Molecular Biology Study: Primer Sequences and Correlation Analyses

This study delves into the realm of molecular biology, focusing on primer sequences for various genes like IL13, IL33, Muc5ac, and more. Additionally, it explores PCA analyses of different study groups and correlation of variables using Pearson correlation coefficient. The research sheds light on the intricate connections within biological processes.

• Molecular Biology
• Primer Sequences
• Correlation Analysis
• PCA
• Genes

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Uploaded on Jul 16, 2024 | 0 Views

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1. Name Forward Sequence Reverse Sequence Tslp GCAAATCGAGGACTGTGAGAGC TGAGGGCTTCTCTTGTTCTCCG IL13 AACGGCAGCATGGTATGGAGTG TGGGTCCTGTAGATGGCATTGC IL33 CTACTGCATGAGACTCCGTTCTG AGAATCCCGTGGATAGGCAGAG 18s GGTGGAGTCATACGCATTCTGG ACAGCATTCCCAGTGCCTTGGA IL6 TACCACTTCACAAGTCGGAGGC CTGCAAGTGCATCATCGTTGTTC Kc TCCAGAGCTTGAAGGTGTTGCC AACCAAGGGAGCTTCAGGGTCA Muc5ac CCACTTTCTCCTTCTCCACACC GGTTGTCGATGCAGCCTTGCTT Muc5B CTGAAGACCTGTCGGAACCCAA GCCACACACTTCATCTGGTCCT IL1 TGGACCTTCCAGGATGAGGACA GTTCATCTCGGAGCCTGTAGTG Ym1 TACTCACTTCCACAGGAGCAGG CTCCAGTGTAGCCATCCTTAGG GTGTAGAAGGTGGAGTTTTGCT C IL13ra1 TGTTCTTGCCGACGCTGTCTAC IL13ra2 TGGTCTGCTCTTGGAAACCTGG GCTGGAGGTAATCAGCACACTG Arg1 TCATCTGGGTGGATGCTCACAC GAGAATCCTGGCACATCGGGAA GCCATAGAACTGATGAGAGGGA G Tnf- GGTGCCTATGTCTCAGCCTCTT Hmox1 CACTCTGGAGATGACACCTGAG GTGTTCCTCTGTCAGCATCACC Tgf- TGATACGCCTGAGTGGCTGTCT CACAAGAGCAGTGAGCGCTGAA Nrf2 CAGCATAGAGCAGGACATGGAG GAACAGCGGTAGTATCAGCCAG Supplemental Table 1: Primer sequence

2. Supplemental Figure 1. PCA analyses of all the study groups.

3. Supplemental Figure 2. Scatter plot showing correlation of various variables and Pearson correlation coefficient (r) of these variables on the top right of the matrix. Blue indicates a negative correlation between the variables while red indicates a positive correlation between the variables.

4. Supplemental Table 2. Spearman Correlation vs Pearson Correlation