Understanding DNA Extraction, Quantitation and Amplification
Learn the fundamentals of DNA extraction, including the two categories of extraction, potential issues, and how to determine the extraction category of your sample.
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Understanding Real-Time PCR: A Modern Technique for Nucleic Acid Analysis
Real-time PCR is a powerful tool for amplifying and quantifying DNA or RNA in a sample. It allows for real-time monitoring of the amplification process, enabling precise quantitation of genetic material and gene expression studies. By combining amplification and detection in one step, real-time PCR
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Understanding 10X Single-Cell RNA-Seq Data Analysis
Explore the intricacies of analyzing 10X Single-Cell RNA-Seq data, from how the technology works to using tools like CellRanger, Loupe Cell Browser, and Seurat in R. Learn about the process of generating barcode counts, mapping, filtering, quality control, and quantitation of libraries. Dive into di
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Exploring RNA-Seq Read Mapping and Quantitation
Dive into the world of RNA-Seq expression analysis through topics such as read mapping, quantitation, and the challenges faced in short-read mapping. Discover the typical parameters, design pressures, and basic ideas behind suffix tree index construction for efficient handling of large reads and dat
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RNA-Seq Data Analysis: Mapping Reads to Transcript Abundance
Exploring RNA-Seq data analysis focusing on mapping reads and estimating transcript abundance. Key topics include read mapping speed, quantitation methods, inferences on gene expression, and a simple model for understanding transcript abundance calculation. The process involves linking mapped reads
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Analysis of Moonglow Tomato Phytochemical Composition
This analysis conducted by Jessica Cooperstone at the Schwartz Lab on March 11, 2014, for the Heritage Food Crop Research Trust and Plant & Food Research NZ, delves into the phytochemical composition of Moonglow tomatoes. The study includes detailed data on carotenoid content, such as phytoene, phyt
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Understanding Gas Chromatography: Theory and Applications
Gas chromatography is a widely used technique in various fields such as research, industry, forensics, and environmental analysis. This method allows for the separation and quantitation of compounds based on their different polarities or physical properties. The setup consists of key parts like an i
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SeqMonk Tools for Methylation Analysis Overview
Explore the features and functionalities of SeqMonk for methylation analysis, including data model, raw data visualization, movement controls, quantitation models, probe generation, and targeting measurement methods.
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