Understanding Chromatography in Biochemistry: A Visual Guide
Explore the world of chromatography through a series of informative images covering principles, classifications, procedures, and significance of techniques like paper chromatography and thin-layer chromatography (TLC). Delve into the R value, two-dimensional chromatography, and learn about partition chromatography. Enhance your knowledge with detailed visuals and descriptions.
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Presentation Transcript
CHROMATOGRAPHY Biochemistry of Medics 1
R value 14
Adsorption chromatography The substance loosely adsorbed to the stationary medium comes in the earlier fraction. 24
Ion Exchange chromatography- Procedure (Contd.) 28
Ion Exchange chromatography- An Overview Biochemistry of Medics 30
4) Gel filtration chromatography or Molecular Sieve chromatography 31
Gel filtration chromatography or Molecular Sieve chromatography 32
Gel filtration chromatography-An Overview Larger particles come out first, while smaller particles come in later fractions 33
Affinity chromatography- Significance By using antibodies, antigens can be easily separated. Conversely, antibodies can be purified by passing through a column containing the antigen. Specific Antibody binds to a specific antigen attached to the stationary medium, the remaining antibodies come out in earlier fractions. 36 Biochemistry of Medics
High performance liquid chromatography- Significance 39
High performance liquid chromatography- Apparatus Biochemistry of Medics 40
Gas liquid chromatography-Apparatus Biochemistry of Medics 43
Summary 44
