Enzyme assays - PowerPoint PPT Presentation


Understanding Alinity i STAT High Sensitivity Troponin-I for Cardiovascular Diagnosis

Alinity i STAT High Sensitivity Troponin-I is a proprietary assay used in diagnosing myocardial infarction (MI) by quantitatively measuring cardiac troponin I in human plasma. This chemiluminescent microparticle immunoassay (CMIA) aids in the detection of MI, a significant aspect in managing heart d

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Understanding Cell Viability Assays in Laboratory Testing

Cell viability assays play a crucial role in determining the health and status of cells, measuring their ability to survive and proliferate. These assays involve various techniques such as dye exclusion, colorimetric, fluorometric, luminometric, and flow cytometric assays. By assessing factors like

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Enzymes are Proteins that act as Biological Catalyst

Enzymes, as proteins acting as biological catalysts, accelerate chemical reactions without being consumed. They have active sites where substrates bind, and their specificity can be lock-and-key or induced fit. Enzymes are named based on their function or the substrates they interact with. Enzyme sp

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Understanding the Role of Phosphatase Enzymes in Metabolic Reactions

Phosphatase enzymes play a crucial role in various metabolic processes by releasing phosphate groups, increasing their availability for energy synthesis and cell structure formation. Acid phosphatases, with an optimum pH below 7.0, can be extracted from plant tissues like germinating mung beans. A p

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Understanding Enzyme Function Through Induced Fit Model

The learning content discusses enzymes as biological catalysts that increase reaction rates by lowering activation energy. It explores how enzyme activity efficiency is influenced by factors such as temperature, pH, and substrate concentration. The Induced Fit Model is analyzed to explain how enzyme

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Phylogenetic Analysis of Enzyme Sequences in Plant Species

The content discusses the amino acid sequence alignment and phylogenetic tree analysis of enzyme sequences, specifically DcLCYB2, among different plant species including Daucus carota, Carica papaya, Citrus maxima, Solanum lycopersicum, and Crocus sativus. The study explores the genetic relationship

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Protein Assays Market

Protein Assays Market by Type (Dye-based, Copper-based) Technology (Fluorescence, Absorbance, Colorimetric) Offering (Reagent, Kit, Instrument) Application (R&D, Drug Discovery, Diagnostics) End User (Pharmaceutical, Hospital) \u2013 Global Forecast to 2031\n

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Meticulous Research Publishes Comprehensive Report on Multiplex Assays Market

Multiplex Assays Market by Type (Protein [Bead, Planar], Nucleic Acid, Cell) Technology (Flow Cytometry, PCR, Immunoassay) Application (R&D, Diagnosis (Oncology, Cardiovascular, Infectious, Autoimmune Diseases) Product, End User - Global Forecast to 2030\n

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Understanding Bioprocessing: Advantages, History, Applications, and Enzyme Use

Bioprocessing utilizes biological materials for commercial, medical, and scientific purposes, leveraging natural biological activities to develop useful products. This branch of biotechnology offers advantages such as low temperature, pressure, and pH requirements, along with renewable resources for

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Investigating the Impact of Pineapple Enzyme Bromelain on Gelatin and Pasteurization Process

Explore the effects of pineapple enzyme bromelain on gelatin and how pasteurization can alter its effectiveness in this lab experiment. Discover the different outcomes when using fresh versus canned pineapple and formulate hypotheses to guide the investigation. Follow step-by-step procedures to test

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Factors Affecting Enzyme Activity and Catalysis

Enzyme activity is influenced by various factors such as enzyme concentration, temperature, pH, substrate concentration, inhibitors, activators, and physical agents. The rate of enzyme-catalyzed reactions is directly proportional to enzyme concentration, and temperature plays a significant role with

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Understanding Enzyme Function: The Key Steps and Importance of Cofactors

Enzymes play a crucial role in catalyzing biochemical reactions by forming enzyme-substrate complexes and facilitating changes in substrate molecules to product molecules. The process involves four steps: proximity of enzyme and substrate, binding at the active site, catalysis leading to substrate a

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Cell-Based Assays Market

According to a recent report by Market.us, the Global Cell-based Assays Market size is expected to be worth around USD 37.1 Billion by 2032 from USD 16.3 Billion in 2022, growing at a CAGR of 8.8 % during the forecast period from 2023 to 2032.\n\n

