Enhancing Agricultural Microbiology Education

The Pandit Jawaharlal Nehru College of Agricultural and Research Institute in Karaikal has been supported by the DBT-STAR college scheme to improve facilities, student exposure visits, introduce new practicals, and enhance faculty development in agricultural microbiology. Key Performance Indicators have been fulfilled, such as introducing new practicals, procuring equipment, executing interdisciplinary projects, and organizing workshops. Students are benefitting from hands-on learning experiences and improved resources, leading to enhanced educational outcomes.

• Agricultural Microbiology
• Education
• Faculty Development

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1. PANDIT JAWAHARLAL NEHRU COLLEGE OF AGRICULTURAL PANDIT JAWAHARLAL NEHRU COLLEGE OF AGRICULTURAL AND RESEARCH INSTITUTE, KARAIKAL 609 603 AND RESEARCH INSTITUTE, KARAIKAL 609 603 Sponsored scheme : DBT-STAR college scheme Total allocated budjet : Agricultural Microbiology Benefited Unit : 3.0 lakhs Coordinator : Dr.P.Pushpakanth, Assistant professor Project coordinator Dr. V. Sundaram, Professor

2. Proposed objectives for the DBT STAR college scheme by Proposed objectives for the DBT STAR college scheme by Agricultural microbiology unit Agricultural microbiology unit 1. To create infrastructural facilities with instruments and chemicals to conduct practical classes for undergraduate students without any hindrance. Accomplished with the due support by DBT 2. To plan for student exposure visit to different institutes which will improve the quality of students and upgrade their knowledge to a greater extent. Accomplished with the due support by DBT

3. The following highlighted Key Performance Indicators fulfilled by The following highlighted Key Performance Indicators fulfilled by Agricultural microbiology unit supported under the scheme Agricultural microbiology unit supported under the scheme a. A. New practicals introduced/planned in Agricultural Microbiology supported under the scheme b. Equipments procured for the proposed new practicals conducted using these equipment c. Interdisciplinary/interdepartmental projected executed/planned by the students d. Workshops and seminars organized/planned for students by the department supported under the scheme e. Faculty improvement programs initiated/planned for the faculty f. Visits to industry and important labs of ntional eminence g. Lectures delivered/planned by experts/speakers in the relevant subject area h. Outreach activities i. Training of lab manpower j Impact of STAR college scheme k. Budget expenditure l. Future activities

4. A. New A. New practicals practicals introduced/planned in Agricultural Microbiology introduced/planned in Agricultural Microbiology supported under the scheme supported under the scheme Earlier there were only 1 microscope for every 5 students. Now, the financial support provided by DBT-SCS facilitate each one with handling individual microscopes.

5. Current I year B.Sc (Hons) Agriculture students involved in Media preparation with newly availed chemicals from DBT scheme Current I year B.Sc (Hons) Agriculture students guided by final year students to separate nodules from legume crop for rhizobium isolation Current I year B.Sc (Hons) Agriculture students invoved and learnt mass multiplication of Azolla in shade net during their practical session New Exopolysaccharide production, consortia development, Blue green algae and azolla mass cultivation) can be effectively taken by the students mainly by availed new chemicals practical experiments (viz, Methylobacterium/Gluconoactobacter isolation,

6. B. Equipments procured for the proposed new B. Equipments procured for the proposed new practicals using these equipment using these equipment practicals conducted conducted A student I year B.Sc (Hons) Agriculture observing specimen from the newly availed microscope by DBT scheme centrifuge Autocalve

7. The following courses gets benefited by DBT The following courses gets benefited by DBT- -SCS fund SCS fund 1. 1. 2. 2. 3. 3. 4. 4. 5. 5. 6. 6. 7. 7. Agricultural microbiology(2+1) Agricultural microbiology(2+1)- - Agriculture Introductory microbiology (1+1) Introductory microbiology (1+1) - - Horticulture Bioinoculant Bioinoculant production technology (0+10) production technology (0+10) Applied microbiology (2+1) Applied microbiology (2+1) - - Agriculture Environmental studies and disaster management (2+1) Environmental studies and disaster management (2+1) - -Agriculture Environmental studies and disaster management (1+1) Environmental studies and disaster management (1+1) - -Horticulture Basic laboratory skills (0+2) Basic laboratory skills (0+2) Post graduate students Post graduate students Agriculture Horticulture Agriculture Agriculture Horticulture With the financial support by DBT-SCS, glasswares, chemicals, intruments/equipments and stationaries is now suffficient to carryout all the practical experiments without any hindrance by the students.

