Salmonella: A Zoonotic Pathogen
Dr. Ban sahib abed al-nabi
zoonotic disease unit
post graduate lecture
Salmonella
Genus : Salmonella
Salmonella as pathogens
Salmonella
species are facultative
intracellular pathogens
.
Many
infections are due to ingestion of
contaminated
food.
Salmonella
serovars can be
divided into two main groups—
typhoidal and
nontyphoidal
Salmonella
.
Nontyphoidal serovars are more
common, and usually cause self-
limiting gastrointestinal disease. They
can infect a range of animals, and
are
zoonotic
, meaning they can be
transferred between humans and
other animals.
Morphology & S taining :
It is a Gram negative bacteria , cocco bacilli or straight
rod with rounded ends .
some times they develop into pleomorphic forms or
very short coccobacilli after prolonged culture on
media .
Micro scopic appearance & Incubation period :
Natural
Habitat
•
Salmonellae
live in the intestinal tracts of warm and cold
blooded animals. Some species are ubiquitous. Other
species are specifically adapted to a particular host.
•
In humans,
Salmonella
are the cause of two diseases called
salmonellosis
:
–
enteric fever
(
typhoid
),
resulting from bacterial invasion of the
bloodstream
–
acute gastroenteritis
,
resulting from a foodborne infection .
–
The majority of infected animals become subclinical excretors .
–
Salmonella can survive for 9 months or more in the
environment such as moist soil , water , faecal & animal feeds .
Isolation & Identification of salmonella :
1.Enrichment broth
Mannitol Selenite broth & Tetera thionate
broth & Soy peptone broth
for salmonella
isolation from stool specimen .
These fluid a useful medium which allows the
growth of salmonella but inhibits other enteric
organisms .
1.
MacConkey agar & desoxy cholate citrate agar :
(DCA)
The medium & colonies are pale yellow- straw
smooth &
non lactose Ferment .
lactose fermenting Non lactose Fermenting
•
Escherichia coli Salmonella spp
•
Klebsiella pneumoniae Shigella
proteus
2.
Salmonella- Shigella agar (ss agar) :
Its selective for salmonella .
Colonies are colorless
with black center .
Non lactose ferment .
3.
Xylose lysine desoxy cholate (XLD) :
Red colonies
with black centers of H2S
producing . Non lactose ferment .
4.
Brilliant green agar :
Red colonies & medium
. Non lactose ferment .
Smooth circular entire edges .
5.
Bismuth sulfite agar :
Is highly selective for salmonella & one of the
most sensitive media to detect H2S production.
Colonies are black surrounding by
metallic sheen
on the surface.
6.
Hektoen enteric agar (HE) :
Colonies are
blue
-
Green
with black due to H2S
production. Non lactose ferment .
Biochemicals test :
•
salmonella don’t ferment lactose, sucrose but
ferment glucose, maltose , mannitol .
•
•
•
positive Negative
simmon citrate +
TSI/ p/y +H2S
M
o
t
i
l
i
t
y
t
e
s
t
/
m
o
t
i
l
+
H
2
S
VP/ Negative
MR/ positive
Negative
urease test -
Salmonella, a genus of bacteria, includes species causing various diseases in humans, cattle, sheep, goats, and poultry. The pathogen can be transmitted through contaminated food and is classified into typhoidal and nontyphoidal serovars. Nontyphoidal serovars are common and typically lead to self-limiting gastrointestinal issues. Salmonella's Gram-negative morphology, including pleomorphic forms, and its incubation period in different environments contribute to its pathogenic nature.
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Salmonella Dr. Ban sahib abed al-nabi zoonotic disease unit post graduate lecture
Species In human Diseases 1. S. typhi Causes typhoid fever 2. S. para typhi causes para typhoid fever 3. S. enteritidis causes food poisoning 4. S. typhimurium causes food poisoning In cattle & sheep Diseases 1. S. dublin Enteritis & septicaemia 2. S. typhimurium Enteritis & septicaemia
Species in goat Disease 1. S. typhimurium 2. S. enteritidis 3. S. hadar Species in poultry & bird Disease 1. S. pullorum 2. S. gallinarum 3. S. arizonae causes enteritis = = causes white diarrhoea causes fowl typhoid causes enteritis,septicaemia = = 4. S. enteritidis 5. S. typhimurium
Salmonella as pathogens Salmonella species are facultative intracellular pathogens .Many infections are due to ingestion of contaminated food. Salmonella serovars can be divided into two main groups typhoidal and nontyphoidal Salmonella.
Nontyphoidal serovars are more common, and usually cause self- limiting gastrointestinal disease. They can infect a range of animals, and are zoonotic, meaning they can be transferred between humans and other animals.
Morphology & S taining : It is a Gram negative bacteria , cocco bacilli or straight rod with rounded ends . some times they develop into pleomorphic forms or very short coccobacilli after prolonged culture on media .
Micro scopic appearance & Incubation period : G ram stain Negative Morphology cocco bacilli or straight rod with rounded end Motility by peritrichous flagella (actively motile) except S. pullorum & S. gallinarum Capsule observed with mucoid strains Spore Non Temperature 37 c on media without any addition Time 16- 24 hours Atmosphere Aerobic & facultative an aerobic
Natural Habitat Salmonellae live in the intestinal tracts of warm and cold blooded animals. Some species are ubiquitous. Other species are specifically adapted to a particular host. In humans, Salmonella are the cause of two diseases called salmonellosis: enteric fever (typhoid), resulting from bacterial invasion of the bloodstream acute gastroenteritis, resulting from a foodborne infection . The majority of infected animals become subclinical excretors . Salmonella can survive for 9 months or more in the environment such as moist soil , water , faecal & animal feeds .
Isolation & Identification of salmonella : 1.Enrichment broth Mannitol Selenite broth & Tetera thionate broth & Soy peptone broth for salmonella isolation from stool specimen . These fluid a useful medium which allows the growth of salmonella but inhibits other enteric organisms .
1. MacConkey agar & desoxy cholate citrate agar : (DCA) The medium & colonies are pale yellow- straw smooth & non lactose Ferment . lactose fermenting Non lactose Fermenting Escherichia coli Salmonella spp Klebsiella pneumoniae Shigella proteus
2. Salmonella- Shigella agar (ss agar) : Its selective for salmonella . Colonies are colorless with black center . Non lactose ferment .
3. Xylose lysine desoxy cholate (XLD) : Red colonies with black centers of H2S producing . Non lactose ferment .
4. Brilliant green agar : Red colonies & medium . Non lactose ferment . Smooth circular entire edges .
5. Bismuth sulfite agar : Is highly selective for salmonella & one of the most sensitive media to detect H2S production. Colonies are black surrounding by metallic sheen on the surface.
6. Hektoen enteric agar (HE) : Colonies are blue-Green with black due to H2S production. Non lactose ferment .
Biochemicals test : salmonella don t ferment lactose, sucrose but ferment glucose, maltose , mannitol . positive Negative
TSI/ p/y +H2S simmon citrate + Motility test/ motil +H2S
MR/ positive VP/ Negative Negative urease test -
