Understanding Chromatography in Biochemistry: A Visual Guide

Explore the world of chromatography through a series of informative images covering principles, classifications, procedures, and significance of techniques like paper chromatography and thin-layer chromatography (TLC). Delve into the R value, two-dimensional chromatography, and learn about partition chromatography. Enhance your knowledge with detailed visuals and descriptions.

• Chromatography
• Biochemistry
• Techniques
• Paper Chromatography
• Thin-Layer Chromatography

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Uploaded on Aug 07, 2024 | 2 Views

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Presentation Transcript

1. CHROMATOGRAPHY Biochemistry of Medics 1

2. Chromatography 2

3. Chromatography 3

4. Chromatography- Principle 4

5. Chromatography- Classification 5

6. Chromatography- Classification(Contd.) 6

7. Partition Chromatography 7

8. Paper chromatography 8

9. Paper chromatography 9

10. Paper chromatography- Procedure 10

11. Paper chromatography- Procedure 11

12. Ascending and Descending Paper Chromatography 12

13. Paper Chromatography -Analysis 13

14. R value 14

15. Two dimensional chromatography 15

16. Significance of Paper chromatography 16

17. Thin layer chromatography (TLC) 17

18. TLC- Procedure 18

19. TLC -Overview 19

20. Significance of TLC 20

21. Advantages of TLC over paper chromatography 21

22. 2)Adsorption chromatography 22

23. 2)Adsorption chromatography- Elution 23

24. Adsorption chromatography The substance loosely adsorbed to the stationary medium comes in the earlier fraction. 24

25. Ion Exchange chromatography 25

26. Ion Exchange chromatography 26

27. Ion Exchange chromatography- Procedure 27

28. Ion Exchange chromatography- Procedure (Contd.) 28

29. Types of ion exchanges resins 29

30. Ion Exchange chromatography- An Overview Biochemistry of Medics 30

31. 4) Gel filtration chromatography or Molecular Sieve chromatography 31

32. Gel filtration chromatography or Molecular Sieve chromatography 32

33. Gel filtration chromatography-An Overview Larger particles come out first, while smaller particles come in later fractions 33

34. 5) Affinity chromatography 34

35. Affinity chromatography- Significance 35

36. Affinity chromatography- Significance By using antibodies, antigens can be easily separated. Conversely, antibodies can be purified by passing through a column containing the antigen. Specific Antibody binds to a specific antigen attached to the stationary medium, the remaining antibodies come out in earlier fractions. 36 Biochemistry of Medics

37. 6) High performance liquid chromatography- 37

38. High performance liquid chromatography- 38

39. High performance liquid chromatography- Significance 39

40. High performance liquid chromatography- Apparatus Biochemistry of Medics 40

41. 6) Gas liquid chromatography- 41

42. Gas liquid chromatography- 42

43. Gas liquid chromatography-Apparatus Biochemistry of Medics 43

44. Summary 44