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Understanding Peroxidase Enzyme Activity in Biological Samples

Demonstrating the enzyme activity of peroxidase, an enzyme that plays a crucial role in breaking down hydrogen peroxide in various organisms. Learn about the differences between peroxidase and catalase, the calculation of enzyme activity, and the significance of extinction coefficient in enzyme assa

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Understanding Microbial Nutrition and Bacterial Physiology

Microbial nutrition involves essential elements required for microbial growth and energy production. Bacterial physiology delves into the structures and functions that enable bacteria to thrive, from cell wall composition to enzyme activities. Major elements like C, O, H, N, S, and P are crucial for

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Understanding Metabolic Control Mechanisms in Cellular Regulation

Metabolic control mechanisms play a crucial role in maintaining homeostasis within cells by regulating metabolic pathways. This involves finely adjusting the output of pathways in response to external signals, ensuring the proper flux of metabolites to meet cellular needs. Pacemaker enzymes, such as

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GCSE Required Practical Biology Experiments Overview

Explore GCSE Required Practical Biology experiments including using a light microscope, investigating osmosis, conducting food tests, and studying amylase enzyme activity. Understand the objectives, apparatus, results, and potential questions that may be asked in each experiment. Gain insights into

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Understanding Enzymes: Nature, Classification, and Mechanism of Action

Enzymes are biocatalysts synthesized by living cells. They are protein in nature and specific in their action. This article delves into the definition of enzymes, classification based on enzymatic action, enzyme class reactions, structure, and mechanism of enzyme action, highlighting how enzymes pla

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Exploring Enzyme Kinetics for Understanding Chemical Reactions

Enzyme kinetics is a vital discipline focusing on the rate of enzyme-catalyzed reactions and how they respond to varying conditions. Reactions are classified based on reactant concentration influences. Zero, first, second, and third order reactions are distinguished, with examples like first-order r

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Demonstration of Salivary Amylase Enzyme Action in B.Sc. Practical

Salivary amylase, an enzyme found in saliva, partially hydrolyzes starch into maltose. This practical involves observing the action of salivary amylase on starch, demonstrating how starch is broken down into glucose and maltose. The procedure includes preparing solutions, collecting saliva, mixing w

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Enzyme Immobilization: Techniques and Applications in Industry and Healthcare

Enzyme immobilization involves confining enzymes on inert supports for stability and reuse, enhancing efficiency and cost-effectiveness. Historical events and examples illustrate various methods and applications. Chemical modifications, such as PEG addition, have shown increased enzyme activity. The

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Factors Affecting Enzyme Activity and Kinetics Experiments

Explore the factors influencing enzyme activity, such as substrate and enzyme concentration, temperature, pH, and inhibitors. Learn how to simulate enzyme kinetics using equipment like popping beads and stopwatches. Analyze results to understand the impact of substrate concentration on reaction rate

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Understanding Enzyme Activity and Optimal Conditions

This interactive content provides a detailed exploration of enzyme activity through data interpretation and graph analysis. Questions range from identifying the impact of enzymes on specific molecules to determining optimal conditions for various enzyme functions such as pH and temperature. Users de

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Immobilization of Enzymes in Biochemistry

Enzyme immobilization involves confining enzyme molecules to a distinct phase from substrates and products, attaching them to solid matrices for enhanced specificity and reduced inhibition. Inert polymers or inorganic materials are used as carrier matrices with methods like physical adsorption onto

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Understanding Enzyme Inhibition in Biochemistry

Enzyme inhibition plays a crucial role in pharmacology and biochemistry by regulating enzymatic reactions. Inhibitors can be reversible or irreversible, affecting enzyme activity differently. Competitive, uncompetitive, and noncompetitive inhibition types are explained along with examples like diiso

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Plant Enzyme Activities and Phylogenetic Analysis

The content discusses the alignment of various plant enzyme amino acid sequences, including DcLCYB1, DcLCYB2, and DcLCYE, among different inbred lines. It also explores the construction of DcLCYs for enzyme activities using an E. coli complementation assay. The phylogenetic relationship between DcLC

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Global Cell-Based Assays Market

The Global Cell Based Assays Market Size is Anticipated to Exceed USD 35.42 Billion by 2033, Growing at a CAGR of 7.47% from 2023 to 2033.\n

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Demonstration of Salivary Enzyme Amylase Action in B.Sc. Practical

