Tannins: Properties, Classification, and Medicinal Uses
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Secondary metabolite Products , naturally occurring complex organic
compounds. Nitrogen-free polyphenols with high molecular weight.
Precipitation of proteins and alkaloids. The property of tannins as an
astringent is due to their ability to precipitate proteins and make
them resistant for enzymatic attack.
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Soluble in water (except for some highweight structures molecular), alcohols,
dilute alkalis, and glycerin.
2.
Insoluble in organic solvents, except acetone, slightly soluble in ethyl acetate.
3.
It tastes bitter if the tannin content is high .
4.
Color is yellowish white to brown .
5.
High molecular weight ranges from 500 to >5,000 mw.
6.
Form a non-crystallizable colloidal solution with water which leads to acidic
reactions.
7.
It has an astringent property and astringent taste in the mouth.
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Tannin compounds can be divided into two main groups based on the Goldbeater skin
test
:
1-
True tannins
:
A group of tannins that show a positive Goldbeater skin test , high
molecular weight(>500mw).
2-
Pseudo-tannins
:
They are simple phenolic compounds with lower molecular
weight(<500mw).It does not respond to the tanning reaction of the Goldbeater skin test
.
Examples: Cocoa , Coffee
.
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Tannins can be divided into two main groups based on the chemical structure and hydrolytic reaction
:
1-
Hydrolysable tannins
:
They are ester of sugar and variable number of phenolic acid, they are
hydrolysed by acids or enzyme , and produced Gallic acid and ellagic acid, soluble in water and their
solution produces a blue color with ferric chloride.
Examples: pomegranate , eucalyptus .
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Condensed
or
Nonhydrolysable tannins
:
They
are not readily hydrolysable in to simpler
molecules , not contain sugar moity ,polymeric flvans , condensed tannins are also soluble in water and
produce a green color with ferric chloride.
Examples: Kola , Green tea .
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1.
Astringent for burns and inflammation of mucous membranes.
2.
Antioxidant, antibacterial, antifungal , anticancer .
3.
Tannins are used to treat skin ulcers, hemorrhoids, frostbite, as well as inflammation of
gums.
4.
Stomach stimulant, blood thinner, diuretic.
5.
Sore throat, diarrhea, dysentery .
6.
Treatment for bee sting venom.
7.
Control gastroenteritis, and in cases of heavy metal poisoning as an antidot.
8.
In recent years, these compounds have proven their antiviral activity in treating viral
diseases, including... AIDS.
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It is used in the leather tanning process.
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Protect plants from herbivores.
3.
Protect plants from microbial attacks.
4.
Tannins are used as an adhesive in dyeing, ink making, silk.
5.
Tannins are also used in photography, and as a coagulant in the
rubber industry.
6.
Detector in laboratories.
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Eucalyptus
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S.name:
Eucalyptus camaldulenses
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Family : Myrtaceae
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active part : Leaves and bark
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Pomegranate
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S.name :
Punica granatum
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Family: Punicaceae
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active part : Peels
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Prepare plant extract :
1gm of plant in 200ml D.W (decoction method).
1
-
gelatin
test :
1 ml of the extracts mixed with few drops of 1% gelatin
solution ; the mixture gives white precipitate ,
(
gelatin is protein).
2
-
Lead acetate tests:
1ml of extracts mixed with few drops 10% lead
acetate ; the mixture gives white precipitate.
3
-
Ferric chloride (FeCl3) tests (
Hydrolyzable tannins):
1ml of the
extracts mixed with few drops of 5%
Ferric chloride the mixture gives blue
to black color.
Tannins are secondary metabolite products with astringent properties, high molecular weight, and various solubility and taste characteristics. They can be classified into true tannins and pseudo-tannins based on the Goldbeater skin test, as well as hydrolysable and condensed tannins based on chemical structure. Tannins have medicinal properties such as acting as antioxidants, antibacterial agents, and aids in treating various conditions including burns, inflammation, ulcers, and throat infections.
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Presentation Transcript
TANINS Assistant lecturer Ola Alsaad
TANNINS Secondary metabolite Products , naturally occurring complex organic compounds. Nitrogen-free polyphenols with high molecular weight. Precipitation of proteins and alkaloids. The property of tannins as an astringent is due to their ability to precipitate proteins and make them resistant for enzymatic attack.
Properties of tannins 1. Soluble in water (except for some highweight structures molecular), alcohols, dilute alkalis, and glycerin. 2. Insoluble in organic solvents, except acetone, slightly soluble in ethyl acetate. 3. It tastes bitter if the tannin content is high . 4. Color is yellowish white to brown . 5. High molecular weight ranges from 500 to >5,000 mw. 6. Form a non-crystallizable colloidal solution with water which leads to acidic reactions. 7. It has an astringent property and astringent taste in the mouth.
Classification of tannins Tannin compounds can be divided into two main groups based on the Goldbeater skin test : 1- True tannins : A group of tannins that show a positive Goldbeater skin test , high molecular weight(>500mw). 2- Pseudo-tannins :They are simple phenolic compounds with lower molecular weight(<500mw).It does not respond to the tanning reaction of the Goldbeater skin test. Examples: Cocoa , Coffee .
Classification of tannins Tannins can be divided into two main groups based on the chemical structure and hydrolytic reaction : 1- Hydrolysable tannins : They are ester of sugar and variable number of phenolic acid, they are hydrolysed by acids or enzyme , and produced Gallic acid and ellagic acid, soluble in water and their solution produces a blue color with ferric chloride. Examples: pomegranate , eucalyptus . 2- Condensed or Nonhydrolysable tannins : They are not readily hydrolysable in to simpler molecules , not contain sugar moity ,polymeric flvans , condensed tannins are also soluble in water and produce a green color with ferric chloride. Examples: Kola , Green tea .
Medicinal properties and uses 1. Astringent for burns and inflammation of mucous membranes. 2. Antioxidant, antibacterial, antifungal , anticancer . 3. Tannins are used to treat skin ulcers, hemorrhoids, frostbite, as well as inflammation of gums. 4. 5. 6. Stomach stimulant, blood thinner, diuretic. Sore throat, diarrhea, dysentery . Treatment for bee sting venom. 7. Control gastroenteritis, and in cases of heavy metal poisoning as an antidot. 8. In recent years, these compounds have proven their antiviral activity in treating viral diseases, including... AIDS.
Other uses of tannins 1. It is used in the leather tanning process. 2. Protect plants from herbivores. 3. Protect plants from microbial attacks. 4. Tannins are used as an adhesive in dyeing, ink making, silk. 5. Tannins are also used in photography, and as a coagulant in the rubber industry. 6. Detector in laboratories.
Tanins example Pomegranate Eucalyptus S.name : Punica granatum S.name:Eucalyptus camaldulenses Family: Punicaceae Family : Myrtaceae active part : Peels active part : Leaves and bark
The procedure Prepare plant extract : 1gm of plant in 200ml D.W (decoction method). 1- gelatin test : 1 ml of the extracts mixed with few drops of 1% gelatin solution ; the mixture gives white precipitate , (gelatin is protein). 2- Lead acetate tests: 1ml of extracts mixed with few drops 10% lead acetate ; the mixture gives white precipitate. 3-Ferric chloride (FeCl3) tests (Hydrolyzable tannins): 1ml of the extracts mixed with few drops of 5% Ferric chloride the mixture gives blue to black color.
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