Shigella: Characteristics, Tests, and Diseases
metalic green on
( EMB )
Shigella spp.
Shigella spp.
on
on
HE
HE
(
(
blue
blue
-
-
green
green
)
)
Biochemical test
(TSI) test :
Alk slant (-) ve
*
Acid butt (+) ve
No gas production ,
no
H2S produced
.
IMVC :
v + - -
Urease :
(-) ve
Nitrate reduction : (+) v
e
Shigella
•
Shigella
10-100
•
C.jejuni
10
2
-10
6
•
Salmonella
10
5
•
E.coli
10
8
•
V.cholerae
10
8
•
G.lamblia
10-100 cysts
•
E.histolytica
10-100 cysts
•
C.parvum
1-1000 cysts
•
Bloody diarrhae containing mucus,
•
Shigatoxin-an exotoxin B unit binds to host cell
glycolipids and A cleaves the 28S rRNA ,
preventing the protein synthesis
•
Shigatoxin can mediate the damage to the
glomeruşar endothelial calls, resulting in renal
failure (HUS).
Shigella-
Shigella-
Diseases
Diseases
Shigella-
Epidemiology
•
Humans are only reservoir for these bacteria
•
Disease spread person to person by fecal-oral route
•
Patients at highest risk for disease are young children
in daycare centers, nurseries, male homosexuals
•
Relatively few organisms can produce disease (highly
infectious)
•
Disease occurs worldwide with no seasonal incidence
Shigella
-Treatment, Prevention, and Control
•
Antibiotic therapy shortens the course of
symptomatic disease and fecal shedding
•
Treatment should be guided by in vitro susceptibility
tests
•
Empiric therapy can be initiated with a
fluoroquinolone or trimethoprim-sulfamethoxazole
•
Appropriate infection control measures should be
instituted to prevent spread of the organism
Thank
Thank
you
you
Exploring the characteristics of Shigella bacteria through differential media, biochemical tests, and antigen identification. Learn about the diseases associated with Shigella infections, including the transmission methods and high-risk groups. Discover how Shigatoxin plays a role in Shigella-related illnesses.
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Presentation Transcript
MacConkey Agar metalic green on ( EMB ) Differential Media: left: no lactose fermentation right: lactose fermentation
Shigella spp. on HE (blue - green)
Biochemical test (TSI) test : Alk slant (-) ve* Acid butt (+) ve No gas production , no H2S produced . IMVC : v + - - Urease : (-) ve Nitrate reduction : (+) ve
Shigella O antigen Ornithin decarboxylase Lactose S.dysentariae A - - S.flexneri B -- - S.boydii C - - S.sonnei D + - Late+
Shigella C.jejuni Salmonella E.coli V.cholerae G.lamblia E.histolytica C.parvum 10-100 102-106 105 108 108 10-100 cysts 10-100 cysts 1-1000 cysts
Shigella-Diseases Bloody diarrhae containing mucus, Shigatoxin-an exotoxin B unit binds to host cell glycolipids and A cleaves the 28S rRNA , preventing the protein synthesis Shigatoxin can mediate the damage to the glomeru ar endothelial calls, resulting in renal failure (HUS).
Shigella-Epidemiology Humans are only reservoir for these bacteria Disease spread person to person by fecal-oral route Patients at highest risk for disease are young children in daycare centers, nurseries, male homosexuals Relatively few organisms can produce disease (highly infectious) Disease occurs worldwide with no seasonal incidence
Shigella-Treatment, Prevention, and Control Antibiotic therapy shortens the course of symptomatic disease and fecal shedding Treatment should be guided by in vitro susceptibility tests Empiric therapy can be initiated with a fluoroquinolone or trimethoprim-sulfamethoxazole Appropriate infection control measures should be instituted to prevent spread of the organism
Thank you Thank you
