Gene Expression in Biological Sciences Course
“Chunky and Chips”
Gene Expression Group
University of Notre Dame
Kristin Hager, Associate Teaching Professor,
Biological Sciences
Joseph O’Tousa, Professor, Biological Sciences
WH Freeman
Marc Mazzoni, Senior Editor, Life Sciences
Elaine Palucki, Editor, Adjunct Assistant
Professor Biology, Brooklyn College
Susan Winslow, Executive Editor, Life Sciences
Set- Up:
T
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:
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:
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:
These topics have been completed:
Central Dogma, transcription, translation.
Ready for a synthesis of what they have already learned
By the end of lecture today you
should be able to:
L
e
a
r
n
i
n
g
G
o
a
l
:
U
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Learning Outcomes:
•
Describe an everyday activity that alters gene expression.
•
Describe the cellular location for the events of gene
expression
•
Draw and interpret multiple types of graphs to display gene
expression
Case: Elaine is working out, Marc is
more sedentary likes to eat
chocolate chip cookies…
Kristin’s undergrads want to do a
muscle biopsy to see if anything
different is happening on the cellular
level
Skeletal Muscle
muscle cell that
contains alpha-myosin
Picture of myosin protein here…
M
y
o
s
i
n
B
a
c
k
g
r
o
u
n
d
I
n
f
o
r
m
a
t
i
o
n
:
Which of the cells in the
tissues shown have a
gene
for
myosin?
A.
Muscle cells
B.
All cells
C.
Skin and
muscle cells
D.
Sperm cell
Liver
Sperm
Which of the cells in the
tissues express the
protein
for
myosin?
A.
Muscle cells
B.
All cells
C.
Skin and
muscle cells
D.
Sperm cell
Sperm
Liver
Definition of Gene Expression
of
-myosin
T
r
a
n
s
c
r
i
p
t
i
o
n
T
r
a
n
s
l
a
t
i
o
n
D
N
A
R
N
A
P
r
o
t
e
i
n
(
a
l
p
h
a
-
m
y
o
s
i
n
)
Complete this picture of a cell
undergoing gene expression using the list
of molecules and processes given.
nucleus
M
o
l
e
c
u
l
e
s
a
n
d
P
r
o
c
e
s
s
e
s
Transcription
Translation
DNA
mRNA
Protein – alpha-myosin
Finish the bar graph that shows the levels of
each molecule in Marc and Elaine’s muscle cells
following a week of activity:
A
m
t
p
e
r
c
e
l
l
D
N
A
R
N
A
P
r
o
t
e
i
n
(
m
y
o
s
i
n
)
Marc
Finish the bar graph that shows the levels of
each molecule in Marc and Elaine’s muscle cells
following a week of activity:
A
m
t
p
e
r
c
e
l
l
D
N
A
R
N
A
P
r
o
t
e
i
n
Marc
Marc
Marc
Elaine
Elaine
Elaine
Now add Elaine’s liver cell levels of each:
A
m
t
p
e
r
c
e
l
l
D
N
A
R
N
A
P
r
o
t
e
i
n
Marc
Marc
Marc
Elaine
Elaine
Elaine
Elaine’s
Liver
?
Elaine’s
Liver
?
Elaine’s
Liver
?
Now add Elaine’s liver cell levels of each:
A
m
t
p
e
r
c
e
l
l
D
N
A
R
N
A
P
r
o
t
e
i
n
Marc
Marc
Marc
Elaine
Elaine
Elaine
Elaine’s
Liver
Elaine’s
Liver
Elaine’s
Liver
Create three line graphs that shows how each molecule
might change over time in the muscle. First show
Elaine:
T
i
m
e
(
d
a
y
s
)
D
N
A
m
R
N
A
P
r
o
t
e
i
n
|
|
|
|
|
|
|
1
2
3
4
5
6
7
Now a line indicating Marc’s expression levels
T
i
m
e
D
N
A
m
R
N
A
P
r
o
t
e
i
n
Elaine
Elaine
Elaine
Now a line indicating Marc’s expression levels
T
i
m
e
D
N
A
m
R
N
A
P
r
o
t
e
i
n
Elaine
Elaine
Elaine & Marc
Marc
Marc
Gene Expression is like this picture:
Liver
Marc Elaine
What part of this diagram represents
the DNA?
Liver
Marc Elaine
A)
T
h
e
f
a
u
c
e
t
B)
T
h
e
w
a
t
e
r
C)
I
’
m
n
o
t
s
u
r
e
…
Now that it’s the end of lecture
you have shown that you….
1.
U
n
d
e
r
s
t
a
n
d
t
h
a
t
g
e
n
e
e
x
p
r
e
s
s
i
o
n
i
s
d
y
n
a
m
i
c
(
t
e
m
p
o
r
a
l
,
l
e
v
e
l
o
f
m
R
N
A
,
p
r
o
t
e
i
n
p
r
o
d
u
c
e
d
)
•
Described an everyday activity that alters gene
expression.
