Overview of Gene Transfer Methods in Molecular Biology

Explore various physical and chemical methods used for gene transfer, including electroporation, particle bombardment, microinjection, ultrasound, and bacterial transformation. Learn how DNA enters the nucleus and the role of retroviruses in gene transfer efficiency. Understand the process of RNA containing gene sequences of interest transduction. Visual aids and explanations enhance comprehension of complex molecular processes.

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• Gene Transfer
• Molecular Biology
• Electroporation
• Retroviruses
• DNA Transduction

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Uploaded on Mar 19, 2024 | 0 Views

Presentation Transcript

1. Methods of gene transfer

2. Physical gene transfer methods Electroporation Optimized

3. Particle bombardment

4. Microinjection

5. Ultrasound method

6. Chemical gene transfer methods

7. PMMA DEAE (Polymer beads) DNA Endocytosis occurs

11. BACTERIAL TRANFORMATION

14. How DNA enters the nucleus from cytoplasm?

15. Retroviruses

16. Retrovirus mediated gene transfer Why retrovirus??? They have better recognition efficiency by different host cells

17. RNA containing gene sequence of interest Transduction cDNA cDNA