8. C. Interdisciplinary/interdepartmental projected executed/planned C. Interdisciplinary/interdepartmental projected executed/planned by the students by the students Course Course Bioinoculant (0+10) Duration Duration 5 months Target Target Publication in NAAS rated journal/Conference/symposium/product development/skill development. The following 5 different areas were chosen for each group which consist of 6 students. Each of th 5 group has been alloted with Methylobacterium, Azospirillum, ZSB and Azotobacter. code code and and production title title ELP414 technology rhizobium,

9. ISOLATION, CHARACTERIZATION AND SCREEENING ISOLATION, CHARACTERIZATION AND SCREEENING OF RHIZOBIUM FROM NODULES OF DIFFERENT OF RHIZOBIUM FROM NODULES OF DIFFERENT LEGUME CROPS AND THEIR GROWTH PROMOTING LEGUME CROPS AND THEIR GROWTH PROMOTING EFFECT ON BLACK GRAM ( EFFECT ON BLACK GRAM (Vigna mungo Vigna mungo) ) Team members: Anbuvanan. P ArokiaSheela. A Dharshinii. PA Eswar Chandra Vidyasagar. Y Sharan. R Siranjeevi. R

10. Objectives Objectives 1. Isolation of Rhizobium from root nodule of different pulse crops. 2. Characterization of refined isolates for confirmation of Rhizobium by authentication tests. 3. To evaluate the Rhizobium for their nodulation efficiency by seed inoculation assay

11. Details of sa Details of sam mple collection and isolate details ple collection and isolate details Crop name Crop name Variety Variety Age at the time Age at the time Previous Previous Soil type Soil type Place of collection Place of collection Isolates in hand Isolates in hand of collection of collection crop crop Lab Lab CO 4 45 DAS - Sandy loam Karaikal 13 Blackgram VBN 8 70 DAS - - Dindigul 24 Groundnut TMV 7 70 DAS Blackgram Clay loam soil Nettapakkam (Pondicherry) 4 Groundnut TMV 7 55 DAS Paddy Red soil Alanganallur (Madurai) 8 Blackgram ADT 6 45 DAS Paddy Clay loam soil Karukangudi (Karaikal) 5 Groundnut G 7 90 DAS Groundnut Sandy soil Thirukadaiyur 7 Groundnut TMV 7 81 DAS Bhendi Sandy loam Cuddalore 10 Sesbania - 20 DAS - - Karaikal 5 Groundnut - 90 DAS Groundnut Red soil Karaikudi 6 Groundnut G7 90 DAS Brinjal Sandy loam Cuddalore 4 Total Total 86 86 Rhizobiu Rhizobium strains m strains

12. Rhizobium team activities

13. ISOLATION,CHARACTERIZATION AND SCREENING OF EPIPHYTIC ISOLATION,CHARACTERIZATION AND SCREENING OF EPIPHYTIC PINK PIGMENTED FACULTATIVE METHYLOTROPHS FROM PINK PIGMENTED FACULTATIVE METHYLOTROPHS FROM DIFFERENT HOST PLANTS AND THEIR GROWTH PROMOTING DIFFERENT HOST PLANTS AND THEIR GROWTH PROMOTING EFFECT ON RICE ( EFFECT ON RICE ( Oryzae Oryzae Sativa ) Sativa ) Team members: Team members: 1. AGILA.P AGILA.P 2. GOKULAKRISHNAN.B 2. GOKULAKRISHNAN.B 3. MALINI.K 3. MALINI.K 4. PRASANNA.A 4. PRASANNA.A 5. SARUMATHY.S 5. SARUMATHY.S 6. VIDHYA.K 6. VIDHYA.K

14. Objectives Objectives 1. Isolation of Methylobacterium from Philosphere of different host crops. 2. Characterization of refined isolates for confirmation of Methylobacterium 3. Screening of selected Methylobacterium for their plant growth promoting traits 4. To evaluate the Methylobacterium for their PGP trait by seed inoculation assay

15. Details of sample collection and the isolate details HOST CROP HOST CROP AGE AGE PREVIOUS PREVIOUS CROP CROP PLACE OF PLACE OF COLLECTION COLLECTION NO OF NO OF COLONY IN COLONY IN Abaxial Abaxial NO OF NO OF COLONY COLONY IN IN Adaxial Adaxial NO OF NO OF ISOLATES ISOLATES NO OF NO OF STRAIN STRAIN IN HAND IN HAND BEAN 50 DAYS BEAN PAJANCOA &RI 69 5 2 MB1 ,MB2 SUNFLOWER 90 DAYS GROUNDNUT PAJANCOA &RI 2 2 2 MB 3,MB4 TOMATO 56 DAYS BHENDI PAJANCOA &RI 49 33 2 MB 5,MB6 GROUNDNUT (TMV 7) 70 DAYS PADDY NETTAPAKKAM 2 _ 2 MB7, MB8 BEAN 65 DAYS YARD LONG BEAN PAJANCOA&RI 37 22 2 MB9 MB10, EUCALPYTUS 10 YEARS - PAJANCOA&RI 11 6 2 ,MB11, M B12 TOMATO 45 DAYS - SATHANOOR 2 3 2 MB13,MB 14 MULBERRY MORE THAN 4 YEARS MULBERRY PAJANCOA &RI 1 2 2 MB15,MB 16 GROUNDNUT 90 days GROUNDNUT AMARAVATHIP UTHUR 2 2 2 MB17,MB 18