Salivary enzyme amylase, also known as ptyalin, plays a crucial role in breaking down starch and glycogen into maltose. This practical session in the Zoology department explores the action of salivary enzyme amylase at a temperature of 37°C and pH of 6.6. By conducting experiments with starch, iodi

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Understanding Enzyme Inhibition in Acid Phosphatase Kinetics

In this experiment, the effect of inhibitors on enzymatic reactions, specifically acid phosphatase, is explored. Different types of inhibition (competitive, noncompetitive, uncompetitive) are examined, along with reversible and irreversible inhibitors. The study aims to determine the type of inhibit

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Understanding Enzyme Assays in Biotechnology

Exploring enzyme assays in biotechnology, covering topics such as ELISA, protein assays, enzyme activity measurement techniques, specific activity, terminology related to enzyme assays, and different types of experiments conducted by biochemists to study enzyme-catalyzed reactions. The content delve

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Antibody Production for Phenylbutazone and Oxyphenylbutazone Detection Assays in Veterinary Sciences

Terry Fodey, from the Veterinary Sciences Division in the UK, focuses on producing antisera for phenylbutazone and oxyphenylbutazone to use in immunochemical detection assays. The presentation covers the importance of antibodies, approaches to production, and performance comparison. Phenylbutazone,

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Cyanide's Impact on Respiration Through Enzyme Inhibition

Cyanide, a noncompetitive inhibitor of the enzyme cytochrome c oxidase, disrupts electron transport in respiration by binding to the iron cofactor and blocking electron transfer to oxygen. This lethal effect was utilized in gas chambers during WWII, underscoring the crucial role of enzyme function i

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Understanding Multisubstrate Enzyme Kinetic Mechanisms

In multisubstrate enzyme kinetic mechanisms, the apparent Km and Vmax values change with varying substrate concentrations. Different kinetic mechanisms like rapid equilibrium Bi Bi and ordered Bi Bi reactions can occur. The ping-pong Bi Bi reaction involves oscillation between enzyme forms. Various

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Factors Affecting Polyphenol Oxidase Activity in Enzyme Reaction

Polyphenol oxidase (PPO) is a copper-containing enzyme with an optimal pH of 6.7 that catalyzes the oxidation of phenols, leading to color changes like browning in fruits and potatoes. This experiment aims to demonstrate PPO activity, its chemical nature, substrate specificity, and the effects of te

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Understanding Factors Affecting Enzyme Activity in Biochemistry

Enzyme assays measure substrate conversion to product under varying conditions like cofactors, pH, and temperature. Enzyme velocity represents the rate of a catalyzed reaction, typically reported as V0. Enzyme activity is expressed as mol of substrate transformed per minute, with enzyme unit and kat

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Enzymatic Digestion of Fat by Pancreatic Lipase

The experiment focuses on studying the enzymatic digestion of fat by pancreatic lipase. It covers the structure of triglycerides, the role of lipase enzyme in hydrolyzing triglycerides to release fatty acids, and the general hydrolysis process. The aim is to investigate the effects of lipase enzyme

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Understanding LacZ - Galactosidase Enzyme: Structure, Function, and Applications

LacZ - galactosidase enzyme, encoded by the lacZ gene in the lac operon, plays a crucial role in cleaving lactose, transgalactosylation, and regulating gene expression. Its application in labs using X-gal as a substrate allows for easy detection of active enzyme through color changes. This versatile

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Nonparametric Tolerance Interval Approach to Immunogenicity Assay Cut Point Determination

This content discusses the importance of determining the cut point for immunogenicity assays to detect anti-drug antibodies (ADA) in large-molecule therapeutics. It highlights the need for well-developed and validated assays to ensure drug efficacy and safety. The approach involves multiple-tiered v

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Protein Purification Protocol: Practical Training in Biochemistry Techniques

This protein purification protocol course, led by Dr. Samina Hyder Haq, focuses on practical training in various biochemistry techniques. The course covers organizing experimental protocols, protein isolation strategies, sequential enzyme purification, enzyme kinetics, and writing scientific reports

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Understanding Enzyme Regulation and Factors Affecting Enzyme Activity

Organisms carefully control enzyme production and activation as per varying needs and conditions within cells. Enzyme activity is influenced by factors such as pH, temperature, regulatory molecules, cofactors, compartmentalization, covalent modification, and feedback inhibition. Enzymes can be regul

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