•
Described the cellular location for the events of
gene expression
•
Worked with multiple types of graphs to display
gene expression
For homework, think about:
1.
In our cells, what will act
as the handle on the
faucet?
2.
How might our cells
control whether the
handle is turned on or
off?
3. How might our cells
control how much water
(mRNA) is being
released from the
faucet?
Definition of a gene promoter
•
The handle of the spigot
•
(include real definition here and a diagram
with coding information and RNA being made
as an arrow away from the promoter)
Which cells will express the myosin
gene?
•
What do you know?
•
What do you need to
know?
INDUCTION OF SLOW MYOSIN
GENE IN FAST-TWITCH MUSCLE
Am. J. Physiol. Cell Physiol- 1996 Barton Davis
How do you know that the myosin
gene is being expressed?
•
You have 30 seconds to talk to your neighbor
Explore the dynamic world of gene expression with the Chunky and Chips Gene Expression Group at the University of Notre Dame. Dive into topics like transcription, translation, and cellular events influencing gene expression. Delve into real-world scenarios and gain insights into the cellular level by examining myosin gene expression in different tissues.
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Presentation Transcript
Chunky and Chips Gene Expression Group University of Notre Dame Kristin Hager, Associate Teaching Professor, Biological Sciences Joseph O Tousa, Professor, Biological Sciences WH Freeman Marc Mazzoni, Senior Editor, Life Sciences Elaine Palucki, Editor, Adjunct Assistant Professor Biology, Brooklyn College Susan Winslow, Executive Editor, Life Sciences
Set- Up: Topic: Gene Expression Course: Introductory Majors Biology (First university level biology course) Course Name: Introductory Biology, week 4 of semester Syllabus Placement: These topics have been completed: Central Dogma, transcription, translation. Ready for a synthesis of what they have already learned
By the end of lecture today you should be able to: Learning Goal: Understand that gene expression is dynamic Learning Outcomes: Describe an everyday activity that alters gene expression. Describe the cellular location for the events of gene expression Draw and interpret multiple types of graphs to display gene expression
Case: Elaine is working out, Marc is more sedentary likes to eat chocolate chip cookies Kristin s undergrads want to do a muscle biopsy to see if anything different is happening on the cellular level
Background Information: Skeletal Muscle muscle cell that contains alpha-myosin Picture of myosin protein here Myosin
Which of the cells in the tissues shown have a gene for myosin? A. Muscle cells B. All cells C. Skin and muscle cells D. Sperm cell Liver Sperm
Which of the cells in the tissues express the protein for myosin? A. Muscle cells B. All cells C. Skin and muscle cells D. Sperm cell Liver Sperm
Definition of Gene Expression of -myosin DNA Transcription RNA Translation Protein (alpha-myosin)
Complete this picture of a cell undergoing gene expression using the list of molecules and processes given. Molecules and Processes Transcription Translation DNA mRNA Protein alpha-myosin nucleus
Finish the bar graph that shows the levels of each molecule in Marc and Elaine s muscle cells following a week of activity: Amt per cell Marc DNA RNA Protein (myosin)
Finish the bar graph that shows the levels of each molecule in Marc and Elaine s muscle cells following a week of activity: Elaine Elaine Amt per cell Marc Marc Marc Elaine DNA RNA Protein
Now add Elaines liver cell levels of each: Elaine Elaine Amt per cell Elaine s Liver Elaine s Liver Elaine s Liver Marc Marc Marc Elaine ? ? ? DNA RNA Protein
Now add Elaines liver cell levels of each: Elaine Elaine Amt per cell Marc Marc Elaine s Liver Marc Elaine Elaine s Liver Elaine s Liver DNA RNA Protein
Create three line graphs that shows how each molecule might change over time in the muscle. First show Elaine: Protein mRNA DNA | 1 | 2 | 3 | 4 | 5 | 6 | 7 Time (days)
Now a line indicating Marcs expression levels Elaine Protein Elaine mRNA DNA Elaine Time
Now a line indicating Marcs expression levels Elaine Protein Marc Elaine mRNA Marc DNA Elaine & Marc Time
Gene Expression is like this picture: Liver Marc Elaine
What part of this diagram represents the DNA? Liver Marc Elaine A)The faucet B)The water C)I m not sure
Now that its the end of lecture you have shown that you . 1. Understand that gene expression is dynamic (temporal, level of mRNA, protein produced) Described an everyday activity that alters gene expression. Described the cellular location for the events of gene expression Worked with multiple types of graphs to display gene expression
For homework, think about: 1. In our cells, what will act as the handle on the faucet? 2. How might our cells control whether the handle is turned on or off? 3. How might our cells control how much water (mRNA) is being released from the faucet?