16. Student activities Student activities The seeds are sown in test tubes containing water agar media Sterilization of paddy seeds

17. Student activities Student activities

18. Product launched through ELP Product launched through ELP- -414 course 414 course

19. D. Workshops and seminars organized/planned for students by D. Workshops and seminars organized/planned for students by the department supported under the scheme the department supported under the scheme Among (Methylobacterium, Rhizobium and Zinc solubilizing bacteria) were attended for National conference held at SBACRF, karaikudi. FUNDED BY DBT-SCS the 5 groups, 3 group

20. BEST POSTER AWARD BEST POSTER AWARD Gokulakrishnan Gokulakrishnan, , B B. ., , Malini K, K, Sarumathy Sarumathy, , S, S, Jananee Sundaravarathan Sundaravarathan, , Pouchepparadjou Pouchepparadjou, , A A. . 2023 on on Enhancing Enhancing Agriculture Agriculture Agricultural Agricultural college college and karaikudi karaikudi. . July July- -22 Malini, , K, Jananee, , S, S, Pushpakanth S, S, Ramesh Ramesh, , 2023. . National National conference sustainability sustainability and and resilience (ESRA (ESRA- -2023 2023) ). . and Research Research foundation, 22. . K, Agila Agila, , P, P, Vidhiya Pushpakanth, , P*, Vidhiya, , P*, and and V V conference resilience of of SethuBhaskara SethuBhaskara foundation, 20

21. F. Visits to industry &important labs of national eminence F. Visits to industry &important labs of national eminence EXPOSURE VISIT UNDER STAR COLLEGE SCHEME (DBT) EXPOSURE VISIT UNDER STAR COLLEGE SCHEME (DBT) Course Course code code & & title title EXP424 Bioinoculant production technology (0+5) Date Date of of visit visit 11.07.2022 to 14.07.2022 (4 days) Organizing Organizing department department - Agricultural microbiology unit, PAJANCOA & RI Place Place of of visit visit- Deprtment of Agricultural Microbiology, TamilNadu Agricultural University, Coimbatore. Accompanied Accompanied staff staff Dr. A. Premkumar Dr. M.Manikandan No No of of students students engaged engaged- 37 (18 boys and 19 girls) Outcome Outcome : The students learnt production of different biofertilizers because of this exposure visit along with culture preservation, carrier preparation, dos/don t, production, annual turnover and marketing scenario etc The students gained greater exposure, knowledge and skills rather than taking classes in classrooms. It paves way for the students to improve their enterpreneurship skills As per the objective, an exposure visit As per the objective, an exposure visit has been arranged to learn about the has been arranged to learn about the biofertilizer biofertilizer production and processing. production and processing. about the large scale

22. Budget Budget Amount Amount alloted alloted (in lakhs lakhs) ) Amount spent Amount spent (in 000`) (in 000`) Balance Balance K. Budget K. Budget expenditure expenditure (Rs. 21,137 utilized by pathology thro mutual understanding) (in Non-Recurring 3.0 3.0 Lakhs Lakhs 2,78,863.00 Nil Recurring 0.75 0.75 lakhs lakhs Nil a. Chemicals 33660.00 b. Glasswares 20438.00 c. Exposure visit 20902.00 Contigency 0.10 0.10 lakhs lakhs 10000.00 Nil Total Total 3.85 3.85 lakhs lakhs 3,63,863.00 3,63,863.00 Nil Nil Remark / Payment Voucher No. with Date Proposed Unit Cost (Rs.) Number ofUnits Actual Cost (000) Date of PlacingOrder Date of Receiving Equipment instrument/equipment 781/A4 dated 20.03.2023 Microscope Micron optic KG-5 9735.00 17 1,65,495.00 31.01.2023 08.03.2023 781/A4 dated 20.03.2023 SANDY Vertical Autoclave 62068.00 01 62068.00 08.03.2023 31.01.2023 782/A4 dated 20.03.2023 782/A4 dated 20.03.2023 REMI R8C PLUS centrifuge 42704.00 01 42704.00 31.01.2023 18.02.2023 Centrifuge Rotor 8596.00 01 8596.00 24.02.2023 06.03.2023 Total Total 2,78,863.00

23. L. Future activities L. Future activities The effective bio inoculants identified with the assistance from DBT SCS need to be evaluated under both pot culture and field level experiments to market the biofertilizer for the benefitof farmers. 1. For capacities can adopt for mass multiplication of desired products (carrier and liquid based formulations) in a faster manner. large scale production, fermentors with larger 2. Moreover students can successfully involved in this mass production activity may development rather than taking students for bioferilizer production units. leads to enterpreunership 3.