Gas Chromatography Techniques and Applications
•
Since the partitioning behavior is depend
e
nt on
temper
a
ture, the
separation column is usually
contained in a thermostat-controlled oven.
•
Separating components
with a wide range of
boiling points is accomplished by starting at a low
oven temperature and
increasing the temperature
over time to elute the high-boiling point
components.
A gas chromatography oven, open
to show a capillary
column
•
The mobile phase (carrier gas) should be chemically inert,
dry, and free from O
2
(helium, argon, nitrogen and hydrogen).
•
The carrier gas should be of high purity; impurities in the
carrier gas such as water vapour, air and trace gaseous
hydrocarbons can cause reactions with sample and cause
column deterioration and affect detector performance.
•
The gas supply is associated with pressure regulator and
flow controller.
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Samples GC must be volatile.
Samples which are non volatile are converted into a volatile derivative.
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Most GC columns are made from high-purity fused silica capillary, the inner wall of
the capillary coated with the stationary phase.
GC columns vary in length from less than 2 m to 50 m or more.
In order to fit into the column oven, they are usually formed as coils.
The control of column’s temperature is critical to attain a good separation in GC,
thus the column is located inside a thermostated oven to control the temperature.
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Thermal conductivity detector (TCD)
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Flame ionization detector (FID)
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Nitrogen phosphorous detectors (NPD)
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Food Analysis
Analysis of foods is concerned with confirming the presence
and determination the quantities of the analytes (lipids,
proteins, carbohydrates, preservatives, flavors, colorants,
and also vitamins, steroids, and pesticide residues).
•
Drug Analysis
GC is widely applied to identification of the active components,
possible impurities as well as the metabolites.
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Forensic Analysis
In forensic cases, very little sample is available, and the
concentration
of
the sample components may be very low.
GC is a useful due to its high sensitivity and separation
efficiency.
•
Environmental Analysis
The environmental contaminants; e.g. (DDT) is present in the
environment at very low concentrations and are found among
many of other compounds.
GC, with its high sensitivity and
high separating power, is mostly used in the analysis of
environmental samples.
Comparison of HPLC and GC
Sample Volatility
Sample Polarity
HPLC
•
No volatility requirement
•
Sample must be soluble
in mobile phase
GC
•
Sample must be volatile
HPLC
GC
•
Separates both polar and
non polar compounds
•
Samples are nonpolar
and polar
Comparison of HPLC and GC
Sample Preparation
Sample Size
HPLC
•
Sample must be filtered
•
Sample should be in
same solvent as mobile
phase
GC
•
Solvent must be volatile
and generally lower
boiling than analytes
HPLC
GC
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Sample size based upon
column.
•
Typically 1 - 5
L
Comparison of HPLC and GC
Gas chromatography is a powerful analytical technique used for separating and analyzing volatile compounds in various industries such as food analysis and drug identification. The process involves a separation column contained in a thermostat-controlled oven, along with a carrier gas system and detectors for accurate analysis. By controlling temperatures and elution times, GC provides precise separation of components with different boiling points, making it an essential tool for research and quality control.
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Presentation Transcript
Since the partitioning behavior is dependent on temperature, the separation column is usually contained in a thermostat-controlled oven. Separating components with a wide range of boiling points is accomplished by starting at a low oven temperature and increasing the temperature over time to elute the high-boiling point components.
http://upload.wikimedia.org/wikipedia/commons/thumb/7/74/GC_Oven_inside.jpg/300px-GC_Oven_inside.jpghttp://upload.wikimedia.org/wikipedia/commons/thumb/7/74/GC_Oven_inside.jpg/300px-GC_Oven_inside.jpg A gas chromatography oven, open to show a capillary column
Pressure regulator Flow controller Gas Supply Gas Supply Gas supply The mobile phase (carrier gas) should be chemically inert, dry, and free from O2 (helium, argon, nitrogen and hydrogen). The carrier gas should be of high purity; impurities in the carrier gas such as water vapour, air and trace gaseous hydrocarbons can cause reactions with sample and cause column deterioration and affect detector performance. The gas supply is associated with pressure regulator and flow controller.
Flow meter Flow controller Septum Detector GC chart Injector Pressure regulator Recorder Sample Injection System Sample Injection System Oven Column Gas supply Preparation of the Sample Preparation of the Sample : : Samples GC must be volatile. Samples which are non volatile are converted into a volatile derivative. GC Column GC Column Most GC columns are made from high-purity fused silica capillary, the inner wall of the capillary coated with the stationary phase. GC columns vary in length from less than 2 m to 50 m or more. In order to fit into the column oven, they are usually formed as coils. The control of column s temperature is critical to attain a good separation in GC, thus the column is located inside a thermostated oven to control the temperature.
GC Detectors GC Detectors Thermal conductivity detector (TCD) Flame ionization detector (FID) Nitrogen phosphorous detectors (NPD)
GC Applications GC Applications: : Food Analysis Analysis of foods is concerned with confirming the presence and determination the quantities of the analytes (lipids, proteins, carbohydrates, preservatives, flavors, colorants, and also vitamins, steroids, and pesticide residues). Drug Analysis GC is widely applied to identification of the active components, possible impurities as well as the metabolites.
Environmental Analysis The environmental contaminants; e.g. (DDT) is present in the environment at very low concentrations and are found among many of other compounds. GC, with its high sensitivity and high separating power, is mostly used in the analysis of environmental samples. Forensic Analysis In forensic cases, very little sample is available, and the concentration of the sample components may be very low. GC is a useful due to its high sensitivity and separation efficiency.
Comparison of HPLC and GC Sample Volatility Sample Polarity HPLC No volatility requirement HPLC Separates both polar and non polar compounds Sample must be soluble in mobile phase GC Samples are nonpolar and polar GC Sample must be volatile
Comparison of HPLC and GC Sample Preparation Sample Size HPLC Sample must be filtered HPLC Sample size based upon column. Sample should be in same solvent as mobile phase GC GC Typically 1 - 5 L Solvent must be volatile and generally lower boiling than analytes
Comparison of HPLC and GC Separation Mechanism Detectors HPLC Both stationary phase and mobile phase take part HPLC Most common UV-Vis Wide range of non- destructive detectors 3-dimensional detectors GC GC Most common FID, universal to organic compounds Mobile phase is a sample carrier only
