State-of-the-Art Facilities at Central Instrumental Facility, Bihar Animal Sciences University, Patna

The Central Instrumental Facility at Bihar Animal Sciences University in Patna offers a range of cutting-edge equipment for research purposes, including an electroporator, bench-top flow cytometer, real-time PCR machine, inverted fluorescence microscope, and more. Researchers can utilize these facilities for various applications such as immunophenotyping, apoptosis and necrosis studies, gene expression analysis, cell culture studies, and more. Additionally, the facility provides tools for DNA/RNA concentration and purity assessment. Explore the advanced capabilities of this facility to enhance your research endeavors.

• Research Facilities
• Bihar Animal Sciences University
• Instrumental Equipment
• Biotechnology
• Patna

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1. FACILITIES AVAILABLE AT CENTRAL INSTRUMENTAL FACILITY At Bihar Animal Sciences University Patna

2. S. No. Name of equipment S. No. Name of equipment Electroporator 1. Bench Top Flowcytometer 9. Semi dry blot apparatus 2. Real Time PCR 10. Inverted Fluorescence attachment Nano spectrophotometer Microscope with Analytical Balance Weighing 11. 3. Photograph 12. Precision balance 4. High centrifuge speed refrigerated micro 13. 5. Digital Vortex mixture Refrigerated circulator and heating bath 14. 6. Portable pH meter 7. -80 C Upright ultra low deep 15. Microcentrifuge freezer -20 C Upright Freezer 16. 8. Binocular Microscope

3. Bench Top Flowcytometer

4. APPLICATION Blue and red filter FITC,PE,APC,GFP, ALEXA Immunophenotyping-Florescence tag antibody/Kit Apoptosis- Kit- Beckman Coulter/ B.D Biosciences Necrosis study- Propidium Iodide (PI)- Sigma Cell cycle and DNA ploidy- Propidium Iodide (PI)- Sigma Transfection study- GFP Stem cell enumeration- Florescence tag antibody Mitochondrial study- JC-1- Sigma ROS study- DCFDA- Sigma

5. Real time PCR

6. APPLICATION Gene Expression analysis SYBR Green/EVA green- Fermatas/ Agilent/Invitrogen Florescence tag Probe- Xceleris Diagnosis of Infectious Diseases Florescence tag Probe- Xceleris

7. Inverted Microscope

8. APPLICATION Transfection study-Florescence tag antibody Immunohistochemistry-Florescence tag antibody Cell culture Study

9. Nano Spectrophotometer

10. APPLICATION Concertation of DNA/RNA Purity of DNA/RNA

11. Refrigerated Centrifuge

12. APPLICATION 1.5 ml rotor 15 ml rotor 50 ml rotor Speed 17000rpm DNAI solation- Kit- Fermatas/ Invitrogen RNA Isolation- Trizol- Sigma Kit- Fermatas/ Invitrogen

13. Refrigerated and heating water bath

14. APPLICATION Used between 4 to 100 degree C Transformation Ligation reaction- Ligase- Fermatas/ NEB Restriction digestion- Restriction enzyme- Fermatas/ NEB

15. -80C Upright ultra low deep freezer

16. APPLICATION Long term Storage of sample

17. -20C Upright Freezer

18. APPLICATION Storage of sample Storage of Reagent /Kit

19. Electroporator

20. APPLICATION High efficacy Transformation Bacterial genome editing Cuvette- Bio-Rad

21. Semi dry blot apparatus

22. APPLICATION Western Blotting

23. Precision balance Analytical Balance

24. Portable pH meter Vortex

25. Binocular Microscope

26. Serum Repository Department of Biochemistry mentioning the serum repository obtained from VCC Planning of research

27. Practical Utility of Equipment DNA/ RNA Isolation Cloning Expression of protein SDS PAGE Western Blotting FACS Analysis Expression analysis by real time PCR Immunohistochemistry Bacterial genome editing Storage of samples