, what sample size is needed to achieve a 80% power? Mice weight What sample size is needed to be able to spot a 10% difference with 80% power?
Exercises Arachnophobia Is it as scary to look at the picture of a spider than at a real one? Cane toad Is the proportion of cane toads infected by intestinal parasites the same in 3 different areas of Queensland? Neutrophils Is there a difference between KO with/without treatment and WT?
Activated T cells A ctivated T cells P olarised 40 N ot Polarised 30 C o u n ts 20 10 0 W T K O Answer: To achieve a power of 80%, you will need a total sample of 288 cells.
Mice weight Answer: To be able to spot a 10% difference in the mice weight, at 80% power, you will need a sample n=7.
Arachnophobia 30 20 Difference 10 0 diff -10 -20 Answer: If you are arachnophobe, it is scarier to look at a real spider than at the picture of one (p=0.0310).
Cane toad Answer: The proportion of cane toads infected by intestinal parasites varies significantly between the 3 different areas of Queensland (p=0.0015), the animals being more likely to be parasitized in Rockhampton and Mackay than in Bowen.
Neutrophils Answer: There is a significant difference from WT for the first and third groups.