Enzyme Reaction Assay with 4NPbutyrate and Acetonitrile
Conducted enzyme reaction assays using 4NPbutyrate in the presence of acetonitrile and other compounds. Varying concentrations of 4NPbutyrate were tested over time, and reaction rates were analyzed. Results were recorded for different reaction conditions, providing valuable insights into enzyme kinetics.
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S72A 4NPbutyrate 1/50 Acetonitrile 5% tritonx 0.5% buffer plate assay [enzyme] in reaction = 0.37 M 0.0085 0.0035 0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 Time (secs) Vmax Points = 26 Well Vmax R^2 B1 1.474 0.993 0.0104 0.0054 0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 Time (secs) Vmax Points = 26 Well Vmax R^2 B2 1.576 0.993 0.0099 0.0049 0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 Time (secs) Vmax Points = 26 Well Vmax R^2 B3 1.655 0.982 [4NPbutyrate] 10 M
0.062 0.042 0.022 0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 Time (secs) Vmax Points = 26 Well Vmax R^2 C1 8.944 0.996 0.064 0.044 0.024 0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 Time (secs) Vmax Points = 26 Well Vmax R^2 C2 9.060 0.997 0.062 0.042 0.022 0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 Time (secs) Vmax Points = 26 Well Vmax R^2 C3 8.395 0.996 [4NPbutyrate] 50 M
0.148 0.098 0.048 0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 Time (secs) Vmax Points = 26 Well Vmax R^2 D1 19.774 0.991 0.149 0.099 0.049 0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 Time (secs) Vmax Points = 26 Well Vmax R^2 D2 20.017 0.992 0.096 0.046 0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 Time (secs) Vmax Points = 26 Well Vmax R^2 D3 19.430 0.993 [4NPbutyrate] 100 M
0.41 0.31 0.21 0.11 0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 Time (secs) Vmax Points = 26 Well Vmax R^2 E1 64.802 0.985 0.41 0.31 0.21 0.11 0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 Time (secs) Vmax Points = 26 Well Vmax R^2 E2 0.4 64.300 0.984 0.3 0.2 0.1 0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 Time (secs) Vmax Points = 26 Well Vmax R^2 E3 59.852 0.981 [4NPbutyrate] 250 M
0.72 0.22 0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 Time (secs) Vmax Points = 26 Well Vmax R^2 F1 154.84 0.981 0.71 0.21 0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 Time (secs) Vmax Points = 26 Well Vmax R^2 F2 153.86 0.978 0.7 0.2 0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 Time (secs) Vmax Points = 26 Well Vmax R^2 F3 141.80 0.977 [4NPbutyrate] 500 M
0.79 0.59 0.39 0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 Time (secs) Vmax Points = 26 Well Vmax R^2 G1 240.42 0.981 0.87 0.37 0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 Time (secs) Vmax Points = 26 Well Vmax R^2 G2 235.59 0.981 0.95 0.75 0.55 0.35 0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 Time (secs) Vmax Points = 26 Well Vmax R^2 G3 215.45 0.979 [4NPbutyrate] 1000 M
0.9 0.8 0.7 0.6 0 10 20 30 40 50 60 70 80 90 Time (secs) Vmax Points = 26 Well Vmax R^2 H1 297.99 0.992 0.95 0.92 0.75 0.72 0.55 0 10 20 30 40 50 60 70 80 90 0.52 Time (secs) 50 0 10 20 30 40 60 70 80 90 100 Vmax Points = 26 Well Vmax R^2 Well Vmax R^2 H2 Time (secs) 298.14 0.991 272.76 0.982 Vmax Points = 26 H3 0.92 0.72 0.52 0 10 20 30 40 50 60 70 80 90 100 Time (secs) Vmax Points = 26 Well Vmax R^2 H3 272.76 0.982 [4NPbutyrate] 2000 M
