Understanding Laminar Air Flow System
Learn about the principle and working of Laminar Air Flow (LAF) systems, which are essential for reducing contamination when working with microorganisms. The system utilizes High Efficiency Particulate Air Filters (HEPA) to prevent the passage of small microbial cells, maintaining a clean environment. Follow the detailed procedure and precautions outlined to ensure proper operation of the LAF system and minimize the risk of contamination.
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Presentation Transcript
Aim : To demonstrate the principal and working of Laminar Air Flow.
Principle: It is used for reducing the degree of infection while working with infectious microorganism and for preventing contamination of sterile material. It works on the principle of application of High Efficiency Particulate Air Filter (HEPA filter) that contains very small openings or pores. So, that small microbial cells cannot pass through them on air filtration. Room air is filtered before entering the working chamber and moves in a single direction.
Procedure : 1. Switch on the LAF and switch on the blower and fluorescent 2. light. 3. Wipe LAF with the help of cotton dipped in 70% ethanol. 4. Switch on UVlight for 15 minutes. So as to kill the germs. 5. After 15 minutes switch off the UV light and turn on the 6. blower and the fluorescent light. 7. Light the Bunsen burner and continue the work required 8. around the Bunsen burner.
Precautions: Wash the hands with soap before entering the LAF chamber. Wipe your hands with 70% ethanol before working. Remove all the accessories of hand and do not talk while working.
