BAG/LTP Submissions and Proposals Timeline
Detailed outline of timeframes and requirements for BAG/LTP submissions, proposals, and reports at eBIC MX and XChem, emphasizing the importance of timely requests, clear justifications for instrument usage, and regular reporting intervals to maintain allocation periods. The timeline example showcases the sequential process for submitting, requesting, and reporting time allocations for multiple advanced photonics (AP) periods, ensuring efficient utilization of resources for scientific research.
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BAG/LTP SUBMISSIONS: Outline of timeframes (first couple of slides) valid for all Specific information for eBIC MX and XChem
BAG/LTP Proposals AP35 AP36 Jan Feb Mar Apr May Jun Jul Aug Sept Oct Nov Dec AP36 time request AP37 time request BAG proposals last 4 allocation periods (AP) if taken consecutively would be 2 years PIs/ACs need to submit report + time request for next AP every 6 months Note the requested time should be for the 6 month period only Proposal/request deadline 3rd April 2024 New BAGs/LTPs and continuation BAGs should upload a new science case. This stays on the proposal for the lifecycle of the BAG/LTP (i.e., for all 4 APs), unless changes are necessary For each instrument requested make a clear case for why it is needed Select the instrument needed, check the beamline accepts BAGs/LTPs here To request Krios and Aquilos time, select the eBIC + Aquilos instruments To request MX time select MX as the instrument, I23 (long wavelength), I24 (tuneable microfocus and SSX) and VMXi (in situ room temperature), you may also need B21 (BioSAXS) NOTE: MX proposals should always include at least 1 shift on I24, I23 and VMXi
BAG Proposal Timeline (example, with continuations) Report submission Deadline Submission AP (6 month period) Aim Required AP30 New proposal requesting time for AP32-35 AP31 Request time for AP33 AP32 Request time for AP34 Submit 6-month report for AP32 27.09.23 03.04.24 AP33 Request time for AP35 Submit 6-month report for AP33 AP34 Submit continuation proposal for AP36-39 Submit 18-month report for AP32-34 25.09.24 AP35 Request time for AP36 Submit 6-month report for AP35 26.03.25 AP36 Request time for AP37 Submit 6-month report for AP36 24.09.25 AP37 Request time for AP38 Submit 6-month report for AP37 25.03.26 AP38 Submit continuation proposal for AP39-42 Submit 18-month report for AP36-38 23/30.09.26 AP39 Request time for AP40 Submit 6-month report for AP39
BAG Proposals - Request from Reviewers Please provide three highlights, even if not yet published (proposals are confidential) If you copy your proposal for a new (continuing) BAG, please check all information is still relevant, incl. investigators and grants remove any that are no longer relevant or grants that have expired - Rember to save your changes Don t list collaborators as BAG PIs. Only list active members who have projects Collaborators should be included in science justification of active PIs Be explicit about projected usage of each of the instruments required by each PI in BAG. At end of each project description, add a line to say how much specific instrument time is required for the AP being submitted for Provide sufficient preliminary data to aid assessment of the readiness and technical feasibility of project. Provide detailed summary of how the sessions in the previous AP were used: E.g. (eBIC) number of samples imaged, number of movies/tilt series collected, resolution obtained, project stage including resolution obtained. Be clear about output: structures/maps/publications and AP of session generating the results.
eBIC Please could BAG managers (PI and ACs) avoid duplicating project descriptions that are unchanged from the original science case. Please check continued relevance. There is no need to include science case information in your report/request for time requested at 6 month reporting. The panel is concerned about duplicate information arising. For projects requesting eBIC time for either or both SPA/tomography and cryoFIB-SEM lamella preparation should include as much detail as reasonably possible, with associated preliminary data, to ensure reviewers can accurately assess them. In particular for cryoFIB-SEM lamella preparation: PI requests should include data such as cryoLM/TEM atlases of cells grown on grids showing a reasonable distribution of cells. In addition, if the target protein is fluorescently labelled then cryoFM or room temperature confocal data should also be provided New proposals Please provide evidence of cells grown on grids/sample distribution on grid and ice thickness (e.g. atlas and grid square overview). If you are planning to use fluorescence, please include fluorescent imaging data either at RT or cryo temperatures depending on available instrumentation. For renewal or extension of cryoFIEB-SEM lamella projects Please provide evidence on how your last beamtime was used. How successful was it? e.g. how many lamallae/tomograms were collected. Do include how you analysed the data and what you got out of it. Indicate whether you were able to detect the features you wanted to analyse and include images/results. If the session was unsuccessful, explain what when wrong and how you modified your sample to mitigate the issues encountered, especially if the issue was sample based. Please also include an explanation of why you need more time.
MX submissions MX submissions MX Proposals - For MX proposals request time on all available instruments (MX, I24, I23, VMXi) in anticipation that you may have projects that arise in which these beamlines would be advantageous to use during the allocation period, and to provide operational flexibility to meet your needs. For SAXS time on B21 see below. B21 SAXS - The review panels request that MX BAG proposals provide sufficient specific detailed justification for their request for B21 beamtime. The BAG science case in the User Administration System should be used; developing this will strengthen your science case for bioSAXS time. VMXi is available for remote access, offering in situ room temperature data collection. The beamline has a 10x10 micron pink beam allowing data to be measured to ~1.5 resolution with automatic data processing and merging of datasets using xia2.multiplex within the usual ISPyB interface. VMXi offer grid scans to identify crystals and their diffraction quality. Users may bring crystals in crystallisation plates to Diamond for data collection or bring a protein sample to set up crystallisation on site working with our on-site experts. Please contact the VMXi team (michael.hough@diamond.ac.uk) to discuss the available options.
XChem XChem submissions submissions an XChem BAG Science Case using the template and an XChem Six Month BAG Report (or an XChem Final Review Report if at the end of a two year BAG cycle). For details on submitting XChem applications please refer to the web-page and contact the XChem team if you have any questions.
